1887

Abstract

A previously reported spurious serological crossreaction between alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV), which had been defined by the reaction in gel-immunodiffusion tests of a single IgM monoclonal antibody (MAb), MAb 8, was no longer detected in the presence of 0·1 -NaCl. The non-specific reactivity of this IgM was also confirmed in Western blotting assays. When skimmed milk was used as a blocking agent and as a diluent of antibodies, MAb 8 failed to recognize AMV and CMV coat proteins. Hence, it is concluded that the alleged cross-reaction between AMV and CMV is due to non-specific binding of MAb 8 and that there is no evidence for a serological relationship between these two viruses.

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/content/journal/jgv/10.1099/0022-1317-75-2-463
1994-02-01
2021-10-19
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References

  1. Altschuh D., Van Regenmortel M. H. V. 1983; Refutation by ELISA of the alleged antigenic relationship between an isometric and a rod-shaped plant virus. International Journal of Microbiology 1:13–17
    [Google Scholar]
  2. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  3. Crowle A. J. 1973 Immunodiffusion, 2nd edn. New York: Academic Press;
    [Google Scholar]
  4. Dietzgen R. G., Zaitlin M. 1991; Alleged common antigenic determinants of tobacco mosaic virus coat protein and the host protein ribulose-l,5-bisphosphate carboxylase is an artifact of indirect ELISA and Western blotting. Virology 184:397–398
    [Google Scholar]
  5. Hajimorad M. R., Francki R. I. B. 1989; Preparation of soluble, biologically active alfalfa mosaic virus coat protein and its CaCl2-induced degradation. Journal of Virological Methods 25:49–61
    [Google Scholar]
  6. Hajimorad M. R., Francki R. I. B. 1991; Effect of glutaraldehyde-fixation on the immunogenicity, particle stability and antigenic reactivity of alfalfa mosaic virus, and the specificity of elicited antibodies. Journal of Virological Methods 33:13–25
    [Google Scholar]
  7. Hajimorad M. R., Dietzgen R. G., Francki R. I. B. 1990; Differentiation and antigenic characterization of closely related alfalfa mosaic virus strains with monoclonal antibodies. Journal of General Virology 71:2809–2816
    [Google Scholar]
  8. Johnson D. A., Gautsch J. W., Sportsman J. R., Elder J. H. 1984; Improved technique utilizing non fat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose. Gene Analysis Techniques 1:3–8
    [Google Scholar]
  9. Matthews J. S., Brooker J. D. 1991; The isolation and characterization of polyclonal antibodies to anastomosis group 8 of Rhizoctonia solani . Plant Pathology 40:67–77
    [Google Scholar]
  10. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  11. Van Vloten-Doting L., Francki R. I. B., Fulton R. W., Kaper J.M., Lane L. C. 1981; Tricornaviridae - a proposed family of plant viruses with tripartite, single-stranded RNA genomes. Intervirology 15:198–203
    [Google Scholar]
  12. Wahyuni W. S., Dietzgen R. G., Hanada K., Francki R. I. B. 1992; Serological and biological variation between and within subgroup I and II strains of cucumber mosaic virus. Plant Pathology 41:282–297
    [Google Scholar]
  13. Zimmermann D., Van Regenmortel M. H. V. 1989; Spurious cross-reactions between plant viruses and monoclonal antibodies can be overcome by saturating ELISA plates with milk proteins. Archives of Virology 106:15–22
    [Google Scholar]
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