1887

Journal of General Virology header logo

Volume 81, Issue 8

Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex Free

Abstract

The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)H:gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gD were determined. As antigens, purified soluble recombinant forms of the gH:gL complex produced by insect cells and of gD produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gH: gL-specific IgG. Using peripheral blood (PB) T cells, gH:gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gH:gL contained high levels of interferon-γ and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.

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© Microbiology Society
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/content/journal/jgv/10.1099/0022-1317-81-8-2011
2000-08-01
2023-01-29
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References

  1. Beninga J., Kropff B., Mach M. 1995; Comparative analysis of fourteen individual human cytomegalovirus proteins for helper T cell response. Journal of General Virology 76:153–160
     [Google Scholar]
  2. Beninga J., Kalbacher H., Mach M. 1996; Analysis of T helper cell response to glycoprotein H (gpUL75) of human cytomegalovirus: evidence for strain-specific T cell determinants. Journal of Infectious Diseases 173:1051–1061
     [Google Scholar]
  3. Biron C. A. 1994; Cytokines in the generation of immune responses to, and resolution of, virus infection. Current Opinion in Immunology 6:530–538
     [Google Scholar]
  4. Browne H., Baxter V., Minson T. 1993; Analysis of protective immune responses to the glycoprotein H–glycoprotein L complex of herpes simplex virus type 1. Journal of General Virology 74:2813–2817
     [Google Scholar]
  5. Buckmaster E. A., Gompels U., Minson A. 1984; Characterization and physical mapping of an HSV-1 glycoprotein of approximately 115×103 molecular weight. Virology 139:408–413
     [Google Scholar]
  6. Carmack M. A., Yasukawa L. L., Chang S. Y., Tran C., Saldana F., Arvin A. M., Prober C. G. 1996; T cell recognition and cytokine production elicited by common and type-specific glycoproteins of herpes simplex virus type 1 and type 2. Journal of Infectious Diseases 174:899–906
     [Google Scholar]
  7. Cher D. J., Mosmann T. R. 1987; Two types of murine helper T cell clone. II. Delayed-type hypersensitivity is mediated by TH1 clones. Journal of Immunology 138:3688–3694
     [Google Scholar]
  8. Forrester A. J., Sullivan V., Simmons A., Blacklaws B. A., Smith G. L., Nash A. A., Minson A. C. 1991; Induction of protective immunity with antibody to herpes simplex virus type 1 glycoprotein H (gH) and analysis of the immune response to gH expressed in recombinant vaccinia virus. Journal of General Virology 72:369–375
     [Google Scholar]
  9. Forrester A., Farrell H., Wilkinson G., Kaye J., Davis-Poynter N., Minson T. 1992; Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted. Journal of Virology 66:341–348
     [Google Scholar]
  10. Fuller A. O., Lee W. C. 1992; Herpes simplex virus type 1 entry through a cascade of virus–cell interactions requires different roles of gD and gH in penetration. Journal of Virology 66:5002–5012
     [Google Scholar]
  11. Ghiasi H., Kaiwar R., Nesburn A. B., Wechsler S. L. 1992; Baculovirus-expressed glycoprotein H of herpes simplex virus type 1 (HSV-1) induces neutralizing antibody and delayed type hypersensitivity responses, but does not protect immunized mice against lethal HSV-1 challenge. Journal of General Virology 73:719–722
     [Google Scholar]
  12. Ghiasi H., Kaiwar R., Nesburn A. B., Slanina S., Wechsler S. L. 1994a; Expression of seven herpes simplex virus type 1 glycoproteins (gB, gC, gD, gE, gG, gH, and gI): comparative protection against lethal challenge in mice. Journal of Virology 68:2118–2126
     [Google Scholar]
  13. Ghiasi H., Kaiwar R., Slanina S., Nesburn A. B., Wechsler S. L. 1994b; Expression and characterization of baculovirus expressed herpes simplex virus type 1 glycoprotein L. Archives of Virology 138:199–212
     [Google Scholar]
  14. Hendricks R. L., Tumpey T. M., Finnegan A. 1992; IFN-gamma and IL-2 are protective in the skin but pathologic in the corneas of HSV-1-infected mice. Journal of Immunology 149:3023–3028
     [Google Scholar]
