1887

Abstract

Persistence and expression of polydnavirus (MdPDV) was examined in parasitized and virus-injected larvae. Viral DNA persisted in larvae for 6 days, but no increase in the amount of viral DNA present was detected. Viral transcripts were observed in parasitized and virus-injected larvae 4 h post-parasitism and expression continued for 6 days. When specific host tissues were examined, more viral DNA and RNA was detected in haemocytes than in the gut, nervous system and fat body. P-labelled MdPDV DNA hybridized to approximately six different size classes of mRNAs on Northern blots of RNA from haemocytes of parasitized larvae. MdPDV transcription was first detected in haemocytes at 4 h post-parasitism and continued for 6 days. Similar transcripts were observed in haemocytes from larvae that had been injected with calyx fluid or MdPDV plus venom. First-strand cDNA probes of haemocyte-specific MdPDV transcripts hybridized to only certain MdPDV viral DNAs, suggesting that only part of the MdPDV genome is expressed in this host cell type.

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1992-07-01
2021-10-17
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References

  1. Blissard G. W., Vinson S. B., Summers M. D. 1986; Identification, mapping and in vitro translation of Campoletis sonorensis virus mRNAs from parasitized Heliothis virescens larvae. Journal of Virology 57:318–327
    [Google Scholar]
  2. Blissard G. W., Theilmann D. A., Summers M. D. 1989; Segment W of Campoletis sonorensis virus: expression, gene products, and organization. Virology 169:78–89
    [Google Scholar]
  3. Davies D. H., Vinson S. B. 1986; Passive evasion by eggs of the braconid parasitoid Cardiochiles nigriceps of encapsulation in vitro by hemocytes of host Heliothis virescens. Journal of Insect Physiology 32:1003–1010
    [Google Scholar]
  4. Davies D. H., Strand M. R., Vinson S. B. 1987; Changes in differential hemocyte count and in vitro behavior of plasmatocytes from host Heliothis virescens caused by Campoletis sonorensis polydnavirus. Journal of Insect Physiology 33:143–153
    [Google Scholar]
  5. Dover B. A., Davies D. H., Strand M. R., Gray R. S., Keeley L. L., Vinson S. B. 1987; Ecdysteroid-titre reduction and developmental arrest of last instar Heliothis virescens larvae by calyx fluid from the parasitoid Campoletis sonorensis. Journal of Insect Physiology 33:333–338
    [Google Scholar]
  6. Edson K. M., Vinson S. B., Stoltz D. B., Summers M. D. 1981; Virus in a parasitoid wasp: suppression of the cellular immune response in the parasitoid’s host. Science 211:582–583
    [Google Scholar]
  7. Feddersen I., Sanders K., Schmidt O. 1986; Virus-like particle with host protein-like antigenic determinants protect an insect parasitoid from encapsulation. Experientia 42:1278–1280
    [Google Scholar]
  8. Fleming J. G. W., Summers M. D. 1986; Campoletis sonorensis endoparasitic wasps contain forms of Campoletis sonorensis virus DNA suggestive of integrated and extrachromosomal polydnavirus DNAs. Journal of Virology 57:552–562
    [Google Scholar]
  9. Fleming J. G. W., Summers M. D. 1991; Polydnavirus DNA is integrated in the DNA of its parasitoid wasp host. Proceedings of the National Academy of Sciences, U.S.A 88:9770–9774
    [Google Scholar]
  10. Fleming J. G. W., Blissard G. W., Summers M. D. 1983; Expression of Campoletis sonorensis virus in the parasitized host, Heliothis virescens. Journal of Virology 48:74–78
    [Google Scholar]
  11. Guzo D., Stoltz D. B. 1987; Observations on cellular immunity and parasitism in the tussock moth. Journal of Insect Physiology 33:19–31
    [Google Scholar]
  12. Kafatos F. C., Jones C. W., Efstradiadis A. 1979; Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Research 61541–1552
    [Google Scholar]
  13. Kitano H. 1982; Effect of the venom in the gregarious parasitoid Apanteles glomeratus on hemocytic encapsulation by the host, Pieris. Journal of Invertebrate Pathology 40:61–67
    [Google Scholar]
  14. Lackie A. M. 1988; Hemocyte behaviour. Advances in Insect Physiology 21:85–178
