1887

Abstract

Porcine adenovirus-3 (PAV-3) was developed as an expression vector using homologous recombination in Escherichia coli BJ 5183. As a prerequisite, the complete genome of PAV-3 was first introduced as a PacI restriction fragment into a bacterial plasmid. The plasmid, when PacI restricted and transfected into swine testicular cells, produces an infectious virus. The potential of this procedure was demonstrated by the construction of several PAV-3 recombinants. Part of the E3 region, which is nonessential for virus replication under cell culture conditions, was identified and deleted from the virus genome. The gene for glycoprotein D (gD) of pseudorabies virus (PRV), which elicits PRV-neutralizing antibodies in pigs, was cloned and expressed from the E3 region of PAV-3. A 50 kDa polypeptide was identified in recombinant PAV-3-infected cell lysates by immunoprecipitation assays using gD-specific monoclonal antibodies. In another experiment, a region between the right inverted terminal repeat and the promoter of the E4 region was used to clone and express the chloramphenicol acetyltransferase (CAT) gene under the control of SV40 immediate early promoter. CAT gene expression was observed irrespective of the orientation of the CAT gene. These results indicate that the helper-independent recombinant PAV-3 could be used as an expression vector and has potential as a recombinant vaccine vector in pigs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-3-563
1999-03-01
2022-08-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/3/0800563a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-3-563&mimeType=html&fmt=ahah

