1887

Abstract

Restriction endonuclease analysis of the DNA extracted orf virus strain NZ2, which had been serially passaged in primary bovine testis cells, revealed a population of variants that had over-grown the wild-type virus. At least three distinct mutant forms were identified in which the right end of the genome had been duplicated and translocated to the left end, accompanied by deletions of sequences at the left end. Sequencing of a single variant isolated from the heterogeneous population revealed that recombination had occurred between non-homologous sequences. In this case, 6.6 kb of DNA at the left end of the genome had been replaced by 19.3 kb from the right end. The transposition resulted in the deletion at the left end of 3.3 kb of DNA encoding three genes and the terminal sequence of a fourth gene. The three genes completely deleted were a homologue of dUTPase, a gene that encodes a protein containing ankyrin-like repeats and a homologue of the 5K gene of the vaccinia virus WR strain. Experimental inoculation of sheep showed that the genes are also non-essential , but that the size of the lesion was reduced, compared with that induced by the wild-type, and resolved more rapidly.

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1995-12-01
2022-05-28
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References

  1. Andrews B. J., Herskowitz I. 1989; The yeast SW14 protein contains a motif present in developmental regulators and is part of a complex involved in cell-cycle dependent transcription. Nature 342:830–833
    [Google Scholar]
  2. Balassu T. C., Robinson A. J. 1987; Orf virus replication in bovine testis cells: kinetics of viral DNA, polypeptide, and infectious virus production and analysis of virion polypeptides. Archives of Virology 97:267–281
    [Google Scholar]
  3. Breeden L., Nasmyth K. 1987; Similarity between cell-cycle genes of budding yeast and fission and the notch gene of Drosophila. Nature 329:651–654
    [Google Scholar]
  4. Buller R. M., Palumbo G. J. 1991; Poxvirus pathogenesis. Microbiological Reviews 55:80–122
    [Google Scholar]
  5. Davis L. H., Bennet V. 1990; Mapping the binding sites of human erythrocyte ankyrin for the anion exchanger and spectrin. Journal of Biological Chemistry 265:10589–10596
    [Google Scholar]
  6. Esposito J. J., Cabradilla C. D., Nakano J. H. 1981; Intragenomic sequence transposition in monkeypox virus. Virology 109:231–243
    [Google Scholar]
  7. Fleming S. B., Fraser K. M., Mercer A. A., Robinson A. J. 1991; Vaccinia virus-like early transcriptional control sequences flank an early gene in orf virus. Gene 97:207–212
    [Google Scholar]
  8. Fleming S. B., Mercer A. A., Fraser K. M., Lyttle D. J., Robinson A. J. 1992; In vivo recognition of orf virus early transcriptional promoters in a vaccinia virus recombinant. Virology 187:464–471
    [Google Scholar]
  9. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. (editors) 1991; Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2
    [Google Scholar]
  10. Franzosos G., Bours V., Park S., Tomita-Yamaguchi M., Kelly K., Siebelist U. 1992; The candidate oncoprotein bcl- 3 is an antagonist of p50/NF-KB-mediated inhibition. Nature 359:339–342
    [Google Scholar]
  11. Fraser K. M., Hill D. F., Mercer A. A., Robinson A. J. 1990; Sequence analysis of the inverted terminal repetition in the genome of the parapoxvirus, orf virus. Virology 176:379–389
    [Google Scholar]
  12. Gillard S., Spehner D., Drillien R., Kirn A. 1986; Localization and sequence of a vaccinia virus gene required for multiplication in human cells. Proceedings of the National Academy of Sciences, USA 83:5573–5577
    [Google Scholar]
  13. Goebel S. J., Johnson G. P., Perkus M. E., Davis S. W., Winslow J. P., Paoletti E. 1990; The complete DNA sequence of vaccinia virus. Virology 179:247–266
    [Google Scholar]
  14. Hatada E. N., Nieters A., Wulczyn G. F., Naumann M., Meyer R., Nucifora G., McKeithan T. W., Scheidereit C. 1992; The ankyrin repeat domains of nf-kb precursor pl05 and the protooncogene bcl- 3 act as specific inhibitors of nf-kb DNA binding. Proceedings of the National Academy of Sciences, USA 89:2489–2493
    [Google Scholar]
  15. Howard S. T., Chan Y. S., Smith G. L. 1991; Vaccinia virus homologues of the shope fibroma virus terminal inverted repeat proteins and a discontinuous ORF related to the tumour necrosis factor receptor family. Virology 180:633–647
    [Google Scholar]
  16. Kotwal G. J., Moss B. 1988; Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant. Virology 167:524–537
    [Google Scholar]
  17. Lemarco K., Thompson C. T., Byers B. P., Walton E. M., McKnight S. L. 1991; Identification of ets- and notch-related subunits in GA binding protein. Science 253:789–792
