%0 Journal Article %A Hajimorad, M. R. %A Dietzgen, R. G. %A Wahyuni, W. S. %A Francki, R. I. B. %T Alleged reaction in gel-immunodiffusion of an IgM monoclonal antibody with alfalfa mosaic virus and cucumber mosaic virus is an artefact %D 1994 %J Journal of General Virology, %V 75 %N 2 %P 463-465 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-75-2-463 %I Microbiology Society, %X A previously reported spurious serological crossreaction between alfalfa mosaic virus (AMV) and cucumber mosaic virus (CMV), which had been defined by the reaction in gel-immunodiffusion tests of a single IgM monoclonal antibody (MAb), MAb 8, was no longer detected in the presence of 0ยท1 m-NaCl. The non-specific reactivity of this IgM was also confirmed in Western blotting assays. When skimmed milk was used as a blocking agent and as a diluent of antibodies, MAb 8 failed to recognize AMV and CMV coat proteins. Hence, it is concluded that the alleged cross-reaction between AMV and CMV is due to non-specific binding of MAb 8 and that there is no evidence for a serological relationship between these two viruses. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-75-2-463