1887

Abstract

(PLRV) is transmitted by aphids in a persistent manner. Although virus circulation within the aphid leading to transmission has been well characterized, the mechanisms involved in virus recognition at aphid membranes are still poorly understood. One isolate in our collection (PLRV-14.2) has been shown to be non- or only poorly transmitted by some clones of aphids belonging to the complex. To determine where the transmission process was blocked within the aphid, three virus transmission procedures were used. PLRV-14.2 could not be transmitted, or was only very poorly transmitted, after acquisition from infected plants or from purified preparations. In contrast, it could be transmitted with more than 70% efficiency when microinjected. Therefore, it is concluded that the gut membrane was a barrier regulating passage of PLRV particles from the gut lumen into the haemocoel of . Comparison of coat protein (CP) and readthrough protein (RTP) sequences between poorly and readily transmissible isolates showed that PLRV-14.2 differed from other PLRV isolates by amino acid changes in both of these proteins. It is hypothesized that at least some of the changes found in CP and/or RTP reduced virus recognition by aphid gut receptors, resulting in reduced acquisition and subsequent transmission of PLRV-14.2.

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2001-01-01
2020-01-28
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References

  1. Bahner, I., Lamb, J., Mayo, M. A. & Hay, R. T. ( 1990; ). Expression of the genome of potato leafroll virus: readthrough of the coat protein termination codon in vivo. Journal of General Virology 71, 2251-2256.[CrossRef]
    [Google Scholar]
  2. Björling, K. & Ossiannilsson, F. ( 1958; ). Investigations on individual variations in the virus-transmitting ability of different aphid species. Socker 14, 1-13.
    [Google Scholar]
  3. Bourdin, D., Rouzé, J., Tanguy, S. & Robert, Y. ( 1998; ). Variation among clones of Myzus persicae (Sulzer) and Myzus nicotianae Blackman in the transmission of a poorly- and a highly aphid-transmissible isolate of potato leafroll luteovirus (PLRV). Plant Pathology 47, 794-800.
    [Google Scholar]
  4. Brault, V., van den Heuvel, J. F. J. M., Verbeek, M., Ziegler-Graff, V., Reutenauer, A., Herrbach, E., Garaud, J.-C., Guilley, H., Richards, K. & Jonard, G. ( 1995; ). Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74. EMBO Journal 14, 650-659.
    [Google Scholar]
  5. Brault, V., Mutterer, J. D., Scheidecker, D., Simonis, M. T., Herrbach, E., Richards, K. & Ziegler-Graff, V. ( 2000; ). Effects of point mutations in the readthrough domain of the beet western yellows virus minor capsid protein on virus accumulation in planta and aphid transmission. Journal of Virology 74, 1140-1148.[CrossRef]
    [Google Scholar]
  6. Bruyère, A., Brault, V., Ziegler-Graff, V., Simonis, M. T., van den Heuvel, J. F. J. M., Richards, K., Guilley, H., Jonard, G. & Herrbach, E. ( 1997; ). Effects of mutations in the beet western yellows virus readthrough protein on its expression and packaging and on virus accumulation, symptoms and aphid transmission. Virology 230, 323-334.[CrossRef]
    [Google Scholar]
  7. Chay, C. A., Gunasinge, U. B., Dinesh-Kumar, S. P., Miller, W. A. & Gray, S. M. ( 1996; ). Aphid transmission and systemic plant infection determinants of barley yellow dwarf luteovirus-PAV are contained in the coat protein readthrough domain and 17-kDa protein respectively. Virology 219, 57-65.[CrossRef]
    [Google Scholar]
  8. Chuquillanqui, C. & Jones, R. A. C. ( 1980; ). A rapid technique for assessing the resistance of families of potato seedlings to potato leaf roll virus. Potato Research 23, 121-128.[CrossRef]
    [Google Scholar]
  9. Clark, M. F. & Adams, A. N. ( 1977; ). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34, 475-483.[CrossRef]
    [Google Scholar]
  10. Garret, A., Kerlan, C. & Thomas, D. ( 1993; ). The intestine is a site of passage for potato leafroll virus from the gut lumen into the haemocoel in the aphid vector, Myzus persicae Sulz. Archives of Virology 131, 377-392.[CrossRef]
    [Google Scholar]
  11. Gildow, F. E. ( 1982; ). Coated vesicle transport of luteovirus through the salivary gland of Myzus persicae. Phytopathology 72, 1289-1296.[CrossRef]
    [Google Scholar]
  12. Gildow, F. E. ( 1993; ). Evidence for receptor-mediated endocytosis regulating luteovirus acquisition by aphids. Phytopathology 83, 270-277.[CrossRef]
    [Google Scholar]
  13. Gildow, F. E. ( 1999; ). Luteovirus transmission mechanisms regulating vector specificity. In The Luteoviridae , pp. 88-111. Edited by H. G. Smith& H. Barker. Wallingford:CABI.
