@article{mbs:/content/journal/jgv/10.1099/0022-1317-81-8-2011, author = "Westra, Douwe F. and Verjans, Georges M. G. M. and Osterhaus, Albert D. M. E. and van Kooij, Adriaan and Welling, Gjalt W. and Scheffer, Albert Jan and The, T. Hauw and Welling-Wester, Sytske", title = "Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex", journal= "Journal of General Virology", year = "2000", volume = "81", number = "8", pages = "2011-2015", doi = "https://doi.org/10.1099/0022-1317-81-8-2011", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-81-8-2011", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)Ht(His):gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gDt were determined. As antigens, purified soluble recombinant forms of the gHt(His):gL complex produced by insect cells and of gDt produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gHt(His): gL-specific IgG. Using peripheral blood (PB) T cells, gHt(His):gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gHt(His):gL contained high levels of interferon-γ and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.", }