  15. Hill A., Jugovic P., York I., Russ G., Bennink J., Yewdell J., Ploegh H., Johnson D. 1995; Herpes simplex virus turns off the TAP to evade host immunity. Nature 375:411–415
     [Google Scholar]
  16. Hutchinson L., Browne H., Wargent V., Davis-Poynter N., Primorac S., Goldsmith K., Minson A. C., Johnson D. C. 1992; A novel herpes simplex virus glycoprotein, gL, forms a complex with glycoprotein H (gH) and affects normal folding and surface expression of gH. Journal of Virology 66:2240–2250
     [Google Scholar]
  17. Koelle D. M., Corey L., Burke R. L., Eisenberg R. J., Cohen G. H., Pichyangkura R., Triezenberg S. J. 1994; Antigenic specificities of human CD4+ T-cell clones recovered from recurrent genital herpes simplex virus type 2 lesions. Journal of Virology 68:2803–2810
     [Google Scholar]
  18. Mikloska Z., Cunningham A. L. 1998; Herpes simplex virus type 1 glycoproteins gB, gC and gD are major targets for CD4 T-lymphocyte cytotoxicity in HLA-DR expressing human epidermal keratinocytes. Journal of General Virology 79:353–361
     [Google Scholar]
  19. Oda-Ikoma M., Glazenburg K. L., The T. H., Welling-Wester S. 1998; A fragment of glycoprotein G of herpes simplex virus type 2 (gG2) expressed in the baculovirus expression system for detection of HSV-2 antibodies. Abstract 189: 23rd International Herpesvirus Workshop 1–7 August, 1998 York, UK:
     [Google Scholar]
  20. Peng T., Ponce de Leon M., Jiang H., Dubin G., Lubinski J. M., Eisenberg R. J., Cohen G. H. 1998; The gH–gL complex of herpes simplex virus (HSV) stimulates neutralizing antibody and protects mice against HSV type 1 challenge. Journal of Virology 72:65–72
     [Google Scholar]
  21. Posavad C. M., Koelle D. M., Corey L. 1996; High frequency of CD8+ cytotoxic T-lymphocyte precursors specific for herpes simplex viruses in persons with genital herpes. Journal of Virology 70:8165–8168
     [Google Scholar]
  22. Posavad C. M., Koelle D. M., Corey L. 1998; Tipping the scales of herpes simplex virus reactivation: the important responses are local. Nature Medicine 4:381–382
     [Google Scholar]
  23. Roop C., Hutchinson L., Johnson D. C. 1993; A mutant herpes simplex virus type 1 unable to express glycoprotein L cannot enter cells, and its particles lack glycoprotein H. Journal of Virology 67:2285–2297
     [Google Scholar]
  24. Schmid D. S. 1988; The human MHC-restricted cellular response to herpes simplex virus type 1 is mediated by CD4+, CD8− T cells and is restricted to the DR region of the MHC complex. Journal of Immunology 140:3610–3616
     [Google Scholar]
  25. Showalter S. D., Zweig M., Hampar B. 1981; Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate-early protein ICP4. Infection and Immunity 34:684–692
     [Google Scholar]
  26. Simmons A., Nash A. A. 1985; Role of antibody in primary and recurrent herpes simplex virus infection. Journal of Virology 53:944–948
     [Google Scholar]
  27. Urban M., Klein M., Britt W. J., Haßfurther E., Mach M. 1996; Glycoprotein H of human cytomegalovirus is a major antigen for the neutralizing humoral immune response. Journal of General Virology 77:1537–1547
     [Google Scholar]
  28. Verjans G. M., Feron E. J., Dings M. E., Cornelissen J. G., van der Lelij A., Baarsma G. S., Osterhaus A. D. 1998a; T cells specific for the triggering virus infiltrate the eye in patients with herpes simplex virus-mediated acute retinal necrosis. Journal of Infectious Diseases 178:27–34
     [Google Scholar]
  29. Verjans G. M., Remeijer L., van Binnendijk R. S., Cornelissen J. G., Volker-Dieben H. J., Baarsma S. G., Osterhaus A. D. 1998b; Identification and characterization of herpes simplex virus-specific CD4+ T cells in corneas of herpetic stromal keratitis patients. Journal of Infectious Diseases 177:484–488
     [Google Scholar]
  30. York I. A., Roop C., Andrews D. W., Riddell S. R., Graham F. L., Johnson D. C. 1994; A cytosolic herpes simplex virus protein inhibits antigen presentation to CD8+ T lymphocytes. Cell 77:525–535
     [Google Scholar]
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Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex
J. Gen. Virol. 81, 2011 (2000); https://doi.org/10.1099/0022-1317-81-8-2011
/content/journal/jgv/10.1099/0022-1317-81-8-2011
/content/journal/jgv/10.1099/0022-1317-81-8-2011
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