@article{mbs:/content/journal/jgv/10.1099/0022-1317-78-10-2675, author = "Frischmuth, Thomas and Engel, Margit and Lauster, Susanne and Jeske, Holger", title = "Nucleotide sequence evidence for the occurrence of three distinct whitefly-transmitted, Sida-infecting bipartite geminiviruses in Central America", journal= "Journal of General Virology", year = "1997", volume = "78", number = "10", pages = "2675-2682", doi = "https://doi.org/10.1099/0022-1317-78-10-2675", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-10-2675", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The nucleotide sequences of two S/da-infecting geminiviruses from Honduras were determined. The symptoms of both viruses are identical in S/da rhomb/fol/a but different in N/cot/ana bentham/ana. An additional symptom of one virus was yellow vein clearing on infected N. bentham/ana leaves. Both Sida golden mosaic viruses (SiGMV-Ho and SiGMV- Hoyv) have bipartite genomes (DNAs A and B). From the SiGMV-Hoyv-infected S. rhombifolia plant two different DNA B molecules were isolated and cloned. They differ in length by 24 nucleotides [SiGMV-Hoyv B1 (2593 nt) and B2 (2569 nt)] and at eight nucleotide positions. Both proteins encoded by DNA B(BV1 and BC1) are affected by these substitutions. Computer analysis shows that the bipartite genomes resemble those of other whitefly-transmitted geminiviruses. From homology analyses we conclude that both viruses are closely related but distinct. Comparison with a Sida-infecting virus from Costa Rica (SiGMV-Co) showed that the two viruses from Honduras are more similar to each other than either of them are to SiGMV-Co. Exchange of SiGMV-Ho and SiGMV-Hoyv genomic components resulted in viable pseudorecombinant viruses. SiGMV-Ho DNA A was able to produce a viable pseudorecombinant with SiGMV-Co DNA B while the reciprocal exchange was not infectious in N. bentham/ana. SiGMV-Ho^ DNA A and SiGMV-Co DNA B produced a viable pseudorecombinant virus whereas only pseudorecombination of SiGMV-Co DNA A with SiGMV-Hoyv DNA B2, and not with DNA B1, was infectious in N. bentham/ana.", }