1887

Abstract

The hepatitis B virus (HBV) enhancer II (E) is highly liver-specific and plays an important role in regulating the transcription of all HBV genes. In this report, mutational analysis on the B1F-binding site in the major functional unit of HBV E is described. The activity of HBV E in E–CAT reporter plasmids was significantly decreased when the sequence of the B1F-binding site in E was mutated. Furthermore, a single point mutation in the B1 element that aborted the binding of B1F caused a dramatic decrease in viral gene transcription initiated from the HBV core promoter, which resulted in a reduction of the production of the HBV e antigen and pregenomic RNA, the template for viral DNA replication. In conclusion, the interaction of B1F with its target binding sequence in the E region is crucial for liver-specific transcription and DNA replication of the virus.

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2001-03-01
2020-09-29
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References

  1. Ganem D., Varmus H. E.. 1987; The molecular biology of the hepatitis B virus. Annual Review of Biochemistry56:651–693
    [Google Scholar]
  2. Guo W., Chen M., Yen T. S., Ou J. H.. 1993; Hepatocyte-specific expression of the hepatitis B virus core promoter depends on both positive and negative regulation. Molecular and Cellular Biology13:443–448
    [Google Scholar]
  3. Hollinger F. B.. 1996; Hepatitis B virus. In Fields Virology pp2739–2807 Edited by Fields B. N., Knipe D. M., Howley P. M. Philadelphia: Lippincott–Raven;
    [Google Scholar]
  4. Kew M. C.. 1981; The hepatitis B virus and hepatocellular carcinoma. Seminars in Liver Disease1:59–67
    [Google Scholar]
  5. Li M., Xie Y., Wu X., Kong Y., Wang Y.. 1995; HNF3 binds and activates the second enhancer, ENII, of hepatitis B virus. Virology214:371–378
    [Google Scholar]
  6. Li M., Xie Y., Kong Y., Wu X., Zhu L., Wang Y.. 1998; Cloning and characterization of a novel human hepatocyte transcription factor, hB1F, which binds and activates enhancer II of hepatitis B virus. Journal of Biological Chemistry273:29022–29031
    [Google Scholar]
  7. Lopez-Cabrera M., Letovsky J., Hu K. Q., Siddiqui A.. 1990; Multiple liver-specific factors bind to the hepatitis B virus core/pregenomic promoter: trans -activation and repression by CCAAT/enhancer-binding protein. Proceedings of the National Academy of Sciences, USA87:5069–5073
    [Google Scholar]
  8. Lopez-Cabrera M., Letovsky J., Hu K. Q., Siddiqui A.. 1991; Transcriptional factor C/EBP binds to and transactivates the enhancer element II of the hepatitis B virus. Virology183:825–829
    [Google Scholar]
  9. Raney A. K., Zhang P., McLachlan A.. 1995; Regulation of transcription from the hepatitis B virus large surface antigen promoter by hepatocyte nuclear factor 3. Journal of Virology69:3265–3272
    [Google Scholar]
  10. Raney A. K., Johnson J. L., Palmer C. N., McLachlan A.. 1997; Members of the nuclear receptor superfamily regulate transcription from the hepatitis B virus nucleocapsid promoter. Journal of Virology71:1058–1071
    [Google Scholar]
  11. Schaller H., Fischer M.. 1991; Transcriptional control of hepadnavirus gene expression. Current Topics in Microbiology and Immunology168:21–39
    [Google Scholar]
  12. Shaul Y., Rutter W. J., Laub O.. 1985; A human hepatitis B viral enhancer element. EMBO Journal4:427–430
    [Google Scholar]
  13. Summers J., Mason W. S.. 1982; Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate. Cell29:403–415
    [Google Scholar]
  14. Tiollais P., Pourcel C., Dejean A.. 1985; The hepatitis B virus. Nature317:489–495
    [Google Scholar]
  15. Tognoni A., Cattaneo R., Serfling E., Schaffner W.. 1985; A novel expression selection approach allows precise mapping of the hepatitis B virus enhancer. Nucleic Acids Research13:7457–7472
    [Google Scholar]
  16. Wang Y., Chen P., Wu X., Sun A. L., Wang H., Zhu Y. A., Li Z. P.. 1990; A new enhancer element, ENII, identified in the X gene of hepatitis B virus. Journal of Virology64:3977–3981
    [Google Scholar]
  17. Weiss L., Kekule A. S., Jakubowski U., Bürgelt E., Hofschneider P. H.. 1996; The HBV-producing cell line HepG2–4A5: a new in vitro system for studying the regulation of HBV replication and for screening anti-hepatitis B virus drugs. Virology216:214–218
    [Google Scholar]
  18. Will H., Reiser W., Weimer T., Pfaff E., Buscher M., Sprengle R., Cattaneo R., Schaller H.. 1987; Replication strategy of human hepatitis B virus. Journal of Virology61:904–911
    [Google Scholar]
  19. Wu X., Zhu L., Li Z. P., Koshy R., Wang Y.. 1992; Functional organization of enhancer (ENII) of hepatitis B virus. Virology191:490–494
    [Google Scholar]
  20. Yee J. K.. 1989; A liver-specific enhancer in the core promoter region of human hepatitis B virus. Science246:658–661
    [Google Scholar]
  21. Yuh C. H., Ting L. P.. 1991; C/EBP-like proteins binding to the functional box-alpha and box-beta of the second enhancer of hepatitis B virus. Molecular and Cellular Biology11:5044–5052
    [Google Scholar]
  22. Zhang P., Raney A. K., McLachlan A.. 1993; Characterization of functional SP1 transcription factor binding sites in the hepatitis B virus nucleocapsid promoter. Journal of Virology67:1472–1481
    [Google Scholar]
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