1887

Abstract

The brown alga Ectocarpus siliculosus frequently carries an endogenous virus, E. siliculosus virus (EsV-1), the genome of which is a circular, double-stranded DNA molecule of about 320 kbp. After infection, which occurs in the unicellular spores or gametes, the virus is present latently in all somatic cells of the host. Virus multiplication is restricted to cells of the reproductive organs. It has been an open question whether the latent viral DNA occurs as a free episome or becomes integrated into the host genome. PCR studies showed that viral DNA co-migrates with high molecular mass DNA in pulsed-field gel electrophoresis, which confirms that latent viral DNA is integrated into the host genome.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-6-1367
1999-06-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/6/0801367a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-6-1367&mimeType=html&fmt=ahah

References

  1. Boehmer P. E., Lehman I. R. 1997; Herpes simplex virus DNA replication. Annual Review of Biochemistry 66:347–384
    [Google Scholar]
  2. Bräutigam M., Klein M., Knippers R., Müller D. G. 1995; Inheritance and meiotic elimination of a virus genome in the host Ectocarpus siliculosus (Phaeophyceae). Journal of Phycology 31:823–827
    [Google Scholar]
  3. Del Campo E., Ramazanov Z., Garcia-Reina G., Muller D. G. 1997; Photosynthetic responses and growth performance of virus-infected and non-infected Ectocarpus siliculosus (Phaeophyceae). Phycologia 36:186–189
    [Google Scholar]
  4. Klein M., Lanka S., Müller D., Knippers R. 1994; Single-stranded regions in the genome of the Ectocarpus siliculosus virus. Virology 202:1076–1078
    [Google Scholar]
  5. Lanka S. T. J., Klein M., Ramsperger U., Müller D. G., Knippers R. 1993; Genome structure of a virus infecting the marine brown alga Ectocarpus siliculosus. Virology 193:802–811
    [Google Scholar]
  6. Lee A. M., Ivey R. G., Meints R. H. 1998; Repetitive DNA insertion in a protein kinase ORF of a latent FSV (Feldmannia sp. virus) genome. Virology 248:35–45
    [Google Scholar]
  7. Liu Y. G., Whittier R. F. 1994; Rapid preparation of megabase plant DNA from nuclei in agarose plugs and microbeads. Nucleic Acids Research 22:2168–2169
    [Google Scholar]
  8. Mosig G., Colowick N., Gruidl M. E., Chang A., Harvey A. J. 1995; Multiple initiation mechanisms adapt phage T4 DNA replication to physiological changes during T4′s development. FEMS Microbiology Reviews 17:83–98
    [Google Scholar]
  9. Müller D. G. 1979; Genetic affinity of Ectocarpus siliculosus (Dillw.) Lyngb. from the Mediterranean, North Atlantic and Australia. Phycologia 18:312–318
    [Google Scholar]
  10. Müller D. G. 1991a; Mendelian segregation of a virus genome during host meiosis in the marine brown alga Ectocarpus siliculosus. Journal of Plant Physiology 137:739–743
    [Google Scholar]
  11. Müller D. G. 1991b; Marine virioplankton produced by infected Ectocarpus siliculosus (Phaeophyceae). Marine Ecology Progress Series 76:101–102
    [Google Scholar]
  12. Müller D. G., Kawai H., Stache B., Lanka S. 1990; A virus infection in the marine brown alga Ectocarpus siliculosus (Phaeophyceae). Botanica Acta 103:72–82
    [Google Scholar]
  13. Müller D. G., Sengco M., Wolf S., Bräutigam M., Schmid C. E., Kapp M., Knippers R. 1996; Comparison of two DNA viruses infecting the marine brown algae Ectocarpus siliculosus and E. fasciculatus. Journal of General Virology 77:2329–2333
    [Google Scholar]
  14. Müller D. G., Kapp M., Knippers R. 1998; Viruses in marine brown algae. Advances in Virus Research 50:49–67
    [Google Scholar]
  15. Sengco M. R., Bräutigam M., Kapp M., Müller D. G. 1996; Detection of virus DNA in Ectocarpus siliculosus and E. fasciculatus (Phaeophyceae) from various geographic areas. European Journal of Phycology 31:73–78
    [Google Scholar]
  16. Zimmer R., Verrinder Gibbins A. M. 1997; Construction and characterization of a large-fragment chicken bacterial artificial chromosome library. Genomics 42217–226
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-6-1367
Loading
/content/journal/jgv/10.1099/0022-1317-80-6-1367
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error