    [Google Scholar]
  15. Pringle J. W. H. 1938; Proprioception in insects. Journal of Experimental Biology 15:101–103
    [Google Scholar]
  16. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  17. Rizki R. M., Rizki T. M. 1984; Selective destruction of a host blood cell type by a parasitoid wasp. Proceedings of the National Academy of Sciences, U.S.A 81:6154–6158
    [Google Scholar]
  18. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Salt G. 1968; The resistance of insect parasitoids to the defense reactions of their hosts. Biological Reviews 13:381–495
    [Google Scholar]
  20. Stoltz D. B. 1990; Evidence for chromosomal transmission of polydnavirus DNA. Journal of General Virology 71:1051–1056
    [Google Scholar]
  21. Stoltz D. B., Vinson S. B. 1979; Viruses and parasitism in insects. Advances in Virus Research 24:125–171
    [Google Scholar]
  22. Stoltz D. B., Krell P., Summers M. D., Vinson S. B. 1984; Polydnaviridae – a proposed new family of insect viruses with segmented, double-stranded, circular DNA genomes. Intervirology 21:1–4
    [Google Scholar]
  23. Stoltz D. B., Guzo D., Cook D. 1986; Studies on polydnavirus transmission. Virology 69:903–907
    [Google Scholar]
  24. Stoltz D. B., Guzo D., Belland E. R., Lucarotti C. J., MacKinnon E. A. 1988; Venom promotes uncoating in vitro and persistence in vivo of DNA from a braconid polydnavirus. Journal of General Virology 69:903–907
    [Google Scholar]
  25. Strand M. R. 1990; Characterization of larval development in Pseudoplusia includens (Walker) (Lepidoptera: Noctuidae). Annals of the Entomological Society of America S3:538–544
    [Google Scholar]
  26. Strand M. R., Dover B. A. 1991; Developmental disruption of Pseudoplusia includens and Heliothis virescens larvae by the polydnavirus and venom of Microplitis demolitor. Archives of Insect Biochemistry and Physiology 18:131–145
    [Google Scholar]
  27. Strand M. R., Noda T. 1991; Alterations in the hemocytes of Pseudoplusia includens after parasitism by Microplitis demolitor. Journal of Insect Physiology 37:839–850
    [Google Scholar]
  28. Strand M. R., Wong E. A. 1991; The growth and role of Microplitis demolitor teratocytes in parasitism of Pseudoplusia includens. Journal of Insect Physiology 37:503–515
    [Google Scholar]
  29. Strand M. R., Meola S. M., Vinson S. B. 1986; Correlating pathological symptoms in Heliothis virescens eggs with development of the parasitoid Telenomus heliothidis. Journal of Insect Physiology 32:389–402
    [Google Scholar]
  30. Tanaka T. 1987; Morphological changes in hemocytes of the host, Pseudaletia separata, parasitized by Microplitis mediator or Apanteles kariyai. Developmental and Comparative Immunology 11:57–67
    [Google Scholar]
  31. Tanaka T., Agui N., Hiruma K. 1987; The parasitoid Apanteles kariyai inhibits pupation of its host, Pseudaletia separata, via disruption of prothoracicotropic hormone release. Journal of General and Comparative Endocrinology 67:364–374
    [Google Scholar]
  32. Theilmann D. A., Summers M. D. 1986; Molecular analysis of Campoletis sonorensis virus DNA in the lepidopteran host Heliothis virescens. Journal of General Virology 67:1961–1969
    [Google Scholar]
  33. Theilmann D. A., Summers M. D. 1987; Physical analysis of the Campoletis sonorensis virus genome and identification of a family of tandemly repeated elements. Journal of Virology 61:2589–2598
    [Google Scholar]
  34. Vinson S. B. 1990; How parasitoids deal with the immune system of their host: an overview. Archives of Insect Biochemistry and Physiology 13:3–27
    [Google Scholar]
  35. Webb B. A., Summers M. D. 1990; Venom and viral expression products of the endoparasitic wasp Campoletis sonorensis share epitopes and related sequences. Proceedings of the National Academy of Sciences, U.S.A 87:4961–4965
    [Google Scholar]
  36. Xu D., Stoltz D. 1991; Evidence for a chromosomal location of polydnavirus DNA in the ichneumonid parasitoid Hyposoter fugitivus. Journal of Virology 656693–6704
    [Google Scholar]
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