1887

Abstract

Nucleotide sequences of regions of the envelope () and polymerase () genes of human immunodeficiency virus type 1 (HIV-1) proviral DNA were obtained from sequential blood and autopsy samples from an AIDS patient who had been treated with zidovudine for 9 months. Phylogenetic analyses showed that a reduction in genetic heterogeneity of the regions of viruses present in the proviral blood population occurred during therapy, and this coincided with an increased gene heterogeneity. Differences were observed in different organs obtained for both the and coding regions. The cardiac blood proviral population consisted mainly of variants which possessed sequences containing mutations at position 215 of the gene, associated with drug resistance. By contrast, the brain population consisted entirely of zidovudine sensitive genotypes, and this organ also harboured variants with genetically distinct sequences. The lymph tissues obtained after death held more diverse proviral and populations, containing both zidovudine sensitive and resistant genotypes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-77-5-1071
1996-05-01
2022-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/77/5/JV0770051071.html?itemId=/content/journal/jgv/10.1099/0022-1317-77-5-1071&mimeType=html&fmt=ahah

References

  1. Albert J., Wahlberg J., Lundeberg J., Cox S., Sandstrom E., Wahren B., Uhlen M. 1992; Persistence of azidothymidine-resistant human immunodeficiency virus type 1 RNA genotypes in post-treatment sera. Journal of Virology 66:5627–5630
    [Google Scholar]
  2. Balfe P., Simmonds P., Ludlam C. A., Bishop J. O., Leigh-Brown A. J. 1990; Concurrent evolution of human immunodeficiency virus type 1 in patients infected from the same source: rate of sequence change and low frequency of inactivating mutations. Journal of Virology 64:6221–6233
    [Google Scholar]
  3. Ball J. K., Desselberger U. 1992; Incorporation of singlestranded DNA binding protein early in polymerase chain reaction product sequencing reactions prevents enzyme pausing. Analytical Biochemistry 207:349–351
    [Google Scholar]
  4. Ball J. K., Holmes E. C., Whitwell H., Desselberger U. 1994; Genomic variation of human immunodeficiency virus type 1 (HIV-1): molecular analyses of HIV-1 in sequential blood samples and various organs obtained at autopsy. Journal of General Virology 75:867–879
    [Google Scholar]
  5. Balzarini J., Pauwels R., Baba M., Herdewijn P., DeClercq E., Broder S., Johns D. G. 1988; The in vitro and in vivo antiretrovirus activity, and intracellular metabolism of 3′-azido-2′, 3′-dideoxycytidine are highly dependent on the cell species. Biochemical Pharmacology 37:897–903
    [Google Scholar]
  6. Crowe S. M., McGrath M. S., Elbeik T., Kirihara J., Mills J. 1989; Comparative assessment of anti-retrovirals in human monocyte-macrophage and lymphoid cell lines acutely and chronically infected with the human immunodeficiency virus. Journal of Medical Virology 29:176–180
    [Google Scholar]
  7. Delassus S., Cheynier R., Wain-Hobson S. 1992; Nonhomogeneous distribution of human immunodeficiency virus type 1 proviruses in the spleen. Journal of Virology 66:5642–5645
    [Google Scholar]
  8. Di Stefano M., Norkrans G., Chiodi F., Hagberg L., Nielsen C., Svennerholm B. 1993; Zidovudine-resistant variants of HIV-1 in brain. Lancet 342:865
    [Google Scholar]
  9. Donaldson Y. K., Bell J. E., Ironside J. W., Brettle R. P., Robertson J. R., Busuttil A., Simmonds P. 1994; Redistribution of HIV outside the lymphoid system with onset of AIDS. Lancet 343:382–385
    [Google Scholar]
  10. Embretson J., Zupanic M., Ribas J. L., Burke A., Racz P., Tenner-Racz K., Haase A. T. 1993; Massive covert infection of helper T lymphocytes and macrophages by HIV during the incubation period of AIDS. Nature 362:359–362
    [Google Scholar]
  11. Epstein L. G., Kuiken C., Blumberg B. M., Hartman S., Sharer L. R., Clement M., Goudsmit J. 1991; HIV-1 V3 domain variation in brain and spleen of children with AIDS: tissue-specific evolution within host-determined quasispecies. Virology 180:583–590
    [Google Scholar]
  12. Fouchier R. A. M., Groenink M., Kootstra N. A., Tersmette M., Huisman H. G., Miedema F., Schuitemaker H. 1992; Phenotype-associated sequence variation in the third variable domain of the human immunodeficiency virus type 1 gpl20 molecule. Journal of Virology 66:3183–3187
    [Google Scholar]
  13. Gartner S., Markovits P., Markovitz D. M., Kaplan M. H., Gallo R. C., Popovic M. 1986; The role of mononuclear phagocytes in HTLV-III/LAV infection. Science 233:215–219
