1887

Abstract

The complete genome of a hepatitis B virus (HBV) from Brazil that expressed the subtype of HBV surface antigen (HBsAg) was cloned and sequenced. The genome, termed w4B, consists of 3215 bp. The overall genetic organization of typical hepadnaviruses with four open reading frames including the preC region was found to be conserved. When comparing the w4B sequence with 19 complete HBV genomes it was, however, found to be more divergent (15%) than any other HBV sequence thus far reported. Until now, no more than 11% divergence has been reported. Distinct from the five known HBV genotypes A to E, w4B made up a new, sixth genotype. The importance of the conserved third start codon in the HBV X gene became apparent in isolate w4B. By mutation, this ATG was out of frame, and by what appears to have been a linked mutation, a new start site two codons downstream was re-established. The significance of several other mutations is discussed.

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1993-08-01
2021-10-16
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References

  1. Ashton-Rickardt PG, Murray K. Mutations that change the immunological subtype of hepatitis b virus surface antigen and distinguish between antigenic and immunogenic determination.. Journal of Medical Virology 29:204–214 1989
    [Google Scholar]
  2. Bancroft WH, Mundon FK, Russell K. Detection of additional antigenic determinants of hepatitis B antigen. Journal of Immunology 109:842–848 1972
    [Google Scholar]
  3. Bichko V, Pushko P, Dreilina D, Pumpen P, Gren E. Subtype ayw variant of hepatitis B virus. FEBS Letters 185:208–212 1985
    [Google Scholar]
  4. Blum HE, Galun E, Liang TJ, von Weizsäcker F, Wands JR. Naturally occurring missense mutation in the polymerase gene terminating hepatitis B virus replication. Journal of Virology 65:1836–1842 1991
    [Google Scholar]
  5. Blum HE, Zhang ZS, Galun E, von Weizsäcker F, Garner B, Liang TJ, Wands JR. Hepatitis B virus X protein is not central to the viral life cycle in vitro. Journal of Virology 66:1223–1227 1992
    [Google Scholar]
  6. Carman WF, Thomas HC. Genetically defined variants of hepatitis B virus. Reviews in Medical Virology 1:29–39 1991
    [Google Scholar]
  7. Chen HS, Kaneko S, Girones R, Anderson RW, Hornbuckle WE, Tennant BC, Cote PJ, Gerin JL, Purcell RH, Miller RH. The woodchuck hepatitis virus X gene is important for establishment of virus infection in woodchucks. Journal of Virology 67:1218–1226 1993
    [Google Scholar]
  8. Couroucé AM, Soulier JP. The a(w) Subdeterminants. Current Studies in Hematology and Blood Transfusion 42:31–41 1976
    [Google Scholar]
  9. Couroucé-Pauty AM, Plançon A, Soulier JP. Distribution of HBsAg Subtypes in the World. Vox Sanguinis 44:197–211 1983
    [Google Scholar]
  10. Estacio RC, Chavez CC, Okamoto H, Lingao AL, Reyes MT, Domingo E, Mayumi M. Nucleotide sequence of a hepatitis B virus genome of subtype adw isolated from a Philippine: Comparison with the reported three genomes of the same subtype. Journal of Gastroenterology and Hepatology 3:215–222 1988
    [Google Scholar]
  11. Feng DF, Doolittle RF. Progressive sequence alignment as a prerequisitetto correct phylogenetic trees. Journal of Molecular Evolution 25:351–360 1987
    [Google Scholar]
  12. Fujiyama A, Miyanohara A, Nozaki C, Yoneyama T, Ohtomo N, Matsubara K. Cloning and structural analyses of hepatitis B virus DNAs, subtypeadr. Nucleic Acids Research 11:4601–4610 1983
    [Google Scholar]
  13. Galibert F, Mandart E, Fitoussi F, Tiollais P, Charnay P. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli. Nature 281:646–650 1979