  14. Gildow, F. E. & Gray, S. M. ( 1993; ). The aphid salivary gland basal lamina as a selective barrier associated with vector-specific transmission of barley yellow dwarf luteoviruses. Phytopathology 83, 1293-1302.[CrossRef]
    [Google Scholar]
  15. Gildow, F. E. & Rochow, W. F. ( 1980; ). Role of accessory salivary glands in aphid transmission of barley yellow dwarf virus. Virology 104, 97-108.[CrossRef]
    [Google Scholar]
  16. Gildow, F. E., Damsteegt, V. D., Bingaman, D. & Powell, C. ( 1994; ). Cellular mechanisms regulating circulative transmission and aphid vector specificity of soybean dwarf luteoviruses. Phytopathology 84, 1155-1156.
    [Google Scholar]
  17. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95-98.
    [Google Scholar]
  18. Hinz, B. ( 1966; ). Versuche zur Übertragung des Blattrollvirus der Kartoffel durch die grünstreifige Kartoffelblattlaus Macrosiphum euphorbiae (Thomas). Wissenschaftliche Zeitschrift der Universität Rostock. Mathematische-naturwissenschaftliche Reihe 15, 289-293.
    [Google Scholar]
  19. Jolly, C. A. & Mayo, M. A. ( 1994; ). Changes in the amino acid sequence of the coat protein readthrough domain of potato leafroll luteovirus affect the formation of an epitope and aphid transmission. Virology 201, 182-185.[CrossRef]
    [Google Scholar]
  20. Keese, P., Martin, R. R., Kawchuk, L. M., Waterhouse, P. M. & Gerlach, W. L. ( 1990; ). Nucleotide sequences of an Australian and a Canadian isolate of potato leafroll luteovirus and their relationships with two European isolates. Journal of General Virology 71, 719-724.[CrossRef]
    [Google Scholar]
  21. Kennedy, J. S., Day, M. F. & Eastop, V. F. (1962). A conspectus of aphids as vectors of plant viruses. London: Commonwealth Institute of Entomology.
  22. Mayo, M. A. & D’Arcy, C. J. ( 1999; ). Family Luteoviridae: A reclassification of Luteoviruses. In The Luteoviridae , pp. 15-22. Edited by H. G. Smith& H. Barker. Wallingford:CABI.
  23. Mayo, M. A. & Ziegler-Graff, V. ( 1996; ). Molecular biology of luteoviruses. Advances in Virus Research 46, 413-460.
    [Google Scholar]
  24. Palucha, A., Sadowy, E., Kujawa, A., Juszczuk, M., Zagórski, W. & Hulanicka, D. ( 1994; ). Nucleotide sequence of RNA of a Polish isolate of potato leafroll luteovirus. Acta Biochemica Polonica 41, 405-414.
    [Google Scholar]
  25. Peiffer, M. L., Gildow, F. E. & Gray, S. M. ( 1997; ). Two distinct mechanisms regulate luteovirus transmission efficiency and specificity at the aphid salivary gland. Journal of General Virology 78, 495-503.
