RT Journal Article SR Electronic(1) A1 Strand, M. R. A1 McKenzie, D. I. A1 Grassl, V. A1 Dover, B. A. A1 Aiken, J. M.YR 1992 T1 Persistence and expression of Microplitis demolitor polydnavirus in Pseudoplusia includens JF Journal of General Virology, VO 73 IS 7 SP 1627 OP 1635 DO https://doi.org/10.1099/0022-1317-73-7-1627 PB Microbiology Society, SN 1465-2099, AB Persistence and expression of Microplitis demolitor polydnavirus (MdPDV) was examined in parasitized and virus-injected Pseudoplusia includens larvae. Viral DNA persisted in P. includens larvae for 6 days, but no increase in the amount of viral DNA present was detected. Viral transcripts were observed in parasitized and virus-injected larvae 4 h post-parasitism and expression continued for 6 days. When specific host tissues were examined, more viral DNA and RNA was detected in haemocytes than in the gut, nervous system and fat body. 32P-labelled MdPDV DNA hybridized to approximately six different size classes of mRNAs on Northern blots of RNA from haemocytes of parasitized larvae. MdPDV transcription was first detected in haemocytes at 4 h post-parasitism and continued for 6 days. Similar transcripts were observed in haemocytes from larvae that had been injected with calyx fluid or MdPDV plus venom. First-strand cDNA probes of haemocyte-specific MdPDV transcripts hybridized to only certain MdPDV viral DNAs, suggesting that only part of the MdPDV genome is expressed in this host cell type., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-73-7-1627