    [Google Scholar]
  14. Gan R, Chu M, Shen L, Qian S, Li Z. The complete nucleotide sequence of the cloned DNA of hepatitis B virus subtype adr in pADR-1. Scientia sinica 30:507–521 1987
    [Google Scholar]
  15. Gaspar AMC, Yoshida CFT. Geographic distribution of HBsAg subtypes in Brazil. Memórias do Instituto Oswaldo Cruz 82:253–258 1987
    [Google Scholar]
  16. Hatton T, Zhou S, Standring DN. RNA- and DNA-binding activities in hepatitis B virus capsid protein: a model for their roles in viral replication. Journal of Virology 66:5232–5241 1992
    [Google Scholar]
  17. Heermann KH, Goldmann U, Schwartz W, Seyffarth T, Baumgarten H, Gerlich WH. Large surface proteins of hepatitis B virus containing the preS sequence.. Journal of Virology 52:396–402 1984
    [Google Scholar]
  18. Howard CR. Hepadnaviridae. Classification and Nomenclature of Viruses. Fifth report of the International Committee on Taxonomy of Viruses pp 111–116 1991; Edited by R. I. B. Francki, C. M. Fauquet, D. L. Knudson & F. Brown.. Archives of Virology supplementum 2 New York: Springer-Verlag;
    [Google Scholar]
  19. Junker-Niepmann M, Bartenschlager R, Schaller H. A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA.. The EMBO Journal 9:3389–3396 1990
    [Google Scholar]
  20. Kim KT, Hyun SW, Kim YS, Rho HM. Complete nucleotide sequence of hepatitis B virus (subtype adr).. Korean Biochemical Journal 21:319–331 1988
    [Google Scholar]
  21. Kobayashi M, Katsuro K. Complete nucleotide sequence of hepatitis B virus DNA of subtype adr and its conserved gene organization. Gene 30:227–232 1984
    [Google Scholar]
  22. Kwee L, Lucito R, Aufiero B, Schneider RJ. Alternate translation initiation on hepatitis B virus X mRNA produces multiple polypeptides that differentially transactivate class II and III promoters. Journal of Virology 66:4382–4389 1992
    [Google Scholar]
  23. Lundberg KS, Shoemaker DD, Adams MWW, Short JM, Sorge JA, Mathur EJ. High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene 108:1–6 1991
    [Google Scholar]
  24. Le Bouvler GL. Seroanalysis by immunediffusion: the subtypes of type B hepatitis virus.. Hepatitis andBlood Transfusion97–110 1971; Edited by G. N. Vyas, A. Perkins & R. Schmid.. New York: Grune & Stratton.;
    [Google Scholar]
  25. Nassal M. The arginine-rich domain of the hepatitis B virus core protein is required for pregenome encapsidation and productive viral positive-strand DNA synthesis but not for virus assembly. Journal of Virology 66:4107–4116 1992
    [Google Scholar]
  26. Norder H, Couroucé AM, Magnius LO. Molecular basis of hepatitis B virus serotype variations within the four major subtypes.. Journal of General Virology 73:3141–3145 1992a
    [Google Scholar]
  27. Norder H, Hammas B, Lofdahl S, Couroucé AM, Magnius LO. Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains. Journal of General Virology 73:1201–1208 1992b
    [Google Scholar]
  28. Norder H, Couroucé AM, Magnius LO. Complete nucleotide sequences of six hepatitis B viral genomes encoding the surface antigen subtypes ayw4, adw4q-, and adrq- and their phylogenetic classification. Research in Chronic Viral Hepatitis189–199 1993
    [Google Scholar]
  29. Okamoto H, Imai M, Shimozaki M, Hoshi Y, Iizuka H, Gotanda T, Tsuda F, Miyakawa Y, Mayumi M. Nucleotide Sequence of a Cloned Hepatitis B Virus Genome, Subtype ayr: Comparison with Genomes of the Other Three Subtypes. Journal of General Virology 67:2305–2314 1986
    [Google Scholar]
