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Two mutant potato virus X (PVX) movement protein (MP) genes (m12K-Sal and m12K-Kpn) were obtained by inserting specific linkers at the boundary between the N-terminal hydrophobic and putative transmembrane segment, and the central invariant hydrophilic region of the respective 12 kDa, 12K, triple gene block (TGB) protein. Several transgenic potato lines which expressed m12K-Sal or m12K- Kpn to different degrees were resistant to infection by PVX, potato aucuba mosaic potexvirus and the carlaviruses potato virus M and S over a wide range of inoculum concentrations (3-300 μg/ml). However,theywere not resistantto potato virus Y, which lacks a TGB protein. We suggest that the resistance of m12K-Sal and m12K-Kpn transgenic potato lines is MP-derived and not RNA-mediated.
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