    [Google Scholar]
  14. Groenink M., Fouchier R. A. M., Broerson S., Baker C. H., Koot M., van’t Woot A. B., Huisman H. G., Miedema F., Tersmette M., Schuitemaker H. 1993; Relation of phenotype evolution of HIV-1 to envelope V2 configuration. Science 260:1513–1516
    [Google Scholar]
  15. Gu Z., Gao Q., Faust E. A., Wainberg M. A. 1995; Possible involvement of cell fusion and viral recombination in generation of human immunodeficiency virus variants that display dual resistance to AZT and 3TC. Journal of General Virology 76:2601–2605
    [Google Scholar]
  16. Hao Z., Cooney D. A., Hartman N. R., Perno C. F., Fridland A., DeVico A. L., Sarngadharan M. G., Broder S., Johns D. G. 1988; Factors determining the activity of 2′, 3′-dideoxynucleosides in suppressing human immunodeficiency virus in vitro . Molecular Pharmacology 34:431–435
    [Google Scholar]
  17. Ho D. D., Neumann A. U., Perelson A. S., Chen W., Leonard J.M., Markowitz M. 1995; Rapid turnover of plasma virions and CD4 lymphocytes in HIV-1 infection. Nature 373:123–126
    [Google Scholar]
  18. Holmes E. C., Zhang L. Q., Simmonds P., Ludlam C. A., Leigh Brown A. J. 1992; Convergent and divergent evolution in the surface envelope glycoprotein of human immunodeficiency virus type 1 within a single infected patient. Proceedings of the National Academy of Sciences, USA 89:4835–4839
    [Google Scholar]
  19. Javaherian K., Langlois A. J., McDanal C., Ross K. L., Eckler L. L., Jellis C. L., Profy A. T., Rusche J. R., Bolognesi D. P., Putney S. D., Matthews T. J. 1989; Principal neutralizing domain of the human immunodeficiency virus type 1 envelope protein. Proceedings of the National Academy of Sciences, USA 86:6768–6772
    [Google Scholar]
  20. Kayman S. C., Wu Z., Revesz K., Chen H., Kopelman R., Pinter A. 1994; Presentation of native epitopes in the V1/V2 and V3 regions of human immunodeficiency virus type 1 gpl20 by fusion glycoproteins containing isolated gpl20 domains. Journal of Virology 68:400–410
    [Google Scholar]
  21. Kimura M. A. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120
    [Google Scholar]
  22. Klecker R. W., Collins J. M., Yarchoan R. W., Thomas R., Jenkins J. F., Broder S., Myers C. E. 1987; Plasma and cerobrospinal fluid kinetics of 3′-azido-3′-deoxythimidine: a novel pyrimidine analogue with potential application for the treatment of patients with AIDS and related diseases. Clinical Pharmacology and Therapy 41:407–412
    [Google Scholar]
  23. Larder B. A., Kemp S. D. 1989; Multiple mutations in HIV-1 reverse transcriptase confer high-level resistance to zidovudine (AZT). Science 246:1155–1158
    [Google Scholar]
  24. Larder B. A. 1994; Interactions between drug resistance mutations in human immunodeficiency virus type 1 reverse transcriptase. Journal of General Virology 75:951–957
    [Google Scholar]
  25. LI Y., Luo L., Rasool N., Kang C. Y. 1993; Glycosylation is necessary for the correct folding of human immunodeficiency virus gp120 in CD4 binding. Journal of Virology 67:584–588
    [Google Scholar]
  26. Lupia R. H., Ferencz N., Lertora J. J. L., Agrawal S. K., George W. J., Agrawal K. C. 1993; Comparative pharmacokinetics of two prodrugs of zidovudine in rabbits: enhanced levels of zidovudine in brain tissue. Antimicrobial Agents and Chemotherapy 37:818–824
    [Google Scholar]
  27. McKeating J. A., Bennett J., Zolla-Pazner S., Schutten M., Ashelford S., Leigh Brown A. J., Balfe P. 1993; Resistance of a human serum-selected human immunodeficiency virus type 1 escape mutant to neutralization by CD4 binding site monoclonal antibodies is conferred by a single amino acid change in gpl20. Journal of Virology 67:5216–5255
    [Google Scholar]
  28. Meyerhans A., Cheynier R., Albert J., Seth M., Kwok S., Sninsky J., Mofeldt-Manson L., Åsjö B., Wain-Hobson S. 1989; Temporal fluctuations in HIV quasispecies in vivo are not reflected by sequential HIV isolations. Cell 58:901–910
    [Google Scholar]
  29. Myers G., Korber B., Berzofsky J. A., Smith T. F., Pavlakis G.N. 1994 Human Retroviruses and AIDS Los Alamos: Los Alamos National Laboratory;
    [Google Scholar]
  30. Najera I., Holguin A., Quinones-Mateu M. E., Munoz-Fernandez M. A., Najera R., Lopez-Galindez C., Domingo E. 1995; pol gene quasispecies of human immunodeficiency virus: mutations associated with drug resistance in virus from patients undergoing no drug therapy. Journal of Virology 69:23–31
    [Google Scholar]
  31. Nowak M. A., Bonhoeffer S., Loveday C., Balfe P., Semple M., Kaye S., Tenant-Flowers M., Tedder R. 1995; HIV results in the frame: results confirmed. Nature 375:193
