1887

Abstract

Herpes simplex virus type 1 (HSV-1) immediate early protein IE63, an essential nuclear protein, is pleiotropic in function and, at the post-transcriptional level, inhibits RNA splicing, interacts with cellular splicing small nuclear ribonucleoprotein particles (snRNPs), binds RNA and prevents the nucleocytoplasmic transport of intron-containing mRNAs. Here it is reported that IE63 is a nucleo-cytoplasmic shuttle protein able to travel from snRNP-and RNA-rich nuclear foci to the cytoplasm, where it accumulates during actinomycin D treatment. This newly identified property suggests that IE63 facilitates nuclear export of HSV-1 transcripts, in addition to retaining intron-containing transcripts in the nucleus. The mechanism by which IE63 controls RNA export has yet to be defined.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-12-3327
1997-12-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/12/9400984.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-12-3327&mimeType=html&fmt=ahah

References

  1. Ackermann M., Brown D. K., Periera L., Roizman B. 1984; Characterisation of herpes simplex virus 1 α proteins 0, 4 and 27 with monoclonal antibodies. Journal of Virology 52:108–118
    [Google Scholar]
  2. Brown C. R., Nakamura M. S., Mosca J. D., Hayward G. S., Strauss S. E., Perera L. P. 1995; Herpes simplex virus trans-regulatory protein ICP27 stabilises and binds to 3′ ends of labile mRNA. Journal of Virology 69:7187–7195
    [Google Scholar]
  3. Carmo-Fonseca M., Pepperkok R., Carvalho M. T., Lamond A. I. 1992; Transcription-dependent colocalisation of the U1, U2, U4/U6 and U5 snRNPs in coiled bodies. Journal of Cell Biology 117:1–14
    [Google Scholar]
  4. Dreyfuss G., Matunis M. J., Pinol-Roma S., Burd C. G. 1993; hnRNP proteins and the biogenesis of mRNA. Annual Reviews in Biochemistry 62:289–321
    [Google Scholar]
  5. Du L., Warren S. L. 1997; A functional interaction between the carboxy-terminal domain of RNA polymerase II and pre-mRNA splicing. Journal of Cell Biology 136:5–18
    [Google Scholar]
  6. Everett R. D., Preston C. M., Stow N. D. 1991; Functional and genetic analysis of the role of Vmw110 in herpes simplex virus replication. In The Control of Herpes Simplex Virus Gene Expression pp. 50–76 Wagner E. K. Edited by Boca Raton, Fla: CRC Press;
    [Google Scholar]
  7. Everett R. D., Cross A., Orr A. 1993; A truncated form of HSV-1 immediate early protein Vmw110 is expressed in a cell-type dependent manner. Virology 197:751–756
    [Google Scholar]
  8. Gao M., Knipe D. M. 1991; Potential role for herpes simplex virus ICP8 DNA replication protein in stimulation of late gene expression. Journal of Virology 65:2666–2675
    [Google Scholar]
  9. Habets W. J., Hoet M. H., De Jong B. A. W., Van Der Kemp A., Van Venrooij W. J. 1989; Mapping of B-cell epitopes on small nuclear ribonucleoproteins that react with human autoantibodies as well as with experimentally-induced mouse monoclonal-antibodies. Journal of Immunology 143:2560–2566
    [Google Scholar]
  10. Hardy W. R., Sandri-Goldin R. M. 1994; Herpes simplex virus inhibits host cell splicing and regulatory protein ICP27 is required for this effect. Journal of Virology 68:7790–7799
    [Google Scholar]
  11. Ingram A., Phelan A., Dunlop J., Clements J. B. 1996; Immediate early protein IE63 of herpes simplex virus type 1 binds RNA directly. Journal of General Virology 77:1847–1851
    [Google Scholar]
  12. Kalland K.-H., Szilvay A. M., Brokstad K. A., Saetrevik W., Haukenes G. 1994; The human immunodeficiency virus type 1 Rev protein shuttles between the cytoplasm and nuclear compartments. Molecular and Cellular Biology 14:7436–7444
    [Google Scholar]
