1887

Abstract

Human astrovirus (HAst) non-structural polyproteins are encoded in two open reading frames linked in expression by a ribosomal frameshifting event. The first of these (ORF 1a) specifies the serine protease, whilst the second (ORF 1b) encodes the virus RNA-dependent RNA polymerase. The ORF 1a product contains an unusual motif for small RNA viruses which could potentially direct proteins to the cell nucleus. We have expressed part of ORF 1a containing this motif and the whole of ORF 1b separately in recombinant baculovirus and raised specific antisera to each. We now report that expressed proteins from ORF 1a accumulate in the nucleus of both baculovirus-infected insect cells and HAst-infected CaCo-2 cells. In contrast the products of ORF 1b remain predominantly cytoplasmic.

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1999-10-01
2020-04-08
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References

  1. Aroonprasert D., Fagerland J. A., Kelso N. E., Zheng S., Woode G. N.. 1989; Cultivation and partial characterisation of bovine astrovirus. Veterinary Microbiology19:113–125
    [Google Scholar]
  2. Carter M. J.. 1994; Genomic organisation and expression of astroviruses and caliciviruses. Archives of Virology Suppl9:429–439
    [Google Scholar]
  3. Carter M. J., Willcocks M. M.. 1996; The molecular biology of astroviruses. Archives of VirologySuppl. 12:277–286
    [Google Scholar]
  4. Dingwall C., Laskey R. A.. 1986; Protein import into the cell nucleus. Annual Review of Cell Biology2:367–390
    [Google Scholar]
  5. Hart G. W., Holt G. D., Hattiwanger R. S.. 1988; Nuclear and cytoplasmic glycosylation: novel saccharide linkages in unexpected places. Trends in Biochemical Science13:380–384
    [Google Scholar]
  6. Jiang B., Monroe S. S., Koonin E. V., Stine S. E., Glass R. I.. 1993; RNA sequence of astrovirus: distinctive genomic organization and a putative retrovirus-like ribosomal frame-shifting signal that directs the viral replicase synthesis. Proceedings of the National Academy of Sciences USA90:10539–10543
    [Google Scholar]
  7. Lewis T. L., Matsui S. M.. 1996; Astrovirus ribosomal frameshifting in an infection-transfection transient expression system. Journal of Virology70:2869–2875
    [Google Scholar]
  8. Lewis T. L., Greenberg H. B., Herrmann J. E., Smith L. S., Matsui S. M.. 1994; Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein. Journal of Virology68:77–83
    [Google Scholar]
  9. Marczinke B., Bloys A. J., Brown T. D. K., Willcocks M. M., Carter M. J., Brierley I.. 1994; The human astrovirus RNA-dependent RNA polymerase coding region is expressed by ribosomal frameshifting. Journal of Virology68:5588–5595
    [Google Scholar]
  10. Monroe S. S., Carter M. J., Herrmann J. E., Kurtz J. B., Matsui S. M.. 1995; The Astroviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp364–367 Edited by Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Vienna & New York: Springer-Verlag;
    [Google Scholar]
  11. Penman S.. 1966; RNA metabolism in HeLa cell nucleus. Journal of Molecular Biology17:117–130
    [Google Scholar]
  12. Robbins J., Dilworth S. M., Laskey R. A., Dingwall C.. 1991; Two interdependent basic domains in nucleoplasmin nuclear targeting sequence: identification of a class of bipartite nuclear targeting sequences. Cell64:615–623
    [Google Scholar]
  13. Willcocks M. M., Carter M. J.. 1993; Identification and sequence determination of the capsid protein gene of human astrovirus serotype 1. FEMS Microbiology Letters114:1–8
    [Google Scholar]
  14. Willcocks M. M., Carter M. J., Laidler F. R., Madeley C. R.. 1990; Growth and characterisation of human faecal astrovirus in a continuous cell line. Archives of Virology113:73–82
    [Google Scholar]
  15. Willcocks M. M., Brown T. D. K., Madeley C. R., Carter M. J.. 1994; The complete sequence of a human astrovirus. Journal of General Virology75:1785–1788
    [Google Scholar]
  16. Woode G. N., Bridger J. C.. 1978; Isolation of small viruses resembling astroviruses and caliciviruses from acute enteritis of calves. Journal of Medical Microbiology11:441–452
    [Google Scholar]
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