1887

Abstract

The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)H:gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gD were determined. As antigens, purified soluble recombinant forms of the gH:gL complex produced by insect cells and of gD produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gH: gL-specific IgG. Using peripheral blood (PB) T cells, gH:gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gH:gL contained high levels of interferon-γ and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.

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2000-08-01
2020-01-17
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