1887

Abstract

Infectious cDNA clones of two strains of tick-borne encephalitis (TBE) virus, i.e. European subtype prototypic strain Neudoerfl and the closely related but more virulent strain Hypr, were constructed. The recombinant constructs consisted of cDNAs stably inserted into the bacterial plasmid pBR322 under the control of T7 promoter elements. The genome of TBE virus strain Neudoerfl was successfully cloned, both as a full-length cDNA and as two partial cDNAs. In the case of strain Hypr, the genome is represented by two cDNA clones corresponding to the 5′ - and 3′ - terminal halves of the genome. Highly infectious RNAs can be produced from the full-length cDNA clone or from the partial clones ligated to form full-length cDNA templates prior to T7 transcription. The biological properties of the recombinant progeny viruses, including virulence characteristics, were indistinguishable from the corresponding parent virus strains. Thus, the described infectious cDNA clones represent a useful and reliable experimental system for the specific mutagenesis of TBE virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-5-1049
1997-05-01
2022-05-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/5/9152422.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-5-1049&mimeType=html&fmt=ahah

References

  1. Chambers T. J., Hahn C. S., Galler R., Rice C. M. 1990; Flavivirus genome organization, expression, and replication. Annual Review of Microbiology 44:649–688
    [Google Scholar]
  2. Dobrikova E. Yu., Pletnev A. G. 1995; A full-size DNA copy of the tick-borne encephalitis virus genome. Part I. Analysis of noncoding 5′- and 3′-regions. Bioorganic Chemistry 21:528–534 in Russian
    [Google Scholar]
  3. Gritsun T. S., Gould E. A. 1995; Infectious transcripts of tick-borne encephalitis virus, generated in days by RT-PCR. Virology 214:611–618
    [Google Scholar]
  4. Guirakhoo F., Heinz F. X., Kunz C. 1989; Epitope model of tick- borne encephalitis virus envelope glycoprotein E: analysis of structural properties, role of carbohydrate side chain, and conformational changes occuring at acidic pH. Virology 169:90–99
    [Google Scholar]
  5. Heinz F. X., Kunz C. 1981; Homogeneity of the structural glycoprotein from European isolates of tick-borne encephalitis virus : comparison with other flaviviruses. Journal of General Virology 57:263–274
    [Google Scholar]
  6. Heinz F. X., Mandl C. W. 1993; The molecular biology of tick-borne encephalitis virus. Acta Pathologica Microbiologica et Immunologica Scandinavica 101:735–745
    [Google Scholar]
  7. Heinz F. X., Tuma W., Guirakhoo F., Kunz C. 1986; A model study of the use of monoclonal antibodies in capture enzyme immunoassays for antigen quantification exploiting the epitope map of tick-borne encephalitis virus. Journal of Biological Standardization 14:133–141
    [Google Scholar]
  8. Heinz F. X., Allison S. L., Stiasny K., Schalich J., Holzmann H., Mandl C. W., Kunz C. 1995; Recombinant and virion-derived soluble and particulate immunogens for vaccination against tick-borne encephalitis. Vaccine 13:1636–1642
    [Google Scholar]
  9. Holzmann H., Stiasny K., York H., Dorner F., Kunz C., Heinz F. X. 1995; Tick-borne encephalitis virus envelope protein E-specific monoclonal antibodies for the study of low pH-induced conformational changes and immature virions. Archives of Virology 140:213–222
    [Google Scholar]
  10. Holzmann H., Stiasny K., Ecker M., Kunz C., Heinz F. X. 1997; Characterization of monoclonal antibody-escape mutants of tick-borne encephalitis virus with reduced neuroinvasiveness in mice. Journal of General Virology 78:31–37
    [Google Scholar]
  11. Kapoor M., Zhang L., Mohan P. M., Padmanabhan R. 1995; Synthesis and characterization of an infectious dengue virus type-2 RNA genome (New Guinea C strain). Gene 162:175–180
    [Google Scholar]
  12. Khromykh A. A., Westaway E. G. 1994; Completion of Kunjin virus RNA sequence and recovery of an infectious RNA transcribed from stably cloned full-length cDNA. Journal of Virology 68:4580–4588
