1887

Abstract

Hepatitis B virus (HBV) preS1 fused to the GAL4 DNA-binding domain functioned as a transcriptional activation domain in yeast and mammalian cells. The GAL4-preS1 fusion proteins derived from the preSI of all three tested HBV subtypes (, and ) specifically activated the transcription of a or chloramphenicol acetyltransferase reporter gene linked to a GAL4-responsive promoter in transient transfection assays using yeast or HepG2 cells, respectively. Deletion analyses showed that the segments of preSI from residues 21 to 90 and from residues 21 to 56 are sufficient and essential for the activity, respectively. Stable expression of GAL4-preS1 in Chinese hamster ovary cells also produced transactivator activity. These results suggest that preS1 fused to any DNA- binding domain of transcription factors would have transactivation potential.

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1997-05-01
2022-10-07
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