  13. McCarthy A. M., McMahan L., Schaffer P. A. 1989; Herpes simplex virus type 1 ICP27 deletion mutants exhibit altered patterns of transcription and are DNA deficient. Journal of Virology 63:18–27
    [Google Scholar]
  14. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., McNab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  15. McGregor F., Phelan A., Dunlop J., Clements J. B. 1996; Regulation of herpes simplex virus poly(A) site usage; the early-late switch and the action of immediate early protein IE63. Journal of Virology 70:1931–1940
    [Google Scholar]
  16. Mears W. E., Rice S. A. 1996; The RGG box motif of the HSV ICP27 protein mediates an RNA binding activity and determines in vivo methylation. Journal of Virology 70:7445–7453
    [Google Scholar]
  17. Mears W. E., Lam V., Rice S. A. 1995; Identification of nuclear and nucleolar localisation signals in the HSV regulatory protein ICP27. Journal of Virology 69:935–947
    [Google Scholar]
  18. Nakielny S., Dreyfuss G. 1996; The hnRNP C proteins contain a nuclear retention sequence that can override nuclear export signals. Journal of Cell Biology 134:1365–1373
    [Google Scholar]
  19. Panagiotidis C. A., Lium E. K., Silverstein S. J. 1997; Physical and functional interactions between herpes simplex virus immediate early proteins ICP4 and ICP27. Journal of Virology 71:1547–1557
    [Google Scholar]
  20. Perera L. P., Kaushal S., Kinchington P. R., Mosca J. D., Hayward G. S., Strauss S. E. 1994; ptional activator that is functionally distinct from that of HSV homolog IC27. Journal of Virology 68:2468–2477
    [Google Scholar]
  21. Phelan A., Carmo-Fonseca M., McLauchlan J., Lamond A. I., Clements J. B. 1993; A herpes simplex virus type-1 immediate early gene product, IE63, regulates small nuclear ribonucleoprotein distribution. Proceedings of the National Academy of Sciences, USA 90:9056–9060
    [Google Scholar]
  22. Phelan A., Dunlop J., Clements J. B. 1996; Herpes simplex virus type 1 protein IE63 affects the nuclear export of virus intron-containing transcripts. Journal of Virology 70:5255–5265
    [Google Scholar]
  23. Phelan A., Dunlop J., Patel A. H., Stow N. D., Clements J. B. 1997; Nuclear sites of herpes simplex virus type 1 DNA replication and transcription colocalise at early times post infection and are largely distinct from RNA processing factors. Journal of Virology 71:1124–1132
    [Google Scholar]
  24. Pinol-Roma S., Dreyfuss G. 1992; Shuttling of pre-mRNA binding proteins between the nucleus and cytoplasm. Nature 355:730–732
    [Google Scholar]
  25. Rice S. A., Lam V., Knipe D. M. 1993; The acidic amino-terminal region of herpes simplex virus type 1 alpha protein ICP27 is required for an essential lytic function. Journal of Virology 67:1778–1787
    [Google Scholar]
  26. Ruhl M., Himmelspath M., Bahr G. M., Hammerschmidt F., Jaksche H., Wolff B., Aschauer H., Farrington G. K., Pivost H., Burck D. A. D., Hauber J. 1993; Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating trans-activation. Journal of Cell Biology 123:1309–1320
    [Google Scholar]
  27. Sandri-Goldin R. M., Hibbard M. K. 1996; The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-Sm antiserum, and the C-terminus appears to be required for this interaction. Journal of Virology 70:108–118
    [Google Scholar]
  28. Spector D. L. 1993; Nuclear organisation of pre-mRNA processing. Current Opinion in Cell Biology 5:442–448
    [Google Scholar]
  29. Wen W., Meinkoth J. L., Tsien R. Y., Taylor S. S. 1995; Identification of a signal for rapid export of proteins from the nucleus. Cell 82:463–473
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-12-3327
Loading
/content/journal/jgv/10.1099/0022-1317-78-12-3327
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error