    [Google Scholar]
  26. Robert, Y. ( 1971; ). Epidémiologie de l’Enroulement de la pomme de terre: capacité vectrice de stades et de formes des pucerons Aulacorthum solani Kltb, Macrosiphum euphorbiae Thomas et Myzus persicae Sulz. Potato Research 14, 130-139.[CrossRef]
    [Google Scholar]
  27. Robert, Y. & Maury, Y. ( 1970; ). Capacités vectrices comparées de plusieurs souches de Myzus persicae Sulz., Aulacorthum solani Kltb et Macrosiphum euphorbiae Thomas dans l’étude de la transmission de l’Enroulement de la pomme de terre. Potato Research 13, 199-209.[CrossRef]
    [Google Scholar]
  28. Rochow, W. F. ( 1969; ). Biological properties of four isolates of barley yellow dwarf virus. Phytopathology 59, 1580-1589.
    [Google Scholar]
  29. Rochow, W. F., Foxe, M. J. & Muller, I. ( 1975; ). A mechanism of vector specificity for circulative aphid-transmitted plant viruses. Annals of the New York Academy of Sciences 266, 293-301.[CrossRef]
    [Google Scholar]
  30. SAS Institute Inc. (1995). SAS/STAT User’s Guide, release 6.03. Cary, NC: SAS Institute Inc.
  31. Takanami, Y. & Kubo, S. ( 1979; ). Enzyme-assisted purification of two phloem-limited plant viruses: tobacco necrotic dwarf and potato leafroll. Journal of General Virology 44, 153-159.[CrossRef]
    [Google Scholar]
  32. Tamada, T. & Harrison, B. D. ( 1980; ). Application of enzyme-linked immunosorbent assay to the detection of potato leafroll virus in potato tubers. Annals of Applied Biology 96, 67-78.[CrossRef]
    [Google Scholar]
  33. Tamada, T., Harrison, B. D. & Roberts, I. M. ( 1984; ). Variation among British isolates of potato leafroll virus. Annals of Applied Biology 104, 107-116.[CrossRef]
    [Google Scholar]
  34. Terradot, L., Simon, J. C., Leterme, N., Bourdin, D., Wilson, A. C. C., Gauthier, J. P. & Robert, Y. ( 1999; ). Molecular characterization of clones of the Myzus persicae complex (Hemiptera: Aphididae) differing in their ability to transmit the potato leafroll luteovirus (PLRV). Bulletin of Entomological Research 89, 355-363.
    [Google Scholar]
  35. Upreti, G. C. & Nagaich, B. B. ( 1971; ). Variations in the ability of Myzus persicae Sulz. to transmit potato viruses. I. Leaf roll. Phytopathologische Zeitschrift 71, 163-168.[CrossRef]
    [Google Scholar]
  36. van den Heuvel, J. F. J. M., Verbeek, M. & Peters, D. ( 1993; ). The relationship between aphid-transmissibility of potato leafroll virus and surface epitopes of the viral capsid. Phytopathology 83, 1125-1129.[CrossRef]
    [Google Scholar]
  37. van den Heuvel, J. F. J. M., Verbeek, M. & van der Wilk, F. ( 1994; ). Endosymbiotic bacteria associated with circulative transmission of potato leafroll virus by Myzus persicae. Journal of General Virology 75, 2559-2565.[CrossRef]
    [Google Scholar]
  38. van den Heuvel, J. F. J. M., de Blank, C. M., Peters, D. & van Lent, J. W. M. ( 1995; ). Localization of potato leafroll virus in leaves of secondarily-infected potato plants. European Journal of Plant Pathology 101, 567-571.[CrossRef]
    [Google Scholar]
  39. van der Wilk, F., Huisman, M. J., Cornelissen, B. J. C., Huttinga, H. & Goldbach, R. ( 1989; ). Nucleotide sequence and organization of potato leafroll virus genomic RNA. FEBS Letters 245, 51-56.[CrossRef]
    [Google Scholar]
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