1887

Abstract

A 45 kDa glycoprotein was purified from the culture media of human cytomegalovirus (HCMV)-infected fibroblasts. N-terminal sequencing revealed that the protein, R27080, is the translation product of the R27080 open reading frame of HCMV. R27080 is highly glycosylated and contains no cysteine or methionine residues. Proteolytic cleavage of R27080 by a furin-like enzyme was analysed in transfected COS-7 cells. R27080 is the first identified viral protein secreted from HCMV-infected cells.

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/content/journal/jgv/10.1099/0022-1317-80-2-437
1999-02-01
2022-05-29
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References

  1. Ahuja S. K., Murphy P. M. 1993; Molecular piracy of mammalian interleukin-8 receptor type B by herpesvirus saimiri. Journal of Biological Chemistry 268:20691–20694
    [Google Scholar]
  2. Barr P. J. 1991; Mammalian subtilisins: the long-sought dibasic processing endoproteases. Cell 66:1–3
    [Google Scholar]
  3. Beck S., Barrell B. G. 1988; Human cytomegalovirus encodes a glycoprotein homologous to MHC class-I antigens. Nature 331:269–272
    [Google Scholar]
  4. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A., Kouzarides T., Martignetti J. A., Preddie E., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. G. 1990; Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology 154:125–169
    [Google Scholar]
  5. Gao J.-L., Murphy P. M. 1994; Human cytomegalovirus open reading frame US28 encodes a functional beta chemokine receptor. Journal of Biological Chemistry 269:28539–28542
    [Google Scholar]
  6. Giri J. G., Ahdieh M., Eisenman J., Shanebeck K., Grabstein K., Kumaki S., Namen A., Park L. S., Cosman D., Anderson D. 1994; Utilization of the and β and γ chains of the IL-2 receptor by the novel cytokine IL-15. EMBO Journal 13:2822–2830
    [Google Scholar]
  7. Fanslow W. C., Anderson D., Grabstein K. H., Clark E. A., Cosman D., Armitage R. J. 1992; Soluble forms of CD40 inhibit biologic responses of B cells. Journal of Immunology 149:655–660
    [Google Scholar]
  8. McMahan C. J., Slack J. L., Mosley B., Cosman D., Lupton S. D., Brunton L. L., Grubin C. E., Wignall J. M., Jenkins N. A., Brannan B. I., Copeland N. G., Huebner K., Croce C. M., Cannizzarro L. A., Benjamin D., Dower S. K., Spriggs M. K., Sims J. E. 1991; Anovel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. EMBO Journal 10:2821–2832
    [Google Scholar]
  9. Neipel F., Albrecht J.-C., Ensser A., Huang Y.-Q., Li J. J., Friedman-Kien A. E., Fleckenstein B. 1997; Human herpesvirus 8 encodes a homolog of interleukin-6. Journal of Virology 71:839–842
    [Google Scholar]
  10. Rawlinson W. D., Barrell B. G. 1993; Spliced transcripts of human cytomegalovirus. Journal of Virology 67:5502–5513
    [Google Scholar]
  11. Ryon J. J., Hayward S. D., MacMahon E. M. E., Mann R. B., Ling Y., Charache P., Phelan J. A., Miller G., Ambinder R. F. 1993; In situ detection of lytic Epstein–Barr virus infection: expression of the Not I early gene and viral interleukin-10 late gene in clinical specimens. Journal of Infectious Diseases 168:345–351
    [Google Scholar]
  12. Spriggs M. K., Armitage R. J., Comeau M. R., Strockbine L., Farrah T., Macduff B., Ulrich D., Alderson M. R., Müllberg J., Cohen J. I. 1996; The extracellular domain of Epstein–Barr virus BZLF2 protein binds to HLA-DR beta chain and inhibits antigen presentation. Journal of Virology 70:5557–5563
    [Google Scholar]
  13. Swaminathan S., Hesselton R., Sullivan J., Kieff E. 1993; Epstein–Barr virus recombinants with specifically mutated BCRF1 genes. Journal of Virology 67:7406–7413
    [Google Scholar]
  14. Yao Z., Fanslow W. C., Seldin M. F., Rousseau A.-M., Painter S. L., Comeau M. R., Cohen J. I., Spriggs M. K. 1995; Herpesvirus saimiri encodes a new cytokine, IL-17, which binds to a novel cytokine receptor. Immunity 3:811–821
    [Google Scholar]
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