1887

Abstract

The ability of rinderpest virus (RPV) to replicate in adherent peripheral blood monocytes and monocyte-derived macrophages under non-stimulation conditions was investigated. When flow cytometry analysis on bovine peripheral blood mononuclear cells (PBMC) was performed, monocytic cells were seen to be targets for infection by the cell culture-attenuated RBOK vaccine strain of RPV. Viral glycoprotein (H) and nucleoprotein (N) expression in adherent blood monocytes and monocyte-derived macrophages was compared with the infection in Vero cells, in which a productive infection typical of morbilliviruses is obtained. In both cell types, the infection was m.o.i.-dependent, but the rate of viral protein accumulation was slower in monocytes/macrophages. Double-labelling experiments with monoclonal antibodies against RPV and the myeloid marker CD14 confirmed that the infected blood adherent cells were monocytes and macrophages. Productive infection of monocytes was confirmed by progeny virus titration. Permissiveness to infection was not dependent on macrophage differentiation: maturation of monocytes to macrophages before infection, did not increase the susceptibility of these cells to RPV infection. With the virulent Saudi RPV isolate, similar results were obtained, although the Saudi virus apparently had a higher rate of replication compared to the attenuated virus. These observations demonstrate clearly that bovine blood monocytes and monocyte-derived macrophages serve as hosts for a relatively slow but productive infection by rinderpest virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-76-11-2779
1995-11-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/76/11/JV0760112779.html?itemId=/content/journal/jgv/10.1099/0022-1317-76-11-2779&mimeType=html&fmt=ahah