    [Google Scholar]
  18. Lyttle D. J., Fraser K. M., Fleming S. B., Mercer A. A., Robinson A. J. 1994; Homologs of vascular endothelial growth factor are encoded by the poxvirus orf virus. Journal of Virology 68:84–92
    [Google Scholar]
  19. McGeoch D. J. 1990; Protein sequence comparisons show that the pseudoproteases encoded by poxviruses and certain retroviruses belong to the deoxyuridine triphosphatase family. Nucleic Acids Research 18:4105–4110
    [Google Scholar]
  20. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  21. Massung R. F., Esposito J., Liu L., Qi J., Utterback T. R., Knight J. C., Aubin L., Yuran E. T., Parsons J. M., Loparev V. N., Selivanov N. A., Cavallaro K. F., Kerlavage A. R., Mahy B. W. J., Venter J. C. 1993; Potential virulence determinants in terminal regions of variola smallpox virus genome. Nature 366:748–751
    [Google Scholar]
  22. Massung R. F., Liu L., Qi J., Knight J. C., Yuran T. E., Kerlavage A. R., Parsons J. M., Venter J. C., Esposito J. J. 1994; Analysis of the complete genome of smallpox variola major virus strain Bangladesh-1975. Virology 201:215–240
    [Google Scholar]
  23. Mercer A. A., Fraser K., Barns G., Robinson A. J. 1987; The structure and cloning of orf virus DNA. Virology 157:1–12
    [Google Scholar]
  24. Mercer A. A., Fraser K., Stockwell P. A., Robinson A. J. 1989; A homologue of retroviral pseudoproteases in the para-poxvirus, orf virus. Virology 172:665–668
    [Google Scholar]
  25. Moyer R. W., Rothe C. T. 1980; The white pock mutants of rabbit poxvirus. I. Spontaneous host range mutants contain deletions. Virology 102:119–132
    [Google Scholar]
  26. Moyer R. W., Graves R. L., Rothe C. T. 1980; The white pock (µ) mutants of rabbit poxvirus. III. Terminal DNA sequence duplication and transposition in rabbit poxvirus. Cell 22:545–553
    [Google Scholar]
  27. Pickup D. J., Ink B. S., Parsons B. L., Hu W., Joklik W. K. 1984; Spontaneous deletions and duplications of sequences in the genome of cowpox virus. Proceedings of the National Academy of Sciences, USA 81:6817–6821
    [Google Scholar]
  28. Pickup D. J. 1994; Poxviral modifiers of cytokine response to infection. Infectious Agents and Disease 3:116–127
    [Google Scholar]
  29. Pyles R. B., Sawtell N. M., Thompson R. L. 1992; Herpes simplex virus type 1 dUTPase mutants are attenuated for neuro-virulence, neuroinvasivness, and reactivation from latency. Journal of Virology 66:6706–6713
    [Google Scholar]
  30. Robinson A. J., Ellis G., Balassu T. 1982; The genome of orf virus: restriction endonuclease analysis of viral DNA isolated from lesions of orf virus in sheep. Archives of Virology 71:43–55
    [Google Scholar]
  31. Robinson A. J., Barns G., Fraser K., Carpenter E., Mercer A. A. 1987; Conservation and variation of orf virus genomes. Virology 157:13–23
    [Google Scholar]
  32. Smith G. L. 1993; Vaccinia virus glycoproteins and immune evasion. Journal of General Virology 74:1725–1740
    [Google Scholar]
  33. Spehner D., Gillard S., Drillien R., Kirn A. 1988; A cowpox virus gene required for multiplication in Chinese hamster ovary cells. Journal of Virology 62:1297–1304
    [Google Scholar]
  34. Sullivan J. T. 1994 Analysis of a region of the orf virus genome PhD thesis, Department of Microbiology, University of Otago; New Zealand.:
    [Google Scholar]
  35. Sullivan J. T., Mercer A. A., Fleming S. B., Robinson A. J. 1994; Identification and characterization of an orf virus homologue of the vaccinia virus gene encoding the major envelope antigen p37K. Virology 202:968–973
    [Google Scholar]
  36. Sullivan J. T., Fraser K., Fleming S. B., Robinson A. J., Mercer A. A. 1995; Sequence and transcriptional analysis of an orf virus gene encoding ankyrin-like repeat sequences. Virus Genes 9:277–282
    [Google Scholar]
  37. Thompson C. T., Brown T. A., McKnight S. L. 1991; Convergence of ets- and notch-related structural motifs in a heteromeric DNA binding complex. Science 53:762–768
    [Google Scholar]
  38. Threadgill D. S., Steagall W. K., Flaherty M. T., Fuller F. J., Perry S. T., Rushlow K. E., Le Grice S. F. J., Payne S. L. 1993; Characterization of equine infectious anaemia virus dUTPase: growth properties of a dUTPase-deficient mutant. Journal of Virology 67:2592–2600
    [Google Scholar]
  39. Turner P. C., Moyer R. W. 1990; The molecular pathogenesis of poxviruses. Current Topics in Microbiology and Immunology 163:125–151
    [Google Scholar]
  40. Wulczyn G. F., Naumann M., Scheidereit C. 1992; Candidate proto-oncogen bcl- 3 encodes a sub-unit specific inhibitor of transcription factor nf-kb. Nature 358:597–599
    [Google Scholar]
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