    [Google Scholar]
  32. Oram J. D., Downing R. G., Roff M., Serwankambo N., Clegg J. C. S., Featherstone A. S. R., Booth J. C. 1991; Sequence analysis of the V3 loop regions of the env genes of Ugandan human immunodeficiency proviruses. AIDS Research and Human Retroviruses 7:605–614
    [Google Scholar]
  33. Pantaleo G., Graziosi C., Demarest J. F., Butini L., Montroni M., Fox C. H., Orensiein J. M., Kotler D. P., Fauci A. S. 1993; HIV infection is active and progressive in lymphoid tissue during the clinically latent stage of disease. Nature 362:355–358
    [Google Scholar]
  34. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing evolutionary trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  35. Sharp P. M., Robertson D. L., Gao F. L., Hahn B. H. 1994; Origins and diversity of human immunodeficiency viruses. AIDS 8:S27–S42
    [Google Scholar]
  36. Sheehy N., Desselberger U. 1993; Sequence analysis of reverse transcriptase genes of zidovudine (AZT)-resistant and -sensitive human immunodeficiency virus type 1 strains. Journal of General Virology 74:223–228
    [Google Scholar]
  37. Simmonds P., Balfe P., Peutherer J. F., Ludlam C. A., Bishop J. O., Leigh-Brown A. J. 1990a; Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy number. Journal of Virology 64:864–872
    [Google Scholar]
  38. Simmonds P., Balfe P., Ludlam C. A., Bishop J. O., Leigh Brown A. J. 1990b; Analysis of sequence diversity in hypervariable regions of the external glycoprotein of the human immunodeficiency virus type 1. Journal of Virology 64:5840–5850
    [Google Scholar]
  39. Simmonds P., Zhang L. Q., McOmish F., Balfe P., Ludlam C. A., Leigh-Brown A. J. 1991; Discontinuous sequence change of human immunodeficiency virus (HIV) type 1 env sequences in plasma viral and lymphocyte-associated proviral populations in vivo: implications for models of HIV pathogenesis. Journal of Virology 65:6266–6276
    [Google Scholar]
  40. Takahashi H., Merli S., Putney S. D., Houghten R., Moss B., Germain R. N., Berzofsky J. A. 1989; A single amino acid interchange yields reciprocal CTL specificities for HIV-1 gpl60. Science 246:118–121
    [Google Scholar]
  41. Takeuchi Y., Akutsu M., Murayama K., Shimizu N., Hoshino H. 1991; Host range mutant of human immunodeficiency virus type 1: modification of cell tropism by a single point mutation at the neutralization epitope in the env gene. Journal of Virology 65:1710–1718
    [Google Scholar]
  42. Terasaki T., Pardridge W. M. 1988; Restricted transport of 3′-azido-3′-deoxythimidine and dideoxynucleosides through the blood–brain barrier. Journal of Infectious Diseases 158:630–632
    [Google Scholar]
  43. Thali M., MacArthur C., Furman C., Cavacini L., Posner M., Robinson J., Sodroski J. 1994; Discontinuous, conserved neutralization epitopes overlapping the CD4-binding region of human immunodeficiency virus type 1 gpl20 envelope glycoprotein. Journal of Virology 68:674–680
    [Google Scholar]
  44. Tsang T. C., Bentley D. R. 1989; An improved method using Sequenase that is independent of template concentration. Nucleic Acids Research 16:6238
    [Google Scholar]
  45. Vartanian J. P., Meyerhans A., Asjo B., Wain-Hobson S. 1991; Selection, recombination and G→A hypermutation of human immunodeficiency virus type 1 genomes. Journal of Virology 65:1779–1788
    [Google Scholar]
  46. Westervelt P., Trowbridge D. B., Epstein L. G., Blumberg B. M., Li Y., Hahn B. H., Shaw G. M., Price R. W., Ratner L. 1992; Macrophage-tropism determinants of HIV-1 in vivo . Journal of Virology 66:2577–2582
    [Google Scholar]
  47. Wei X., Ghosh S. K., Taylor M. E., Johnson V. A., Emini E. A., Deutsch P., Lifson J. D., Bonhoeffer S., Nowak M. A., Hahn B. H., Saag M. S., Shaw G. M. 1995; Viral dynamics in human immunodeficiency virus type 1 infection. Nature 373:117–122
    [Google Scholar]
  48. Wolinsky S. M., Wlke C. M., Korber B. T. M., Hutto C., Parks W. P., Rosenblum L. L., Kunstman K. J., Furtado M. R., Munoz J. L. 1992; Selective transmission of human immunodeficiency virus type 1 variants from mothers to infants. Science 255:1134–1137
    [Google Scholar]
  49. Zhang L. Q., Simmonds P., Ludlam C. A., Leigh Brown A. J. 1991; Detection, quantification and sequencing of HIV-1 from the plasma of seropositive individuals and from Factor VIII concentrates. AIDS 5:675–681
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-77-5-1071
Loading
/content/journal/jgv/10.1099/0022-1317-77-5-1071
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error