1887

Abstract

A long-term cell line (SHK-1) supporting replication of the causal virus of infectious salmon anaemia (ISA) has been established. The cell line was developed from a culture of Atlantic salmon ( L.) head kidney cells. CPE was observed in SHK-1 cells 12–14 days after inoculation with ISA-infective tissue material. The time for CPE to develop decreased after repeated passages of medium from infected cell cultures to new cultures. Transmission trials demonstrated that Atlantic salmon parr developed ISA after intraperitoneal injection of preparations made from infected cells and growth medium. The ISA infectivity of the cell preparations increased with incubation time of inoculated cells. Cell cultures in a second passage were found to have a higher infectivity than the primary inoculated cultures. Virus particles with a diameter of approximately 100–120 nm, and which contained an external envelope and granules were seen in electron micrographs of thin sections of infected cells. Most of the virus particles were located extracellularly close to the cell surface, and in some cases, a connection between virus and plasma membrane could be observed. This indicates that virus particles were released by budding. Enveloped virus particles of 45–140 nm in diameter were seen in abundance in electron micrographs of a negatively stained purified virus preparation. Large, highly pleomorphic particles up to 700 nm in the longest dimension were occasionally observed in unpurified preparations. The evidence is therefore strong that the virus isolated in SHK-1 cells is the aetiological agent of ISA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-6-1353
1995-06-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/6/JV0760061353.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-6-1353&mimeType=html&fmt=ahah

References

  1. Christie K. E., Hjeltnes B., Lorens J. B., Namork E., Nerland A. H. 1991; A search for specific gene sequences from the etiological agent of infectious salmon anemia. In Proceedings of the Second International Symposium on Viruses of Lower Vertebrates July 29-31, pp 117–124 Oregon State University; Corvallis, Oreg., USA:
    [Google Scholar]
  2. Dannevig B. H., Falk K. 1994; Atlantic salmon, Salmo salar L., develop infectious salmon anaemia (ISA) after inoculation with in vitro infected leucocytes. Journal of Fish Diseases 17:183–187
    [Google Scholar]
  3. Dannevig B. H., Falk K., Krogsrud J. 1993; Leucocytes from Atlantic salmon, Salmo salar L., experimentally infected with infectious salmon anaemia (ISA) exhibit an impaired response to mitogens. Journal of Fish Diseases 16:351–359
    [Google Scholar]
  4. Dannevig B. H., Falk K., Skjerve E. 1994a; Infectivity of internal tissues of Atlantic salmon, Salmo salar L., experimentally infected with the aetiological agent of infectious salmon anaemia (ISA). Journal of Fish Diseases 17:613–622
    [Google Scholar]
  5. Dannevig B. H., Lauve A., Press C. McL., Landsverk T. 1994b; Receptor-mediated endocytosis and phagocytosis by rainbow trout head kidney cells. Fish & Shellfish Immunology 4:3–18
    [Google Scholar]
  6. Diago M. L., López-Fierro M. P., Razqujn B., Villena A. 1993; Long-term myelopoietic cultures from the renal hematopoietic tissue of the rainbow trout, Oncorhynchus mykiss W.: phenotypic characterization of the stromal cells. Experimental Hematology 21:1277–1287
    [Google Scholar]
  7. Evensen Ø., Thorud K. E., Olsen Y. A. 1991; A morphological study of the gross and light microscopic lesions of infectious anaemia in Atlantic salmon (Salmo salar). Research in Veterinary Science 51:215–222
    [Google Scholar]
  8. Hovland T., Nylund A., Watanabe K., Endresen C. 1994; Observation of infectious salmon anaemia virus in Atlantic salmon, Salmo salar L. Journal of Fish Diseases 17:291–296
    [Google Scholar]
  9. Krogsrud J., Hastein T., Rønningen K. 1989; Infectious pancreatic necrosis virus in Norwegian fish farms. In Viruses of Lower Vertebrates pp 284–291 Edited by Ahne W., Kurstak E. Berlin: Springer-Verlag;
    [Google Scholar]
  10. Madeley C. R. 1972 Virus Morphology Edinburgh: Churchill Livingstone;
    [Google Scholar]
  11. Melby H. P., Krogsrud J., Håstein T., Stenwig H. 1991; All commercial Atlantic salmon seawater farms in Norway harbour carriers of infectious pancreatic necrosis virus (IPNV). In Proceedings of the Second International Symposium on Viruses of Lower Vertebrates July 29–31, pp 211–217 Oregon State University; Corvallis, Oreg., USA:
    [Google Scholar]
  12. Smedsrud T., Dannevig B. H., Tolleshaug H., Berg T. 1984; Endocytosis of a mannose-terminated glycoprotein and formaldehyde-treated human serum albumin in liver and kidney cells from fish (Salmo alpinus L.). Developmental and Comparative Immunology 8:579–588
    [Google Scholar]
  13. Thorud K. E. 1991 Infectious salmon anaemia Thesis for the degree of Doctor scientiarum; Norwegian College of Veterinary Medicine, Oslo, Norway:
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-76-6-1353
Loading
/content/journal/jgv/10.1099/0022-1317-76-6-1353
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error