1887

Abstract

The gene encoding the major nucleocapsid, N, protein of measles virus has been inserted into a baculovirus vector under the control of the polyhedrin promoter. Insect cells infected with this recombinant baculovirus synthesize high levels of measles N protein, up to 40% of total soluble cell protein. The recombinant protein is recognized by sera from convalescent patients, vaccinees and patients with subacute sclerosing panencephalitis and thus could form the basis of a simple diagnostic assay. Nucleocapsid-like structures, similar to those found in mammalian cells infected with measles virus, can be observed in both the nucleus and cytoplasm of the infected insect cells. These have many structural features in common with nucleocapsids found in measles virus-infected cells, but are longer (up to 2 µm) and have a lower buoyant density. Measles N protein thus appears to be capable of assembling into nucleocapsidlike structures in the absence of measles virion RNA or other viral proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-74-7-1439
1993-07-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/jgv/74/7/JV0740071439.html?itemId=/content/journal/jgv/10.1099/0022-1317-74-7-1439&mimeType=html&fmt=ahah

References

  1. Baker SC, Moyer SA. 1988; Encapsidation of Sendai virus genome RNAs by purified NP protein during in vitro replication.. Journal of Virology 62:834–838
    [Google Scholar]
  2. Bankamp B, Brinckmann UG, Reich A, Niewiesk S, ter Meulen V, Liebert UG. 1991; Measles virus nucleocapsid protein protects rats from encephalitis. Journal of Virology 65:1695–1700
    [Google Scholar]
  3. Barrett T, Subbarao SM, Belsham GJ, Mahy BWJ. 1991; The Molecular Biology of the Morbilliviruses. Edited by Kingsbury DW. In The Paramyxoviruses New York & London: Plenum Press; pp 83–102
    [Google Scholar]
  4. Blumberg BM, Giorgi C, Kolakofsky D. 1983; N protein of vesicular stomatitis virus selectively encapsidates leader RNA in vitro.. Cell 32:559–657
    [Google Scholar]
  5. Cattaneo R, Schmid A, Spielhofer P, Kaelin K, Baczko K, Ter Meulen V., Pardowitz J, Flanagan S, Rima B, Udem SA, Billeter MA. 1989; Mutated and hypermutated genes of persistent measles viruses which cause lethal human brain diseases.. Virology 173:415–425
    [Google Scholar]
  6. Dingwall C, Laskey RA. 1991; Nuclear targeting sequences -a consensus?. Trends in Biochemical Sciences 16:478–481
    [Google Scholar]
  7. Dubois-Dalcq M, Coblentz JM, Pleet AB. 1974; Subacute sclerosing panencephalitis. Unusual nuclear inclusions and lengthy clinical course.. Archives of Neurology 31:355–364
    [Google Scholar]
  8. Gershon A, Krugman S. 1979; Measles. Diagnostic Procedures for Viral and Rickettsial Infections, 5th edn.Edited by Schmidt NJ, Emmons RW. Washington, D.C.: American Public Health Association.; p In 665
    [Google Scholar]
  9. Henderson RH, Keja JK, Haydin G. 1988; Immunising the children of the world: progress and prospects.. Bulletin of the World Health Organization 66:535–545
    [Google Scholar]
  10. Maniatis T, Fritsch EF, Sambrook J. 1989 Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  11. Ray R, Roux L, Compans RW. 1991; Intracellular targeting and assembly of paramyxovirus proteins. Edited by Kingsbury DW. The Paramyxoviruses New York: Plenum Press; pp In 247–180
    [Google Scholar]
  12. Reynolds ES. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.. Journal of Cell Biology 17:208–212
    [Google Scholar]
  13. Rima BK. 1983; The proteins of morbilliviruses.. Journal of General Virology 64:1205–1219
    [Google Scholar]
  14. Robbins SJ. 1983; Progressive invasion of cell nuclei by measles virus in persistently infected human cells.. Journal of General Virology 64:2335–2338
    [Google Scholar]
  15. Rozenblatt S, Eizenberg O, BenLevy R, La vie V, Bellini WJ. 1985; Sequence homology within the morbilliviruses.. Journal of Virology 53:684–690
    [Google Scholar]
  16. Schmid A, Cattaneo R, Billeter MA. 1987; A procedure for selective full length cDNA cloning of specific RNA species.. Nucleic Acids Research 15:3987–3996
    [Google Scholar]
  17. Spehner D, Kirn A, Drillien R. 1991; Assembly of nucleocapsid-like structures in animal cells infected with a vaccinia virus recombinant encoding the measles virus nucleoprotein.. Journal of Virology 65:6296–6300
    [Google Scholar]
  18. Stephenson JR, Ter Meulen, V. 1979; Antigenic relationships between measles and canine distemper viruses: comparison of the immune response in animals and humans to individual virus-specific polypeptides.. Proceedings of the National Academy of Sciences, U.S.A. 76:6601–6605
    [Google Scholar]
  19. Stephenson JR, Hay AJ, Skehel JJ. 1977; Characterization of virus-specific messenger RNAs from avian fibroblasts infected with fowl plague virus.. Journal of General Virology 36:237–248
    [Google Scholar]
  20. Summers MD, Smith GE. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures.. Texas Agricultural Experimental Station Bulletin no 1555
    [Google Scholar]
  21. ter Meulen V, Stephenson JR, Kreth HW. 1983; Subacute sclerosing panencephalitis.. Comprehensive Virology 18:105–160
    [Google Scholar]
  22. Thorne HV, Dermott E, Kreth HW. 1976; Circular and elongated linear forms of measles virus nucleocapsid.. Nature, London 264:473–474
    [Google Scholar]
  23. Vialard J, Lalumière M, Vernet T, Briedis D, Alkhatib G, Henning D, Levin D, Richardson C. 1990; Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector containing the beta-galactosidase gene. Journal of Virology 64:37–50
    [Google Scholar]
  24. Warnes A, Walkland A, Stephenson JR. 1986; Development of an enzyme-linked immunosorbent assay for staphylococcal protein A produced in Escherichia coli by pUC8 based plasmids containing the Staphylococcus aureus Cowan I protein A gene. Journal of Immunological Methods 93:63–70
    [Google Scholar]
  25. Waters DJ, Bussell RH. 1974; solation and comparative study of the nucleocapsids of measles and canine distemper viruses from infected cells.. Virology 61:64–79
    [Google Scholar]
  26. Waters DJ, Hersch RT, Bussell RH. 1962; Isolation and characterisation of measles virus nucleocapsids from infected cells. Virology 64:1205–1219
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-74-7-1439
Loading
/content/journal/jgv/10.1099/0022-1317-74-7-1439
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error