1887

Abstract

nucleopolyhedrovirus (AcMNPV) ORF 86, located within the dIII C fragment, potentially encodes a protein which shares sequence similarity with two T4 bacteriophage gene products, RNA ligase and polynucleotide kinase. This AcMNPV gene has been designated but has yet to be assigned a function in virus replication. It has been classified as an immediate early virus gene, since the promoter was active in uninfected insect cells and mRNA transcripts were detectable from 4 to 48 h post-infection and in the presence of cycloheximide or aphidicolin in virus- infected cells. The extremities of the transcript have been mapped by primer extension and 3′ RACE-PCR to positions 18 from the translational start codon and 15 downstream of the stop codon. The function of was investigated by producing a recombinant virus (Acdel86lacZ) with the coding region replaced with that of . This virus replicated normally in ( 21) cells, indicating that / is not essential for propagation in these cells. Virus protein production in Acdel86lacZ-infected 21 cells also appeared to be unaffected, with normal synthesis of the IE-1, GP64, VP39 and polyhedrin proteins. Shut-down of host protein synthesis was not abolished in recombinant infection. When other baculovirus genomes were examined for the presence of / by restriction enzyme digestion and PCR, a deletion was found in AcMNPV 1.2, NPV (GmMNPV) and NPV (NPV), suggesting that in many isolates this gene has either never been acquired or has been lost during genome evolution. This is one of the first baculovirus immediate early genes that appears to be nonessential for virus survival.

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1998-03-01
2022-05-22
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