1887

Abstract

The nucleotide sequences of two S/da-infecting geminiviruses from Honduras were determined. The symptoms of both viruses are identical in but different in . An additional symptom of one virus was yellow vein clearing on infected leaves. Both Sida golden mosaic viruses (SiGMV-Ho and SiGMV- Ho) have bipartite genomes (DNAs A and B). From the SiGMV-Ho-infected plant two different DNA B molecules were isolated and cloned. They differ in length by 24 nucleotides [SiGMV-Ho B (2593 nt) and B (2569 nt)] and at eight nucleotide positions. Both proteins encoded by DNA B(BV1 and BC1) are affected by these substitutions. Computer analysis shows that the bipartite genomes resemble those of other whitefly-transmitted geminiviruses. From homology analyses we conclude that both viruses are closely related but distinct. Comparison with a Sida-infecting virus from Costa Rica (SiGMV-Co) showed that the two viruses from Honduras are more similar to each other than either of them are to SiGMV-Co. Exchange of SiGMV-Ho and SiGMV-Ho genomic components resulted in viable pseudorecombinant viruses. SiGMV-Ho DNA A was able to produce a viable pseudorecombinant with SiGMV-Co DNA B while the reciprocal exchange was not infectious in . SiGMV-Ho^ DNA A and SiGMV-Co DNA B produced a viable pseudorecombinant virus whereas only pseudorecombination of SiGMV-Co DNA A with SiGMV-Ho DNA B, and not with DNA B, was infectious in .

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1997-10-01
2021-10-22
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