References

  1. Berkner K. L. 1988; Development of adenovirus vectors for expression of heterologous genes. BioTechniques 6:616–629
    [Google Scholar]
  2. Bett A. J., Haddara W., Prevec L., Graham F. L. 1994; An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proceedings of the National Academy of Sciences, USA 91:8802–8806
    [Google Scholar]
  3. Chartier C., Degryse E., Gantzer M., Dieterle A., Pavirani A., Mehtali M. 1996; Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli. Journal of Virology 70:4805–4810
    [Google Scholar]
  4. Clarke M. C., Sharpe H. B. A., Derbyshire J. B. 1967; Some characteristics of three porcine adenoviruses. Archiv fur die Gesamte Virusforschung 21:91–97
    [Google Scholar]
  5. Derbyshire J. B., Clarke M. C., Collins A. P. 1975; Serological and pathogenicity studies with some unclassified porcine adenoviruses. Journal of Comparative Pathology 85:437–443
    [Google Scholar]
  6. Evans P. S., Benko M., Harrach B., Letchworth G. 1998; Sequence, transcriptional analysis, and deletion of the bovine adenovirus type 1 E3 region. Virology 244:173–185
    [Google Scholar]
  7. Ghosh-Choudhury G., Haj-Ahmad Y., Graham F. L. 1987; Protein IX, a minor component of the human adenovirus capsid, is essential for the packaging of full length genomes. EMBO Journal 6:1733–1739
    [Google Scholar]
  8. Graham F. L., Prevec L. 1991; Manipulation of adenovirus vectors. Methods in Molecular Biology 7:109–128
    [Google Scholar]
  9. Hirahara T., Yauhara H., Matsui O., Yamanaka M., Tanaka M., Fukuyama S. 1990; Isolation of porcine adenovirus from the respiratory tract of pigs in Japan. Japanese Journal of Veterinary Sciences 52:407–409
    [Google Scholar]
  10. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  11. Ishibashi M., Yasue H. 1984; Adenoviruses of animals. In The Adenoviruses pp 497–562 Edited by Ginsberg H. S. New York: Plenum Press;
    [Google Scholar]
  12. Ketner G., Spencer F., Tugendriech S., Connelly C., Hieter P. 1994; Efficient manipulation of the human adenovirus genome as an infectious yeast artificial chromosome clone. Proceedings of the National Academy of Sciences, USA 91:6186–6190
    [Google Scholar]
  13. Klonjkowski B., Gillardi-Hebenstreit P., Hadchouel J., Randrianarison V., Boutin S., Yeh P., Perricaudet M., Kremer E. J. 1997; A recombinant E1-deleted canine adenoviral vector capable of transduction and expression of a transgene in human derived cells and in vivo. Human Gene Therapy 8:2103–2115
    [Google Scholar]
  14. McCoy R. J., Johnson M. A., Studdert M. J., Sheppard M. 1996a; Genomic location and nucleotide sequence of a porcine adenovirus penton base gene. Archives of Virology 141:1367–1375
    [Google Scholar]
  15. McCoy R. J., Johnson M. A., Sheppard M. 1996b; Nucleotide and amino acid sequence analysis of the porcine adenovirus 23K protein. DNA Sequence 6:251–254
    [Google Scholar]
  16. McCoy R. J., Johnson M. A., Sheppard M. 1997; Nucleotide and amino acid sequence analysis of the 100K protein of a serotype 3 porcine adenovirus. DNA Sequence 8:59–61
    [Google Scholar]
  17. Makimura M., Miyake S., Akino N., Takamori K., Matsuura Y., Miyamura T., Saito I. 1996; Induction of antibodies against structural proteins of hepatitis C virus in mice using recombinant adenovirus. Vaccine 14:28–34
    [Google Scholar]
  18. Mittal S. K., Prevec L., Graham F. L., Babiuk L. A. 1995; Development of a bovine adenovirus type 3-based expression vector. Journal of General Virology 76:93–102
    [Google Scholar]
  19. Reddy P. S., Nagy E., Derbyshire J. B. 1993; Restriction endonuclease analysis and molecular cloning of porcine adenovirus type 3. Intervirology 36:161–168
    [Google Scholar]
  20. Reddy P. S., Nagy E., Derbyshire J. B. 1995a; Sequence analysis of putative pVIII, E3 and fibre regions of porcine adenovirus type 3. Virus Research 36:97–106
    [Google Scholar]
  21. Reddy P. S., Tuboly T., Dennis J., Derbyshire J. B., Nagy E. 1995b; Comparison of inverted terminal repetition sequences from five porcine adenovirus serotypes. Virology 212:237–239
    [Google Scholar]
  22. Reddy P. S., Idamakanti I., Derbyshire J. B., Nagy E. 1996; Porcine adenovirus types 1, 2 and 3 have short and simple early E–3 regions. Virus Research 43:99–109
    [Google Scholar]
  23. Reddy P. S., Idamakanti N., Derbyshire J. B., Nagy E. 1997; Characterization of the early region 4 of porcine adenovirus type 3. Virus Genes 15:87–90
    [Google Scholar]
  24. Reddy P. S., Idamakanti N., Song J. Y., Lee J. B., Hyun B. H., Park J. H., Cha S. H., Tikoo S. K., Babiuk L. A. 1998a; Sequence and transcription map analysis of early region-1 of porcine adenovirus type-3. Virus Research (in press)
    [Google Scholar]
  25. Reddy P. S., Idamakanti N., Song J. Y., Lee J. B., Hyun B. H., Park J. H., Cha S. H., Bae Y. T., Tikoo S. K., Babiuk L. A. 1998b; Nucleotide sequence and transcription map of porcine adenovirus type 3. Virology 251: (in press)
    [Google Scholar]
  26. Saito I., Oya Y., Yamamoto K., Yuasa T., Shimojo H. 1985; Construction of nondefective adenovirus type 5 bearing a 2.8-kilobase hepatitis B virus DNA near the right end ofits genome. Journal of Virology 54:711–719
    [Google Scholar]
  27. Sheppard M., Werner W., Tsatas E., McCoy R., Prowse S., Johnson M. 1998; Fowl adenovirus recombinant expressing VP2 of infectious bursal disease virus induces protective immunity against bursal disease. Archives of Virology 143:915–930
    [Google Scholar]
  28. Tuboly T., Nagy E., Derbyshire J. B. 1993; Potential viral vectors for the stimulation of mucosal antibody responses against enteric viral antigens in pigs. Research in Veterinary Science 54:345–350
    [Google Scholar]
  29. Wathen M. W., Wathen L. M. K. 1984; Isolation, characterization, and physical mapping of a pseudorabies virus mutant containing antigenically altered gp50. Journal of Virology 51:57–62
    [Google Scholar]
  30. Xu Z. Z., Hyatt A., Boyle D. B., Both G. W. 1997; Construction of ovine adenovirus recombinants by gene insertion or deletion of related terminal region sequences. Virology 230:62–71
    [Google Scholar]
  31. Zakhartchouk A., Reddy P. S., Baxi M. K., Baca-Estrada M. E., Babiuk L. A., Tikoo S. K. 1998; Construction and characterization of E3 deleted bovine adenovirus type 3 expressing full length and truncated form of bovine herpesvirus glycoprotein gD. Virology 250:220–229
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-3-563
Loading
/content/journal/jgv/10.1099/0022-1317-80-3-563
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error