1887

Abstract

RNA from the Hungarian isolate of poa semilatent virus (PSLV) directed synthesis of 120K, 75K, 25K (coat protein) and 20K polypeptides. translation of PSLV RNA was blocked by the cap analogue, mGpp, thus suggesting that the virus RNA was capped. PSLV RNA could be aminoacylated with [C]tyrosine . The sequence of 1.5 kb from the 3′ end of the PSLV RNA γ component revealed two open reading frames (ORFs) separated by a uridinerich intergenic region. The putative product of the incomplete 5′-proximal ORF showed a close amino acid sequence similarity with the C-terminal segment of the γa protein (putative RNA replicase) encoded in the barley stripe mosaic virus (BSMV) RNA γ, and the 20K product of the 3′-proximal ORF was found to be related to the 17K γb product of BSMV. The sequence of 0.8 kb from the 3′ end of PSLV RNA β encompassed two (incomplete) overlapping ORFs whose putative products are related to the βc and βd proteins encoded in the similarly arranged ORFs of BSMV RNA β. Nucleotide sequence homology between the respective parts of the two hordeivirus genomes was restricted to the ORF for γa, the spacer between the ORFs for γa and γb, and the 3′ non-coding region, particularly the 95 nucleotide segment at the 3′ end representing a tRNA-like structure. Despite limited sequence conservation beyond this segment, the entire 3′ non-coding region of PSLV RNA could be folded in a tight pseudoknotted structure closely resembling that of BSMV RNA. Surprisingly, the ‘signature’ sequence typical for BSMV RNA, internal polydisperse poly(A) intercalated between the coding part and the 3′ tRNA-like structure, was not detected in the PSLV genome. Instead, the virus RNA contained several oligoadenylate stretches spaced by other residues, close to the junction of its coding and 3′ non-coding portions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-73-8-2085
1992-08-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/73/8/JV0730082085.html?itemId=/content/journal/jgv/10.1099/0022-1317-73-8-2085&mimeType=html&fmt=ahah

