1887

Abstract

Seven putative origins of DNA replication () were identified and located on the genome of multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV), when an improved infection-dependent replication assay was used. A threefold higher yield of amplified plasmid was achieved when an m.o.i. of 1 was used (instead of 25), and another twofold increase was obtained when the interval between transfection and infection was extended from 5 to 24 h. Six of the putative s were located in regions with homologous sequences. This suggests that all in AcMNPV are bifunctional, i.e. have both and enhancer activity for transcription. In addition to the six , the dIII-K fragment of AcMNPV was also identified to carry a putative , although this fragment does not contain an region. However, the individual role of these seven s during viral DNA replication, and whether they are all active simultaneously , is still unclear. The replication of an -containing plasmid starts at the same time (6 h post-infection) and proceeds at the same rate as viral DNA replication. A circular topology of -containing plasmids was a prerequisite for replication. Linear DNA, with an , did not replicate. Therefore, we suggest a theta structure or a rolling-circle as a model for baculovirus DNA replication.

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1993-12-01
2024-12-03
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References

  1. Arif B. M., Doerfler W. 1984; Identification and localization of reiterated sequences in the Choristoneura fumiferana MNPV genome. EMBO Journal 3:525–529
    [Google Scholar]
  2. Bergsma D. J., Olive D. M., Hartzell S. W., Subramanian K. N. 1983; Territorial limits and functional anatomy of the simian virus 40 replication origin. Proceedings of the National Academy of Sciences, U.S.A 79:381–385
    [Google Scholar]
  3. Carson D. D., Summers M. D., Guarino L. A. 1991; Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements. Journal of Virology 65:945–951
    [Google Scholar]
  4. Cochran M. A., Faulkner P. 1983; Location of homologous DNA sequences interspersed at five regions in the baculovirus AcMNPV genome. Journal of Virology 45:961–970
    [Google Scholar]
  5. de Villiers J., Schaffner W., Tyndall C., Lupton S., Kamen R. 1984; Polyoma virus DNA replication requires an enhancer. Nature, London 312:242–246
    [Google Scholar]
  6. Fields B. N., Knipe D. M. (editors) 1990Virology 2nd edn New York: Raven Press;
  7. Friesen P. D., Miller L. K. 1987; Divergent transcription of early 35- and 94-kilodalton protein genes encoded by the Hin dIII-K genome fragment of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 61:2264–2272
    [Google Scholar]
  8. Groebe D. R., Chung A. E., Ho C. 1990; Cationic lipid-mediated cotransfection of insect cells. Nucleic Acids Research 18:4033
    [Google Scholar]
  9. Guarino L. A., Summers M. D. 1986; Interspersed homologous DNA of Autographa californica nuclear polyhedrosis virus enhances delayed-early gene expression. Journal of Virology 60:215–223
    [Google Scholar]
  10. Guarino L. A., Gonzalez M. A., Summers M. D. 1986; Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus. Journal of Virology 60:224–229
    [Google Scholar]
  11. Handermann M., Schnitzler P., Rösen-Wolff A., Raab K., Sonntag K. C., Darai G. 1992; Identification and mapping of origins of DNA replication within the DNA sequences of the genome of insect iridescent virus type 6. Virus Genes 6:19–32
    [Google Scholar]
  12. Hink W. F. 1970; Established insect cell line from the cabbage looper, Trichoplusia ni. Nature, London 226:466–467
    [Google Scholar]
  13. Hoheisel J. D. 1989; A cassette with seven unique restriction sites, including octanucleotide sequences: extension of multiple-cloning-site plasmids. Gene 80:151–154
    [Google Scholar]
  14. Igarashi K., Fawl R., Roller R. J., Roizman B. 1993; Construction and properties of a recombinant herpes simplex virus 1 lacking both S-component origins of DNA synthesis. Journal of Virology 67:2123–2132
    [Google Scholar]
  15. Kitner C. R., Sugden B. 1979; The structure of the termini of Epstein–Barr virus. Cell 17:661–671
    [Google Scholar]
  16. Kool M., Vlak J. M. 1993; The structural and functional organization of the Autographa californica nuclear polyhedrosis virus genome: an overview. Archives of Virology 130:1–16