    [Google Scholar]
  13. Kolykhalov A. A., Feinstone S. M., Rice C. M. 1996; Identification of a highly conserved sequence element at the 3′ terminus of hepatitis C virus genome RNA. Journal of Virology 70:3363–3371
    [Google Scholar]
  14. Lai C.-J., Zhao B., Hori H., Bray M. 1991; Infectious RNA transcribed from stably cloned full-length cDNA of dengue type 4 virus. Proceedings of the National Academy of Sciences, USA 88:5139–5143
    [Google Scholar]
  15. Mandl C. W., Heinz F. X., Kunz C. 1988; Sequence of the structural proteins of tick-borne encephalitis virus (Western subtype) and comparative analysis with other flaviviruses. Virology 166:197–205
    [Google Scholar]
  16. Mandl C. W., Heinz F. X., Stöckl E., Kunz C. 1989; Genome sequence of tick-borne encephalitis virus (Western subtype) and comparative analysis of nonstructural proteins with other flaviviruses. Virology 173:291–301
    [Google Scholar]
  17. Mandl C. W., Kunz C., Heinz F. X. 1991; Presence of poly(A) in a flavivirus: significant differences between the 3′ noncoding regions of the genomic RNAs of tick-borne encephalitis virus strains. Journal of Virology 65:4070–4077
    [Google Scholar]
  18. Pletnev A. G., Bray M., Huggins J., Lai C.-J. 1992; Construction and characterization of chimeric tick-borne encephalitis/dengue type 4 viruses. Proceedings of the National Academy of Sciences, USA 89:10532–10536
    [Google Scholar]
  19. Pletnev A. G., Bray M., Lai C.-J. 1993; Chimeric tick-borne encephalitis and dengue type 4 viruses: effects of mutations on neurovirulence in mice. Journal of Virology 67:4956–4963
    [Google Scholar]
  20. Pryor M. J., Gualano R. C., Davidson A. D., Wright P. J. 1996; Site-directed mutagenesis of NS1 using a dengue-2 infectious clone. Xth International Congress of Virology Jerusalem, Israel: 11-16 August 1996 Abstract PW02-28
    [Google Scholar]
  21. Rey F. A., Heinz F. X., Mandl C., Kunz C., Harrison S. C. 1995; The envelope glycoprotein from tick-borne encephalitis virus at 2 Å resolution. Nature 375:291–298
    [Google Scholar]
  22. Rice C. A., Grakoui A., Galler R., Chambers T. J. 1989; Transcription of infectious yellow fever RNA from full-length cDNA templates produced by in vitro ligation. New Biologist 1:285–296
    [Google Scholar]
  23. Sumiyoshi H., Hoke C. H., Trent D. W. 1992; Infectious Japanese encephalitis virus RNA can be synthesized from in vitro-ligated cDNA templates. Journal of Virology 66:5425–5431
    [Google Scholar]
  24. Sumiyoshi H., Tignor G. H., Shope R. E. 1995; Characterization of a highly attenuated Japanese encephalitis virus generated from molecularly cloned cDNA. Journal of Infectious Diseases 171:1144–1151
    [Google Scholar]
  25. Tanaka T., Kato N., Cho M.-J., Sugiyama K., Shimotohno K. 1996; Structure of the 3′ terminus of the hepatitis C virus genome. Journal of Virology 70:3307–3312
    [Google Scholar]
  26. Wallner G., Mandl C. W., Kunz C., Heinz F. X. 1995; The flavivirus 3′-noncoding region: extensive size heterogeneity independent of evolutionary relationships among strains of tick-borne encephalitis virus. Virology 213:169–178
    [Google Scholar]
  27. Wallner G., Mandl C. W., Ecker M., Holzmann H., Stiasny K., Kunz C., Heinz F. X. 1996; Characterization and complete genome sequences of high- and low-virulence variants of tick-borne encephalitis virus. Journal of General Virology 77:1035–1042
    [Google Scholar]
  28. Wengler G., Bradley D. W., Collett M. S., Heinz F. X., Schlesinger R. W., Strauss J. H. 1995; Flaviviridae. In Virus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp. 415–427 Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Edited by Vienna & New York: Springer-Verlag;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-5-1049
Loading
/content/journal/jgv/10.1099/0022-1317-78-5-1049
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error