References

  1. Anderson J., Mackay J. A. 1994; The detection of antibodies against peste des petits ruminants virus in cattle, sheep and goats and the possible implications to rinderpest control programs. Epidemiology and Infection 112:225–231
    [Google Scholar]
  2. Appel M. J. G., Shek W. R., Summers B. A. 1982; Lymphocyte-mediated immune cytotoxicity in dogs infected with virulent canine distemper virus. Infection and Immunity 37:592–600
    [Google Scholar]
  3. Auger M. J., Ross J. A. 1992; The biology of the macrophage. In The Macrophage pp 27–31 Edited by Lewis C. E., McGee J. O. D. Oxford: IRL Press;
    [Google Scholar]
  4. Davis W. C., Marusic S., Lewin H. A., Splitter G. A., Perryman L. E., McGuire T. C., Gorham J. R. 1987; The development and analysis of species specific and cross reactive monoclonal antibodies to leukocyte differentiation antigens and antigens of the major histocompatibility complex for use in the study of the immune system in cattle and other species. Veterinary Immunology and Immunopathology 15:337–376
    [Google Scholar]
  5. Diallo A., Barrett T., Lefèvre P. C., Taylor W. P. 1987; Comparison of proteins induced in cells infected with rinderpest and peste des petits ruminants viruses. Journal of General Virology 68:2033–2038
    [Google Scholar]
  6. Dransfield I., Corcoran D., Patridge L. J., Hogg N., Burton D. R. 1988; Comparison of human monocytes isolated by elutriation and adherence suggests that heterogeneity may reflect a continuum of maturation/activation states. Immunology 63:491–198
    [Google Scholar]
  7. Drastini Y., Kibenge F. S. B., McKenna P. K., Lopez A. 1992; Comparison of eight different procedures for harvesting avian reoviruses grown in Vero cells. Journal of Virological Methods 39:269–278
    [Google Scholar]
  8. Esolen L. M., Ward B. J., Moesch T. R., Griffin D. E. 1993; Infection of monocytes during measles. Journal of Infectious Diseases 168:47–52
    [Google Scholar]
  9. Gendelman H. E., Narayan O., Kennedy-Stoskopf S., Kennedy P. G. E., Ghotbi Z., Clemens J. E., Stanley J., Pezeshkpour G. 1986; Tropism of sheep lentiviruses for monocytes: susceptibility to infection and virus gene expression increase during maturation of monocytes to macrophages. Journal of Virology 58:67–74
    [Google Scholar]
  10. Hyypiӓ T., Korkiamӓki P., Vainionpӓӓ R. 1985; Replication of measles virus in human lymphocytes. Journal of Experimental Medicine 161:1261–1271
    [Google Scholar]
  11. Labalette-Houache M., Torpier G., Capron A., Dessaint J. P. 1991; Improved permeabilization procedure for flow cytometry detection of internal antigens: analysis of interleukin-2 production. Journal of Immunological Methods 138:143–153
    [Google Scholar]
  12. Libeau G., Lefèvre P. C. 1990; Comparison of rinderpest and peste des petits ruminants viruses using anti-nucleoprotein monoclonal antibodies. Veterinary Microbiology 25:1–16
    [Google Scholar]
  13. Liess B., Plowright W. 1963; The propagation and growth characteristics of rinderpest virus in HeLa cells. Archiv fur die gesamte Virusforschung 14:27–38
    [Google Scholar]
  14. McChesney M. B., Kehrl J. H., Valsamakis A., Fauci A. S., Oldstone M. B. A. 1987; Measles virus infection of B lymphocytes permits cellular activation but blocks progression through the cell cycle. Journal of Virology 61:3441–3447
    [Google Scholar]
  15. McChesney M. B., Oldstone M. B. A. 1989; Virus-induced immunosuppression: infections with measles and human immunodeficiency virus. Advances in Immunology 45:335–380
    [Google Scholar]
  16. McCullough K. C., Obi T. U., Sheshberadaran H. 1991; Identification of epitope(s) on the internal virion proteins of rinderpest virus which are absent from peste des petits ruminants virus. Veterinary Microbiology 26:313–321
    [Google Scholar]
  17. Maury W. 1994; Monocyte maturation controls expression of equine infectious anemia virus. Journal of Virology 68:6270–6279
    [Google Scholar]
  18. Norrby E., Chen S. N., Togashi T., Sheshberadaran H., Johnson K. P. 1982; Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells. Archives of Virology 71:1–11
    [Google Scholar]
  19. Outteridge P. M., Lee C. S. 1981; Cellular immunity in the mammary gland with particular reference to T, B lymphocytes and macrophages. In The Ruminant Immune System pp 513–534 Edited by Butler J. E. New York: Plenum Press;
    [Google Scholar]
  20. Pigoury L., Vacher B., Chabassol C., Poussot A. 1967; Note sur la culture du virus bovipestique lapinisé en cultures cellulaires de leucocytes de boeuf. Annales de ľ Institut Pasteur 113:613–634
    [Google Scholar]
  21. Plowright W. 1964; Studies on the pathogenesis of rinderpest in experimental cattle, II. Proliferation of the virus in different tissues following intranasal infection. Journal of Hygiene 62:257–281
    [Google Scholar]
  22. Plowright W., Ferris R. D. 1962; Studies with rinderpest virus in tissue culture. The use of attenuated culture virus as a vaccine for cattle. Research in Veterinary Science 3:172–182
    [Google Scholar]
  23. Reed L. J., Muench H. 1938; A simple method for estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  24. Rossiter P. B., Wardley R. C. 1985; The differential growth of virulent and avirulent strains of rinderpest virus in bovine lymphocytes and macrophages. Journal of General Virology 66:969–975
    [Google Scholar]
  25. Rossiter P. B., Wafula J. S., Gumm I. D., Stagg D. A., Morzaria S. P., Shaw M. K. 1988; Growth of rinderpest and bovine virus diarrhoea viruses in Theileria parva infected lymphoblastoid cell lines. Veterinary Record 122:491–492
    [Google Scholar]
  26. Rossiter P. B., Herniman K. A. J., Gumm I. D., Morrison W. I. 1993; The growth of cell culture-attenuated rinderpest virus in bovine lymphoblasts with B cell, CD4+ and CD8+ alpha/beta T cell and gamma/delta T cell phenotypes. Journal of General Virology 74:305–309
    [Google Scholar]
  27. Salonen R., Ilonen J., Salmi A. 1988; Measles virus infection of unstimulated blood mononuclear cells in vitro: antigen expression and virus production preferentially in monocytes. Clinical and Experimental Immunology 71:224–228
    [Google Scholar]
  28. Scott G. R. 1990; Rinderpest virus. In Viral Infections of Ruminants pp 341–354 Edited by Dinter Z., Morein B. Amsterdam: Elsevier Science Publishers;
    [Google Scholar]
  29. Sheshberadaran H., Norrby E., McCullough K., Carpenter W., Örvell C. 1986; The antigenic relationship between measles, canine distemper and rinderpest viruses studied with monoclonal antibodies. Journal of General Virology 67:1381–1392
    [Google Scholar]
  30. Sullivan J. L., Barry D. W., Lucas S. J., Albrecht P. 1975; Measles infection of human mononuclear cells. Journal of Experimental Medicine 142:773–784
    [Google Scholar]
  31. Taylor W. P., Plowright W. 1965; Studies on the pathogenesis of rinderpest in experimental cattle. III. Proliferation of an attenuated strain in various tissues following subcutaneous inoculation. Journal of Hygiene 63:263–275
    [Google Scholar]
  32. Tokuda G., Fukusho K., Morimoto T., Watanabe M. 1962; Studies on rinderpest virus in bovine leukocyte culture. National Institute of Animal Health Quarterly 2:189–200
    [Google Scholar]
  33. Trautwein G. 1992; Immune mechanisms in the pathogenesis of viral diseases: a review. Veterinary Microbiology 33:19–34
    [Google Scholar]
  34. Von Pirquet C. 1908; Verhalten der kutanen Tuberkulin-Reaktion wahrend der Masern. Deutsch Medizinische Wochenzeitschrift 34:1297–300
    [Google Scholar]
  35. Yamanouchi K., Chino F., Kobune F., Fukuda A., Yoshikawa Y. 1974a; Pathogenesis of rinderpest virus infection in rabbits. I. Clinical signs, immune response, histopathological changes and virus growth patterns. Infection and Immunity 9:199–205
    [Google Scholar]
  36. Yamanouchi K., Fukuda A., Kobune F., Yoshikawa Y., Chino F. 1974b; Pathogenesis of rinderpest virus infection in rabbits. II. Effect of rinderpest virus on the immune function of rabbits. Infection and Immunity 9:206–211
    [Google Scholar]
  37. Ziegler-Heitbrock H. W. L., Ulevitch R. J. 1993; CD14: cell surface receptor and differentiation marker. Immunology Today 14:121–125
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-76-11-2779
Loading
/content/journal/jgv/10.1099/0022-1317-76-11-2779
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error