  30. Okamoto H, Imai M, Tsuda F, Tanaka T, Miyakawa Y, Mayumi M. Point mutation in the S gene of hepatitis B virus for a d/y or w/r subtypic change in two blood donors carrying a surface antigen of compound subtype adyr or adwr. Journal of Virology 61:3030–3034 1987
    [Google Scholar]
  31. Okamoto H, Tsuda F, Sakugawa H, Sastrosoewignjo RI, Imai M, Miyakawa Y, Mayumi M. Typing Hepatitis B Virus by Homology in Nucleotide Sequence: Comparison of Surface Antigen Subtypes. Journal of General Virology 69:2575–2583 1988
    [Google Scholar]
  32. Ono Y, Onda H, Sasada R, Igarashi K, Sugino Y, Nishioka K. Tbe complete nudeotide sequences of the cloned hepatitis B vims DNA; subtype adr and adw. Nucleic Acids Research 11:1747–1757 1983
    [Google Scholar]
  33. Peterson DL, Paul DA, Lam L, Tribby II, Achord DT. Antigenic structure of hepatitis B surface antigen: identification of theʹ dʹ subtype determinant by chemical modification and use of monoclonal antibodies.. Journal of Immunology 132:920–927 1984
    [Google Scholar]
  34. Radziwill G, Tucker W, Schaller H. Mutational analysis of the hepatitis B virus P gene product: domain structure and RNase H activity. Journal of Virology 64:613–620 1990
    [Google Scholar]
  35. Rho HM, Kim K, Hyun SW, Kim YS. The nucleotide sequence and reading frames of a mutant hepatitis B virus adr.. Nucleic Acids Research 17: 1989
    [Google Scholar]
  36. Roychoudhury S, Faruqi AF, Shih C. Pregenomic RNA encapsidation analysis of eleven missense and nonsense polymerase mutants of human hepatitis B virus. Journal of Virology 65:3617–3624 1991
    [Google Scholar]
  37. Salfeld J, Pfaff E, Noah M, Schaller H. Antigenic determinants and functional domains in core antigen and e antigen from hepatitis B virus. Journal of Virology 63:798–808 1989
    [Google Scholar]
  38. Sastrosoewignjo RL, Omi S, Okamoto H, Mayumi M, Rustam M, Stsjudi T. The complete nucleotide sequence of HBV DNA clone of subtype adw (pMND122) from Menado in Sulawesi IslandIndonesia.. International Center for Medical Research Annals 7:51–60 1987
    [Google Scholar]
  39. Schüler A. Nukleotidsequenzanalyse eines Hepatitis B Virusgenoms aus dem Isolat 991 Subtyp adw 1990; M. D. thesis, Gǒttingen. EMBI. accession no.: X51970..
    [Google Scholar]
  40. Spandau DF, Lee CH. trans-activation of viral enhancers by the hepatitis B virus X protein. Journal of Virology 62:427–434 1988
    [Google Scholar]
  41. Stirk HJ, Thornton JM, Howard CR. Atopological Model for Hepatitis B Surface Antigen. Intervirology 33:148–158 1992
    [Google Scholar]
  42. Tong S, Li J, Vitvitski L, Trépo C. Active hepatitis B virus replication in the presence of anti-HBe is associated with viral variants containing an inactive pre-C region. Virology 176:596–603 1990
    [Google Scholar]
  43. Twu JS, Schloemer RH. Transcriptional trans-activating function of hepatitis B virus. Journal of Virology 61:3448–3453 1987
    [Google Scholar]
  44. Valenzuela P, Quiroga M, Zaldivar J, Gray P, Rutter WJ. 1980; The nucleotide sequence of the hepatitis B viral genome and the identification of the major viral genes. Animal Virus Genetics pp 57–70 Edited by B. N. Fields, R. Jaenisch & & C. F. Fox.. New York: Academic Press.;
    [Google Scholar]
  45. Vaudin M, Wolstenholme AJ, Tsiquaye KN, Zuckerman AJ, Harrison TJ. The Complete Nucleotide Sequence of the Genome of a Hepatitis B Virus Isolated from a Naturally Infected Chimpanzee. Journal of General Virology 69:1383–1389 1988
    [Google Scholar]
  46. Will H, Reiser W, Weimer T, Pfaff E, Büscher M, Sprengel R, Cattaneo R, Schaller H. Replication strategy of human hepatitis B virus. Journal of Virology 61:904–911 1987
    [Google Scholar]
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