1887

Abstract

The capacity for autonomous replication of the DNA A of African cassava mosaic virus (ACMV), a member of the bipartite geminiviruses infecting dicotyledonous plants, has been compared in host and non-host cells. A derivative of the ACMV DNA A was transfected into tobacco and maize protoplasts. Although ACMV is not able to infect maize, replication of the DNA A in maize protoplasts was observed to occur. The efficiency of replication was 10 to 20% of that seen in tobacco protoplasts. In both plant systems, replication was detected after the onset of cell division. ACMV replication in maize cells was compared to that of wheat dwarf virus and found to be 10 to 20% of that observed with the monocotyledon-specific virus. Insertion of 1165 bp of non-viral DNA into the ACMV DNA A prevented replication in maize but not in tobacco.

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1993-12-01
2022-10-07
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References

  1. Davies J. W., Stanley J. 1989; Geminivirus genes and vectors. Trends in Genetics 5:77–81
    [Google Scholar]
  2. Dixon L. K., Koenig I., Hohn T. 1983; Mutagenesis of cauliflower mosaic virus. Gene 25:189–199
    [Google Scholar]
  3. Etessami P., Callis R., Ellwood S., Stanley J. 1988; Delimitation of essential genes of CLV DNA 2. Nucleic Acids Research 16:4811–4829
    [Google Scholar]
  4. Etessami P., Saunders K., Watts J., Stanley J. 1991; Mutational analysis of complementary-sense of genes of African cassava mosaic virus DNA A. Journal of General Virology 72:1005–1012
    [Google Scholar]
  5. Fontes E. P. B., Luckow V. A., Hanley-Bowdoin L. 1992; A geminivirus replication protein is a sequence-specific DNA binding protein. Plant Cell 4:597–608
    [Google Scholar]
  6. Goodall G. J., Filipowicz W. 1991; Different effects of intron nucleotide compositions and secondary structure on pre-mRNA splicing in monocot and dicot plants. EMBO Journal 10:2635–2644
    [Google Scholar]
  7. Keith B., Chua N.-H. 1986; Monocot and dicot pre-mRNAs are processed with different efficiencies in transgenic tobacco. EMBO Journal 6:2419–2425
    [Google Scholar]
  8. Lazarowitz S. G. 1992; Geminiviruses: genome structure and gene function. Critical Reviews in Plant Sciences 11:327–349
    [Google Scholar]
  9. Lazarowitz S. G., Wu L. C., Rogers S. G., Elmer J. S. 1992; Sequence-specific interaction with the viral AL1 protein identifies a geminivirus DNA replication origin. Plant Cell 4:799–809
    [Google Scholar]
  10. Maliga P., Breznovitz A., Marton L. 1973; Streptomycin resistant plants from callus cultures of tobacco. Nature New Biology 244:29–30
    [Google Scholar]
  11. Maniatis T., Fritsch E. F., Sambrook J. 1982 >Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Matzeit V., Schaefer S., Kammann M., Schalk H.-J., Schell J., Gronenborn B. 1991; Wheat dwarf virus vectors replicate and express foreign genes in cells of monocotyledonous plants. Plant Cell 3:247–258
    [Google Scholar]
  13. Morris B. A. M., Richardson K. A., Haley A., Zhan X., Thomas J. E. 1992; The nucleotide sequence of the infectious cloned DNA component of tobacco yellow dwarf virus reveals features of geminiviruses infecting monocotyledonous plants. Virology 187:633–642
    [Google Scholar]
  14. Mullineaux P. M., Guerineau F., Accotto G.-P. 1990; Processing of complementary sense RNAs of digitaria streak virus in its host and in transgenic tobacco. Nucleic Acids Research 18:7259–7265
    [Google Scholar]
  15. Nagy J. I., Maliga P. 1976; Callus induction and plant regeneration from mesophyll protoplasts of Nicotiana sylvestris. Zeitschrift für Pflanzenphysiologie 78:453–155
    [Google Scholar]
  16. Negrutiu I., Shillito R. D., Potrykus I., Biasini G., Sala F. 1987; Hybrid genes in the analysis of transformation conditions. I. Setting up a simple method for direct gene transfer in plant protoplasts. Plant Molecular Biology 8:363–373
    [Google Scholar]
  17. Paszkowski J., Saul M. W. 1984; Direct gene transfer to plants. Methods in Enzymology 118:668–684
    [Google Scholar]
  18. Peterhans A., Datta S. K., Datta K., Goodall G. J., Potrykus I., Paszkowski J. 1990; Recognition efficiency of Dicotyledoneae- specific promoter and RNA processing signals in rice. Molecular and General Genetics 222:361–368
    [Google Scholar]
  19. Potrykus I., Shillito R. D. 1986; Protoplasts: isolation, culture, plant regeneration. Methods in Enzymology 118:549–578
    [Google Scholar]
  20. Schalk H.-J., Matzeit M., Schiller B., Schell J., Gronenborn B. 1989; WDV, a geminivirus of graminaceous plants needs splicing for replication. EMBO Journal 8:259–364
    [Google Scholar]
  21. Stanley J., Gay M. 1983; Nucleotide sequence of cassava latent virus DNA. Nature, London 301:260–262
    [Google Scholar]
  22. Townsend R., Watts J., Stanley J. 1986; Synthesis of viral DNA forms in Nicotianaplumbaginifolia protoplasts inoculated with cassava latent virus (CLV), evidence for the independent replication of one component of the CLV genome. Nucleic Acids Research 14:1253–1265
    [Google Scholar]
  23. Ward A., Etessami P., Stanley J. 1988; Expression of bacterial genes in plants mediated by infectious geminivirus DNA. EMBO Journal 7:1583–1587
    [Google Scholar]
  24. Woolston C. J., Reynolds H. V., Stacey N. J., Mullineaux P. M. 1989; Replication of WDV DNA in protoplasts and analysis of coat protein mutants in protoplasts and plants. Nucleic Acids Research 17:6029–6041
    [Google Scholar]
  25. Zhang S.-B., Guo Z. S., Qian Y. Q., Cai Q. G., Zhou Y. L. 1990; Factors influencing isolation, division and plant regeneration in maize (Zea mays L.) protoplast culture. Chinese Journal of Botany 2:18–25
    [Google Scholar]
  26. Zhang S.-B., Spangenberg G., Datta S., Potrykus I. 1991; Stable transformation of maize protoplasts. In Abstracts of 8th International Protoplast Symposium no. 174, Physiologia Plantarum 82:A31
    [Google Scholar]
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