1887

Abstract

African horsesickness virus serotype 4 (AHSV-4) outer-capsid proteins VP2 or VP2 and VP5, prepared from single or dual recombinant baculovirus expression vectors grown in Sf9 insect cells, were administered in different amounts to horses and the neutralizing antibody responses were measured. Control and vaccinated horses were challenged with virulent AHSV-4 6 months later and monitored post challenge. The results indicated that two inoculations of extracts containing VP2 and VP5, or VP2 alone, in doses of 5 µg VP2 or more per horse, were sufficient to elicit protection against African horsesickness (AHS) disease. The recombinant VP2 protein is a potential candidate vaccine for AHS in horses.

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1996-09-01
2021-10-28
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References

  1. Bradford M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  2. Brown M., Faulkner P. 1977; A plaque assay for nuclear-polyhedrosis viruses using a solid overlay. Journal of General Virology 36:361–364
    [Google Scholar]
  3. Burrage T. G., Trevejo R., Stone-Marschat M., Laegreid W. W. 1993; Neutralizing epitopes of African horsesickness virus serotype 4 are located on VP2. Virology 196:799–803
    [Google Scholar]
  4. Howell P. G. 1962; The isolation and identification of further antigenic types of African horsesickness virus. Onderstepoort Journal of Veterinary Research 29:139–149
    [Google Scholar]
  5. Huismans H., Van Der Walt N. T., Cloete M., Erasmus B. J. 1987; Isolation of capsid protein of bluetongue that induces a protective immune response in sheep. Virology 157:172–179
    [Google Scholar]
  6. Martinez-Torrecuadrada J. L., Iwata H., Venteo A., Casal I., Roy P. 1994; Expression and characterization of the two outer capsid proteins of African horsesickness virus: the role of VP2 in virus neutralization. Virology 202:348–359
    [Google Scholar]
  7. Mertens P. P. C. 1994; Orbiviruses and Coltiviruses. In Encyclopedia of Virology vol 2 pp 941–956 Edited by Webster R. G., Granoff A. San Diego: Academic Press;
    [Google Scholar]
  8. Reid R., van der Meyden C. H., Erasmus B. J., Meyer H., Hamilton A. M. 1992; Encephalitis and chorioretinitis associated with neurotropic African horsesickness virus infection in laboratory workers. Part II. Ophthalmological findings. South African Medical Journal 81:454–459
    [Google Scholar]
  9. Rodriguez M., Hooghuis H., Castano M. 1992; African horse sickness in Spain. Veterinary Microbiology 33:129–142
    [Google Scholar]
  10. Roy P., Urakawa T., van Dijk A. A., Erasmus B. J. 1990; Recombinant virus vaccine for bluetongue disease in sheep. Journal of Virology 64:1998–2003
    [Google Scholar]
  11. Roy P., French T. J., Erasmus B. J. 1992; Protective efficacy of virus-like particles for bluetongue disease. Vaccine 10:28–32
    [Google Scholar]
  12. Roy P., Mertens P. P. C., Casal I. 1994a; African horsesickness virus structure. Comparative Immunology Microbiology and Infectious Diseases 17:243–273
    [Google Scholar]
  13. Roy P., Bishop D. H. L., LeBlois H., Erasmus B. J. 1994b; Long-lasting protection of sheep against bluetongue challenge after vaccination with virus-like particles: evidence for homologous and partial heterologous protection. Vaccine 12:805–811
    [Google Scholar]
  14. Taylor M. B., Van Der Meyden C. H., Erasmus B. J., Reid R., Labuscagne J. H., Dreyer L., Prozesky O. W. 1992; Encephalitis and chorioretinitis associated with neurotropic African horsesickness virus infection in laboratory workers. Part IV. Experimental infection of the vervet monkey (Cercopithecus pygerythrus). South African Medical Journal 81:462–467
    [Google Scholar]
  15. Van Der Meyden C. H., Erasmus B. J., Swanepoel R., Prozesky O. W. 1992; Encephalitis and chorioretinitis associated with neurotropic African horsesickness virus infection in laboratory workers. Part I. Clinical and neurological observations. South African Medical Journal 81:451–454
    [Google Scholar]
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