1887

Abstract

Since it was first described in Australia in 1994, Hendra virus (HeV) has caused two outbreaks of fatal disease in horses and humans, and an isolated fatal horse case. Our preliminary studies revealed a high prevalence of neutralizing antibodies to HeV in bats of the genus , but it was unclear whether this was due to infection with HeV or a related virus. We developed the hypothesis that HeV excretion from bats might be related to the birthing process and we targeted the reproductive tract for virus isolation. Three virus isolates were obtained from the uterine fluid and a pool of foetal lung and liver from one grey-headed flying-fox (), and from the foetal lung of one black flying-fox (). Antigenically, these isolates appeared to be closely related to HeV, returning positive results on immunofluorescent antibody staining and constant-serum varying-virus neutralization tests. Using an HeV-specific oligonucleotide primer pair, genomic sequences of the isolates were amplified. Sequencing of 200 nucleotides in the matrix gene identified that these three isolates were identical to HeV. Isolations were confirmed after RNA extracted from original material was positive for HeV RNA when screened on an HeV Taqman assay. The isolation of HeV from pteropid bats corroborates our earlier serological and epidemiological evidence that they are a natural reservoir host of the virus.

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2000-08-01
2024-11-05
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References

  1. Aziz J., Olson J., Lee O. B., Daniels P., Adzhar A. B., Bunning M., Field H., Johara M. Y., Hooper P. 1999; Nipah virus infection of animals in Malaysia. In Abstracts of the XIth International Congress of Virology, Sydney, Australia, (9th–13th August, 1999} Abstract VW31B.02.p 38
    [Google Scholar]
  2. Corbet G. B., Hill J. E. 1992 The Mammals of the Indomalay Region: a Systematic Review Oxford: Oxford University Press;
  3. Field H., Mohd Yob J., Morrissy C., Selleck P. 1999; Nipah virus in Malaysia – preliminary wildlife surveillance. In Abstracts of the XIth International Congress of Virology, Sydney, Australia (9th–13th August, 1999) Abstract VP31 21 p 187
    [Google Scholar]
  4. Field H., Barrat P. C., Hughes R. J., Shield J., Sullivan N. D. 2000; A fatal case of Hendra virus infection in a horse in north Queensland – clinical and epidemiological features. Australian Veterinary Journal 78:279–280
    [Google Scholar]
  5. Flannery T. F. 1995 Mammals of the South-West Pacific and Moluccan Islands Sydney: Reed Books;
  6. Fraser G. C., Hooper P. T., Lunt R. A., Gould A. R., Gleeson L. J., Hyatt A. D., Russell G. M., Kattenbelt J. A. 1996; Encephalitis caused by a Lyssavirus in fruit bats in Australia. Emerging Infectious Diseases 2:327–331
    [Google Scholar]
  7. Hall L. S. 1986; Identification, distribution and taxonomy of Australian flying foxes. Australian Mammalogy 10:75–79
    [Google Scholar]
  8. Henderson G. W., Laird C., Dermott E., Rima B. K. 1995; Characterization of Mapuera virus: structure, proteins and nucleotide sequence of the gene encoding the nucleocapsid protein. Journal of General Virology 76:2509–2518
    [Google Scholar]
  9. Lye M. S., Parashar U. D., Mounts A. W., Kitsutani P., Taha M., Tee A. S., Suleiman A. B., Ksiazek T. G. 1999; Outbreak of Nipah (Hendra-like) virus encephalitis among humans, Malaysia, 1998–1999. In Abstracts of the XIth International Congress of Virology Sydney Australia (9th–13th August, 1999) Abstract VW31B.04 p 38
    [Google Scholar]
