1887

Journal of General Virology

Volume 81, Issue 8

Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex Free

Abstract

The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)H:gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gD were determined. As antigens, purified soluble recombinant forms of the gH:gL complex produced by insect cells and of gD produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gH: gL-specific IgG. Using peripheral blood (PB) T cells, gH:gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gH:gL contained high levels of interferon-γ and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.

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© Microbiology Society
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2000-08-01
2020-01-17
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Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex
J. Gen. Virol. 81, 2011 (2000); https://doi.org/10.1099/0022-1317-81-8-2011
/content/journal/jgv/10.1099/0022-1317-81-8-2011
/content/journal/jgv/10.1099/0022-1317-81-8-2011
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