    [Google Scholar]
  17. Kool M., Voncken J. W., van Lier F. L. J., Tramper J., Vlak J. M. 1991; Detection and analysis of Autographa californica nuclear polyhedrosis virus mutants with defective interfering properties. Virology 183:739–746
    [Google Scholar]
  18. Kool M., van den Berg P. M. M. M., Tramper J., Goldbach R. W., Vlak J. M. 1993; Location of two putative origins of DNA replication of Autographa californica nuclear polyhedrosis virus. Virology 192:94–101
    [Google Scholar]
  19. Kornberg A., Baker T. A. 1992 DNA Replication New York: W. H. Freeman;
    [Google Scholar]
  20. Kuzio J., Faulkner P. 1984; Region of repeated DNA in the genome of Choristoneura fumiferana nuclear polyhedrosis virus. Virology 139:185–188
    [Google Scholar]
  21. Lee H. J., Krell P. J. 1982; Generation and analysis of defective genomes of Autographa californica nuclear polyhedrosis virus. Journal of Virology 66:4339–4347
    [Google Scholar]
  22. Longnecker R., Roizman B. 1986; Clustering of genes dispensable for growth in culture in the S component of the HSV-1 genome. Science 236:573–576
    [Google Scholar]
  23. Maeda S., Majima K. 1990; Molecular cloning and physical mapping of the genome of Bombyx mori nuclear polyhedrosis virus. Journal of General Virology 71:1851–1855
    [Google Scholar]
  24. McClintock J. T., Dougherty E. M. 1988; Restriction mapping of Lymantria dispar nuclear polyhedrosis virus DNA: localization of the polyhedrin gene and identification of four homologous regions. Journal of Virology 69:2303–2312
    [Google Scholar]
  25. Nissen M. S., Friesen P. D. 1989; Molecular analysis of the transcriptional regulatory region of an early baculovirus gene. Journal of Virology 63:493–503
    [Google Scholar]
  26. O’Reilly D. R., Miller L. K., Luckow V. A. 1992 Baculovirus Expression Vectors: A Laboratory Manual New York: W. H. Freeman;
    [Google Scholar]
  27. Pearson M., Bjornson R., Pearson G., Rohrmann G. F. 1992; The Autographa californica baculovirus genome: evidence for multiple replication origins. Science 257:1382–1384
    [Google Scholar]
  28. Polvino-Bodnar M., Orberg P. K., Schaffer P. A. 1987; Herpes simplex virus type 1 oriL is not required for virus replication or for the establishment and reactivation of latent infection in mice. Journal of Virology 61:3528–3535
    [Google Scholar]
  29. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  30. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Smith G. E., Summers M. D. 1978; Analysis of baculovirus genomes with restriction endonucleases. Virology 89:517–527
    [Google Scholar]
  32. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  33. Stow N. D., McMonagle E. C. 1983; Characterization of the TRS/IRS origin of DNA replication of herpes simplex virus type 1. Virology 130:427–438
    [Google Scholar]
  34. Summers M. D., Smith G. E. 1987 A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures Texas Agricultural Experiment Station Bulletin no. 1555;
    [Google Scholar]
  35. Tamanoi F., Stillman B. W. 1983; The origin of adenovirus DNA replication. Current Topics in Microbiology and Immunology 109:75–87
    [Google Scholar]
  36. Theilmann D. A., Stewart S. 1992; Tandemly repeated sequence at the 3′ end of the IE-2 gene of the baculovirus Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus in an enhancer element. Virology 187:97–106
    [Google Scholar]
  37. Tjia S. T., Carstens E. B., Doerfler W. 1979; Infection of Spodoptera frugiperda cells with Autographa californica nuclear polyhedrosis virus. Virology 99:399–409
    [Google Scholar]
  38. Tyndall C., La Mantia G., Thacker C. M., Favoloro J., KameN R. 1981; A region of the polyoma virus genome between the replication origin and late protein coding sequences is required in cis for both early gene expression and viral DNA replication. Nucleic Acids Research 9:6231–6250
    [Google Scholar]
  39. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13:213–217
    [Google Scholar]
  40. Wickham T. J., Davis T., Granados R. R., Hammer D. A., Shuler M. L., Wood H. A. 1991; Baculovirus defective interfering particles are responsible for variations in recombinant protein production as a function of multiplicity of infection. Biotechnology Utters 13:483–188
    [Google Scholar]
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