References

  1. Afanasiev B. N., Rupasov V. V., Fedchenko V. I., Doua V. V., Atabekov J. G., Chernov B. K., Kozlov Y. V., Bayev A. A. 1986; The nucleotide sequence of barley stripe mosaic virus RNA 3 and its relation to RNAs 2b and 4. Doklady Akademii nauk SSSR 290:724–727
    [Google Scholar]
  2. Agranovsky A. A., Dolja V. V., Kagramanova V. K., Atabekov J. G. 1979; The presence of a cap structure at the 5′-end of BSMV RNA. Virology 95:208–210
    [Google Scholar]
  3. Agranovsky A. A., Dolja V. V., Gorbulev V. G., Kozlov Y. V., Atabekov J. G. 1981; Aminoacylation of barley stripe mosaic virus RNA: polyadenylate-containing RNA has a 3′-terminal tyrosine-accepting structure. Virology 113:174–187
    [Google Scholar]
  4. Agranovsky A. A., Dolja V. V., Atabekov J. G. 1982; Structure of the 3′-extremity of barley stripe mosaic virus RNA: evidence for internal poly(A) and a 3′-terminal tRNA-like structure. Virology 119:51–58
    [Google Scholar]
  5. Agranovsky A. A., Dolja V. V., Atabekov J. G. 1983; Differences in polyadenylate length between individual barley stripe mosaic virus RNA species. Virology 129:344–349
    [Google Scholar]
  6. Atabekov J. G., Dolja V. V. 1986; Hordeiviruses: structure and replication. In The Plant Viruses vol 2 The Rod-Shaped Plant Viruses pp 397–420 Edited by van Regenmortel M. H. V., Fraenkel-Conrat H. New York: Plenum Press;
    [Google Scholar]
  7. Atabekov J. G., Novikov V. K. 1989; Barley stripe mosaic virus. CM1/AAB Descriptions of Plant Viruses no. 344
    [Google Scholar]
  8. Brodsky L. I., Drachev A. L., Tatuzov R. L., Chumakov K. M. 1991; GENEBEE: a package of computer programs for biopolymer sequence analysis. Biopolimery i Kletka (Moscow) 7:10–14
    [Google Scholar]
  9. Corpet F. 1988; Multiple sequence alignment with hierarchical clustering. Nucleic Acids Research 16:10881–10890
    [Google Scholar]
  10. Dolja V. V., Agranovsky A. A., Lunina N. A., Atabekov J. G. 1983a; Short virion RNA in barley stripe mosaic virus. FEBS Letters 151:215–218
    [Google Scholar]
  11. Dolja V. V., Lunina N. A., Leiser R.-M., Stanarius T., Belzelarskaya S. N., Kozlov Yu. V., Atabekov J. G. 1983b; A comparative study of the in vitro translation products of individual RNAs from two–, three– and four–component strains of barley stripe mosaic virus. Virology 127:1–14
    [Google Scholar]
  12. England T. E., Uhlenbeck O. C. 1978; 3′-Terminal labelling of RNA with T4 RNA ligase. Nature, London 275:560–561
    [Google Scholar]
  13. Feinberg A. P., Vogelstein B. 1983; A technique for radiolabeling DNA restriction fragments to high specific activity. Analytical Biochemistry 132:6–13
    [Google Scholar]
  14. Gubler U. 1988; A one tube reaction for the synthesis of blunt-ended double-stranded cDNA. Nucleic Acids Research 16:2726
    [Google Scholar]
  15. Gustafson G., Armour S. L. 1986; The complete nucleotide sequence of RNA β from the type strain of barley stripe mosaic virus. Nucleic Acids Research 14:3895–3909
    [Google Scholar]
  16. Gustafson G., Larkins B. A., Jackson A. O. 1981; Comparative analysis of polypeptides synthesized in vivo and in vitro by two strains of barley stripe mosaic virus. Virology 111:579–586
    [Google Scholar]
  17. Gustafson G., Hunter B., Hanau R., Armour S. L., Jackson A. O. 1987; Nucleotide sequence and genetic organization of barley stripe mosaic virus RNA y . Virology 158:394–406
    [Google Scholar]
  18. Gustafson G., Armour S. L., Gamboa G. C., Burgett S. G., Shepherd J. W. 1989; Nucleotide sequence of barley stripe mosaic virus RNA α: RNA α encodes a single polypeptide with homology to corresponding proteins from other viruses and to the BSMV γ protein. Virology 170:370–377
    [Google Scholar]
  19. Hall T. C. 1979; Transfer RNA-like structures in viral genomes. International Review of Cytology 60:1–26
    [Google Scholar]
  20. Hunter B. G., Heaton L. A., Bracker C. E., Jackson A. O. 1986; Structural comparison of poa semilatent virus and barley stripe mosaic virus. Phytopathology 76:322–326
    [Google Scholar]
  21. Hunter B. G., Smith J., Fattouh F., Jackson A. O. 1989; Relationship of lychnis ringspot virus to barley stripe mosaic virus and poa semilatent virus. Intervirology 30:18–26
    [Google Scholar]
  22. Jackson A. O., Dawson J. R. O., Covey S. N., Hull R., Davies J. W., McFarland J. E., Gustafson G. D. 1983; Sequence relations and coding properties of a subgenomic RNA isolated from barley stripe mosaic virus. Virology 127:37–44
    [Google Scholar]
  23. Jackson A. O., Hunter B. G., Gustafson G. D. 1989; Hordeivirus relationships and genome organization. Annual Review of Phytopathology 27:95–121
    [Google Scholar]
  24. Karasev A. V., Alexandrova N. M., Miroshnichenko N. A., Nesterova I. V., Dorokhov Yu. L. 1987; Translational activity of messenger RNAs from potato virus X–infected plants. Moleku-laymaya Biologiaya (Moscow) 21:1367–1377
    [Google Scholar]
  25. Koonin E. V., Boyko V. P., Dolja V. V. 1991; Small cysteine-rich proteins of different groups of plant RNA viruses are related to different families of nucleic acid-binding proteins. Virology 181:395–398
    [Google Scholar]
  26. Kozlov Y. V., Rupasov V. V., Adyshev V. D., Belgelarskaya S. N., Agranovsky A. A., Mankin A. S., Morozov S. Y., Dolja V. V., Atabekov J. G. 1984; Nucleotide sequence of the 3′-terminal tRNA-like structure in barley stripe mosaic virus genome. Nucleic Acids Research 12:4001–4009
    [Google Scholar]
  27. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 27:680–685
    [Google Scholar]
  28. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Peattie D. A. 1979; Direct chemical method for sequencing RNA. Proceedings of the National Academy of Sciences, U.S.A. 76:1760–1764
    [Google Scholar]
  30. Pleij C. W. A., Abrahams J. P., van Belkum A., Rietveld K., Bosch L. 1987; The spatial folding of the 3′ non-coding region of aminoacylatable plant viral RNAs. In Positive Strand RNA Viruses pp 299–316 Edited by Brinton M. A., Ruekert R. R. New York: Alan R. Liss;
    [Google Scholar]
  31. Robinson D. J., Mayo M. A., Fritsch C., Jones A. T., Raschke J. H. 1983; Origin and messenger activity of two small RNA species found in particles of tobacco rattle virus strain SYM. Journal of Genera! Virology 64:1591–1599
    [Google Scholar]
  32. Shellness G. S., Williams D. L. 1985; Secondary structure analysis of apolipoprotein II mRNA using enzymatic probes and reverse transcriptase. Evaluation of primer extension for high resolution mapping of mRNA. Journal of Biological Chemistry 260:8637–8646
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-73-8-2085
Loading
/content/journal/jgv/10.1099/0022-1317-73-8-2085
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error