@article{mbs:/content/journal/jgv/10.1099/0022-1317-82-3-531, author = "Xie, Youhua and Li, Mei and Wang, Yuan and Hofschneider, Peter Hans and Weiss, Ludwig", title = "Site-specific mutation of the hepatitis B virus enhancer II B1 element: effect on virus transcription and replication", journal= "Journal of General Virology", year = "2001", volume = "82", number = "3", pages = "531-535", doi = "https://doi.org/10.1099/0022-1317-82-3-531", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-82-3-531", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The hepatitis B virus (HBV) enhancer II (EII) is highly liver-specific and plays an important role in regulating the transcription of all HBV genes. In this report, mutational analysis on the B1F-binding site in the major functional unit of HBV EII is described. The activity of HBV EII in EIIā€“CAT reporter plasmids was significantly decreased when the sequence of the B1F-binding site in EII was mutated. Furthermore, a single point mutation in the B1 element that aborted the binding of B1F caused a dramatic decrease in viral gene transcription initiated from the HBV core promoter, which resulted in a reduction of the production of the HBV e antigen and pregenomic RNA, the template for viral DNA replication. In conclusion, the interaction of B1F with its target binding sequence in the EII region is crucial for liver-specific transcription and DNA replication of the virus.", }