1887

Abstract

A lentivirus has been isolated from a Finnish ewe with ovine progressive pneumonia in a closed upstate New York flock. We demonstrated that the virus, designated ovine lentivirus strain CU1 (OLV-CU1), is biologically, biochemically and molecularly related to, but distinct from, previously described sheep and goat lentiviruses. Nine of 32 ewes (from the affected flock) with precipitating antibodies for ovine lentivirus also produced antibodies that were able to neutralize the infectivity of OLV-CU1. The virus replicated in cultured sheep fibroblasts and caused the formation of large multi-nucleated cells. OLV-CU1-specific RNA transcripts found in infected cells and virion antigenic proteins were similar to those of other small ruminant lentiviruses. However, the virus was distinguished from other isolates at the DNA level by nucleic acid hybridization, restriction endonuclease mapping and partial sequencing of the virus genome.

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1993-02-01
2022-11-27
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References

  1. Balfe P., Simmonds P., Ludlam C. E., Bishop J. O., Leigh-Brown A. 1990; Concurrent evolution of human immunodeficiency virus type 1 in patients infected from the same source: rate of sequence change and low frequency of inactivating mutations. Journal of Virology 64:6221–6233
    [Google Scholar]
  2. Berkhout B., Gatignol A., Rabson A. B., Jeang K. 1990; TAR-independent activation of the HIV-1 LTR: evidence that Tat requires specific regions of the promoter. Cell 62:757–767
    [Google Scholar]
  3. Brahic M., Haase A. T. 1978; Detection of viral sequences oflow reiteration frequency by in situ hybridization. Proceedings of the National Academy of Sciences, U.S.A 756125–6129
    [Google Scholar]
  4. Braun M. J., Clements J. E., Gonda M. A. 1987; The visna virus genome: evidence for a hypervariable site in the env gene and sequence homology among lentivirus envelope proteins. Journal of Virology 61:4046–4054
    [Google Scholar]
  5. Chinsky J., Soeiro R. 1981; Fv-1 host restriction of friend leukemia virus: analysis of unintegrated proviral DNA. Journal of Virology 40:45–55
    [Google Scholar]
  6. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  7. Colvin R. A., Garcia-Blanco M. A. 1992; Unusual structure of the human immunodeficiency virus type 1 trans-activation response element. Journal of Virology 66:930–935
    [Google Scholar]
  8. Cullen B. R. 1991; Regulation of HIV-1 gene expression. FASEB Journal 5:2361–2368
    [Google Scholar]
  9. Cutlip R. C., Laird G. A. 1976; Isolation and characterization of a virus associated with progressive pneumonia (maedi) of sheep. American Journal of Veterinary Research 37:1377–1382
    [Google Scholar]
  10. Cutlip R. C., Jackson T. A., Lehmkuhl H. D. 1978; Diagnostic features of ovine progressive pneumonia. Journal of the American Veterinary Medical Association 173:1578–1579
    [Google Scholar]
  11. Cutlip R. C., Lehmkuhl H. D., Brogden K. A., Sacks J. M. 1986; Breed susceptibility to ovine progressive pneumonia (maedi/ visna) virus. Veterinary Microbiology 12:283–288
    [Google Scholar]
  12. Davis J. L., Clements J. E. 1989; Characterization of a cDNA clone encoding the visna virus transactivation protein. Proceedings of the National Academy of Sciences, U.S.A 86414–418
    [Google Scholar]
  13. Davis J. L., Molineaux S., Clements J. E. 1987; Visna virus exhibits a complex transcriptional pattern: one aspect of gene expression shared with the acquired immunodeficiency syndrome retrovirus. Journal of Virology 61:1325–1331
    [Google Scholar]
  14. De Boer G. F. 1975; Zwoegerziekte virus, the causative agent for progressive interstitial pneumonia (maedi) and meningo-encephalitis (visna) in sheep. Research in Veterinary Science 18:15–25
    [Google Scholar]
  15. Ellis J. 1983; Ovine progressive pneumonia. Veterinary Medicine and Small Animal Clinician 78:1247–1256
    [Google Scholar]
  16. Garcia J. A., Harrich D., Pearson L., Mitsuyasu R., Gaynor R. B. 1988; Functional domains required for tat-induced transcriptional activation of the HIV-1 long terminal repeat. EMBO Journal 7:3143–3147
    [Google Scholar]
  17. Gdovin S. L., Clements J. E. 1992; Molecular mechanisms of visna virus Tat: identification of the targets for transcriptional activation and evidence for a post-transcriptional effect. Virology 188:438–450
    [Google Scholar]