  10. Mickleburgh S. P., Hutson A. M., Racey P. A. 1992 Old World Fruit Bats: an Action Plan for their Conservation Gland: IUCN;
  11. Murray K., Selleck P., Hooper P., Hyatt A., Gould A., Gleeson L., Westbury H., Hiley L., Selvey L., Rodwell B. 1995; A morbillivirus that caused fatal disease in horses and humans. Science 268:94–97
    [Google Scholar]
  12. O’Sullivan J. D., Allworth A. M., Paterson D. L., Snow T. M., Boots R., Gleeson L. J., Gould A. R., Hyatt A. D., Bradfield J. 1997; Fatal encephalitis due to novel paramyxovirus transmitted from horses. Lancet 349:93–95
    [Google Scholar]
  13. Pavri K. M., Singh K. R., Hollinger F. B. 1971; Isolation of a new parainfluenza virus from a frugivorous bat, Rousettus leschenaulti , collected at Poona, India. American Journal of Tropical Medicine and Hygiene 20:125–130
    [Google Scholar]
  14. Philbey A. W., Kirkland P. D., Ross A. D., Davis R. J., Gleeson A. B., Love R. J., Daniels P. W., Gould A. R., Hyatt A. D. 1998; An apparently new virus (family Paramyxoviridae) infectious for pigs, humans, and fruit bats. Emerging Infectious Diseases 4:269–271
    [Google Scholar]
  15. Rogers R. J., Douglas I. C., Baldock F. C., Glanville R. J., Seppanen K. T., Gleeson L. J., Selleck P. N., Dunn K. J. 1996; Investigation of a second focus of equine morbillivirus infection in coastal Queensland. Australian Veterinary Journal 74:243–244
    [Google Scholar]
  16. Rota P., Harcourt B., Rollin P., Widjojoatmodjo M., Bellini W., Chua K. B., Goldsmith C. E., Olson J. J., Bunning M., Tsiazek T., Tamin A. 1999; Molecular characterisation of a Hendra-like virus (Nipah virus) isolated from fatal encephalitis cases in Malaysia and Singapore. In Abstracts of the XIth International Congress of Virology Sydney Australia (9th–13th August, 1999 Abstract VW31B.03 p 38
    [Google Scholar]
  17. Schorr E., Wentworth D., Hinshaw V. S. 1994; Use of polymerase chain reaction to detect swine influenza virus in nasal swab specimens. American Journal of Veterinary Research 55:952–956
    [Google Scholar]
  18. Selvey L. A., Wells R. M., McCormack J. G., Ansford A. J., Murray K., Rogers R. J., Lavercombe P. S., Selleck P., Sheridan J. W. 1995; Infection of humans and horses by a newly described morbillivirus. Medical Journal of Australia 162:642–645
    [Google Scholar]
  19. Selvey L. A., Taylor R., Arklay A., Gerrard J. 1996; Screening of bat carers for antibodies to equine morbillivirus. Communicable Diseases Intelligence 20:477–478
    [Google Scholar]
  20. Sulkin S. E., Allen R. 1974; Virus infections in bats. In Monographs in Virology pp 170–175 Edited by Melnick J. L. New York: S. Karger;
    [Google Scholar]
  21. Wang L. F., Michalski W. P., Yu M., Pritchard L. I., Crameri G., Shiell B., Eaton B. 1998; A novel P/V/C gene in a new member of the Paramyxoviridae family which causes lethal infection in humans, horses and other animals. Journal of Virology 72:1482–1490
    [Google Scholar]
  22. Williamson M. M., Hooper P. T., Selleck P. W., Gleeson L. J., Daniels P. W., Westbury H. A., Murray P. K. 1998; Transmission studies of Hendra virus (equine morbillivirus) in fruit bats, horses and cats. Australian Veterinary Journal 76:813–818
    [Google Scholar]
  23. Young P. L., Halpin K., Selleck P. W., Field H., Gravel J. L., Kelly M. A., Mackenzie J. S. 1996; Serologic evidence for the presence in Pteropus bats of a paramyxovirus related to equine morbillivirus. Emerging Infectious Diseases 2:239–240
    [Google Scholar]
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