Surface antigens have been detected by immunofluorescence in cells transformed by two oncogenic DNA viruses: polyoma (Irlin, 1967; Malmgren, Takemoto & Carney, 1968) and SV 40 (Tevethia, Katz & Rapp, 1965). This technique provides an easy way to study surface antigens as virus determinants in tumour cells. Surface antigens, or some of them, are probably tumour-specific transplantation antigens. Adenoviruses, another group of oncogenic DNA viruses, induce tumours whose cells are known to possess tumour specific transplantation antigens (Eddy, Grubbs & Young, 1964; Trentin & Bryan, 1966; Sjögren, Minowada & Ankerst, 1967; Berman, 1967), but surface antigen has not until now been detected by immunofluorescence (Hollinshead & Alford, 1969).

In this work I report the observation by immunofluorescence of surface antigen in cells derived from a hamster tumour induced by adenovirus type 12. A modification of Möller's technique (Möller, 1961) was used, as well as Tevethia's technique with fixed cells (Tevethia 1965).


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