  18. Gendelman H. E., Narayan O., Kennedy-Stoskopf S., Kennedy P. G., Ghotbi Z., Clements J. E., Stanley J., Pezeshkpour G. 1986; Tropism of sheep lentiviruses for monocytes: susceptibility to infection and virus gene expression increase during maturation of monocytes to macrophages. Journal of Virology 58:67–74
    [Google Scholar]
  19. Gogolewski R. P., Adams D. S., McGuire T. C., Banks K. L., Cheevers W. P. 1985; Antigenic cross-reactivity between caprine arthritis-encephalitis, visna and progressive pneumonia viruses involves all virion-associated proteins and glycoproteins. Journal of General Virology 66:1233–1240
    [Google Scholar]
  20. Haase A. T., Stowring L., Harris J. D., Traynor B., Ventura P., Peluso R., Brahic M. 1982; Visna DNA synthesis and the tempo of infection in vitro. Virology 119:399–410
    [Google Scholar]
  21. Harter D. H., Choppin P. W. 1967; Cell fusing activity of visna virus particles. Virology 31:279–288
    [Google Scholar]
  22. Heine C. W., Kelly D. C., Avery R. J. 1980; The detection of intracellular retrovirus-like entities in Drosophila melanogaster cell cultures. Journal of General Virology 49:385–395
    [Google Scholar]
  23. Hess J. L., Pyper J. M., Clements J. E. 1986; Nucleotide sequence and transcriptional activity of the caprine arthritis-encephalitis virus long terminal repeat. Journal of Virology 60:385–393
    [Google Scholar]
  24. Higgins D. G., Sharp P. M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5:151–153
    [Google Scholar]
  25. Higgins D. G., Bleasby A. J., Fuchs R. 1991; Clustal V: improved software for multiple sequence alignment. CABIOS 8:189–191
    [Google Scholar]
  26. Hirsch V. M., Olmsted R. A., Murphey-Corb M., Purcell R. H., Johnson P. R. 1989; An African primate lentivirus (SIVsm) closely related to HIV-2. Mature, London 339:389–392
    [Google Scholar]
  27. Houwers D. J. 1989; Importance of ewe/lamb relationship and breed in the epidemiology of maedi-visna virus infections. Research in Veterinary Science 46:5–8
    [Google Scholar]
  28. Jackson M. K., Knowles D. P., Stem T. A., Harwood W. G., Robinson M. M., Cheevers W. P. 1991; Genetic structure of the pol-env region of the caprine arthritis-encephalitis lentivirus genome. Virology 180:389–394
    [Google Scholar]
  29. Kato H., Sumimoto H., Pognonec P., Chen C. H., Rosen C. A., Roeder R. G. 1992; HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors. Genes and Development 6:655–666
    [Google Scholar]
  30. Kennedy R. C., Eklund C. M., Lopez C., Had Low W. J. 1968; Isolation of a virus from the lungs of Montana sheep affected with progressive pneumonia. Virology 35:382–384
    [Google Scholar]
  31. Keohavong P., Thilly W. G. 1989; Fidelity of DNA polymerases in DNA amplification. Proceedings of the National Academy of Sciences, U.S.A 869253–9257
    [Google Scholar]
  32. Klein J. R., Martin J., Griffing S., Nathanson N., Gorham J., Shen D. T., Petursson G., Georgsson G., Palsson P. A., Lutley R. 1985; Precipitating antibodies in experimental visna and natural progressive pneumonia of sheep. Research in Veterinary Science 38:129–133
    [Google Scholar]
  33. Lairmore M. D., Akita G. Y., Russell H. I., DeMartini J. C. 1987; Replication and cytopathic effects of ovine lentivirus strains in alveolar macrophages correlate with in vivo pathogenicity. Journal of Virology 61:4038–4042
    [Google Scholar]
  34. Lairmore M. D., Poulson J. M., Adducci T. A., DeMartini J. C. 1988; Lentivirus-induced lymphoproliferative disease —comparative pathogenicity of phenotypically distinct ovine lentivirus strains. American Journal of Pathology 130:80–89
    [Google Scholar]
  35. Lehmkuhl H. D., Cutlip R. C., Bolin S. R., Brogden K. A. 1985; Seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. American Journal of Veterinary Research 46:2601–2604
    [Google Scholar]
  36. Marsh H. 1923; Progressive pneumonia in sheep. Journal of the American Veterinary Medicine Association 62:458–473
    [Google Scholar]
  37. McGuire T. C., Brassfield A. L., Davis W. C., Cheevers W. P. 1987; Antigenic and structural variation of the p28 core polypeptide of goat and sheep retroviruses. Journal of General Virology 68:2259–2263
    [Google Scholar]
  38. Narayan O. 1983; Role of macrophages in the immunopathogenesis of visna-maedi of sheep. Progress in Brain Research 59:233–235
    [Google Scholar]
  39. Narayan O., Clements J. E. 1989; Biology and pathogenesis of lentiviruses. Journal of General Virology 70:1617–1639
    [Google Scholar]
  40. Narayan O., Cork L. C. 1985; Lentiviral diseases of sheep and goats: chronic pneumonia leukoencephalomyelitis and arthritis. Reviews of Infectious Diseases 7:89–98
    [Google Scholar]
  41. Narayan O., Kolinsky J. S., Clements J. E., Strandberg J. D., Griffin D. E., Cork L. C. 1982; Slow virus replication: the role of macrophages in the persistence and expression of visna viruses of sheep and goats. Journal of General Virology 59:345–356
    [Google Scholar]
  42. Narayan O., Kennedy-Stoskopf S., Sheffer D., Griffin D. E., Clements J. E. 1983; Activation of caprine arthritis-encephalitis virus expression during maturation of monocytes to macrophages. Infection and Immunity 41:67–73
    [Google Scholar]
  43. Narayan O., Clements J., Kennedy-Stoskopf S., Sheffer D., Royal W. 1987; Mechanisms of escape of visna lentiviruses from immunological control. Contributions in Microbiology and Immunology 8:60–76
    [Google Scholar]
  44. Petursson G., Hoff-Jorgensen R. 1990 Maedi-Visna and Related Diseases Boston: Kluwer Academic Publishers;
    [Google Scholar]
  45. Querat G., Barban V., Sauze N., Vigne R., Payne A., York D., De Villiers E. M., Verwoerd D. W. 1987; Characteristics of a novel lentivirus derived from South African sheep with pulmonary adenocarcinoma (jaagsiekte). Virology 158:158–167
    [Google Scholar]
  46. Querat G., Audoly G., Sonigo P., Vigne R. 1990; Nucleotide sequence analysis of SA-OMVV, a visna-related ovine lentivirus: phylogenetic history of lentiviruses. Virology 175:434–447
    [Google Scholar]
  47. Ruben S., Perkins A., Purcell R., Joung K., Sia R., Burghoff R., Haseltine W. A., Rosen C. A. 1989; Structural and functional characterization of human immunodeficiency virus tat protein. Journal of Virology 63:1–8
    [Google Scholar]
  48. Saltarelli M., Querat G., Konings D. A., Vigne R., Clements J. E. 1990; Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus. Virology 179:347–364
    [Google Scholar]
  49. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold spring Harbor Laboratory;
    [Google Scholar]
  50. Sargan D. R., Bennet I. D. 1989; A transcriptional map of visna virus: definition of the second intron structure suggests a rev-like gene product. Journal of General Virology 70:1995–2006
    [Google Scholar]
  51. Sargan D. R., Bennet I. D., Cousens C., Roy D. J., Blacklaws B.-A., Dalziel R. G., Watt N. J., McConnell I. 1991; Nucleotide sequence of EV1, a British isolate of maedi-visna virus. Journal of General Virology 72:1893–1903
    [Google Scholar]
  52. Sharp P. A., Marciniak R. A. 1989; HIV TAR: an RNA enhancer?. Cell 59:229–230
    [Google Scholar]
  53. Sheffield W. D., Nakayan O., Strandberg J. D., Adams R. J. 1980; Visna-maedi-like disease associated with an ovine retrovirus infection in a Corriedale sheep. Veterinary Pathology 17:544–552
    [Google Scholar]
  54. Sigurdsson B. 1954; Maedi, a slow progressive pneumonia of sheep: an epizootiological and a pathological study. British Veterinary Journal 110:225–770
    [Google Scholar]
  55. Smith C. 1992; Ovine lentivirus: a real or imagined threat?. Journal of the American Veterinary Association 200:139–143
    [Google Scholar]
  56. Sonigo P., Alizon M-, Staskus K-, Klatzmann D., Cole S., Danos O., Retzel E., Tiollais P., Haase A., Wain-Hobson S. 1985; Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus. Cell 42:369–382
    [Google Scholar]
  57. Staskus K. A., Retzel E. F., Lewis E. D., Silsby J. L., St Cyr S., Rank J. R., Wietgrefe S. W., Haase A. T., Cook R., Fast D., Geiser P. T., Harty J. T., Kong S. H., Lahti C. J., Neufeld T. P., Porter T. E-, Shoop E., Zachow K. R. 1991; Isolation of replication-competent molecular clones of visna virus. Virology 181:228–240
    [Google Scholar]
  58. Thompson D. R. 1989 Experimental studies with ovine progressive pneumonia virus infection of sheep Ph.D. thesis, Cornell University
    [Google Scholar]
  59. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 764350–4354
    [Google Scholar]
  60. Weber J. N., Weiss R. A. 1988; HIV infection: the cellular picture. Scientific American 259:100–109
    [Google Scholar]
  61. Weiss M. J., Sweet R. W., Gulati S. C., Harter D. H. 1976; Nucleic acid sequence relationship among ‘slow’ viruses of sheep. Virologyll395–401
    [Google Scholar]
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