1887

Abstract

The complete sequence of the S7 RNA that codes for the major group-specific coat protein, VP7, of African horsesickness virus serotype 4 (AHSV-4) was determined from cDNA analyses and found to be 1179 nucleotides in length. One single open reading frame of 353 codons was observed defining a protein of 38107 with a net charge of -1.5 at neutral pH. Comparison of the AHSV-4 VP7 sequence with that of bluetongue virus serotype 10 revealed an overall similarity of 44%, with the amino- and carboxy-terminal regions exhibiting the greatest levels of homology. In addition, potential secondary structures of the terminal sequences of the S7 RNA segments of AHSV-4 and BTV serotypes 10, 13 and 17 are presented.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-6-1237
1991-06-01
2023-02-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/6/JV0720061237.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-6-1237&mimeType=html&fmt=ahah

References

  1. Anzola J. V., Xu Z., Asamizu T., Nuss D. L. 1987; Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumour virus genome and defective interfering RNAs. Proceedings of the National Academy of Sciences, U.S.A 84:8301–8305
    [Google Scholar]
  2. Bremer C. W. 1976; A gel electrophoretic study of the protein and nucleic acid components of African horsesickness virus. Onderstepoort Journal of Veterinary Research 43:193–200
    [Google Scholar]
  3. Bremer C. W., Huismans H., Van Dijk A. A. 1990; Characterization and cloning of the African horsesickness virus genome. Journal of General Virology 71:793–799
    [Google Scholar]
  4. Desselberger U., Racaniello V. R., Zazra J. J., Palese P. 1980; The 3′- and 5′-terminal sequences of influenza A, B, and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene 8:315–328
    [Google Scholar]
  5. French T. J., Roy P. 1990; Synthesis of bluetongue virus (BTV) corelike particles by a recombinant baculovirus expressing the two major structural core proteins of BTV. Journal of Virology 64:1530–1536
    [Google Scholar]
  6. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences, U.S.A. 72:3961–3965
    [Google Scholar]
  7. Howell P. G. 1962; The isolation and identification of further antigenic types of African horsesickness virus. Onderstepoort Journal of Veterinary Research 29:139–149
    [Google Scholar]
  8. Hsu M.-T., Parvin J. D., Gupta S., Krystal M., Palese P. 1987; The genomic RNAs of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle. Proceedings of the National Academy of Sciences, U,. S,. A 83:2850–2854
    [Google Scholar]
  9. Kowalik T. F., Li J. K.-K. 1989; Sequence analyses and structure predictions of double-stranded RNA segment S1 and VP7 from United States prototype bluetongue virus serotypes 13 and 10. Virology 172:189–195
    [Google Scholar]
  10. Kowalik T. F., Li J. K.-K., Chuang R. Y., Doi R. H., Osburn B. I. 1990; The complete nucleotide and deduced amino acid sequence of the gene encoding the major inner capsid protein, VP7 of US bluetongue virus serotype 17. Nucleic Acids Research 18:5302
    [Google Scholar]
  11. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  12. McIntosch B. M. 1958; Immunological types of horsesickness virus and their significance in immunization. Onderstepoort Journal of Veterinary Research 27:465–538
    [Google Scholar]
  13. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  14. Mertens P. P. C., Sangar D. V. 1985; Analysis of the terminal sequences of the genome sequences of four orbiviruses. Virology 140:55–67
    [Google Scholar]
  15. Oellermann R. A., Els H. J., Erasmus B. J. 1970; Characterization of African horsesickness virus. Archiv für die gesamte Virusforschung 29:163–174
    [Google Scholar]
  16. Oldfield S., Hirasawa T., Roy P. 1991; Sequence conservation of the outer capsid protein, VP5, of bluetongue virus, a contrasting feature from the outer capsid protein VP2. Journal of General Virology 72:449–451
    [Google Scholar]
  17. Purdy M. A., Petre J., Roy P. 1985; Cloning of the bluetongue virus L3 gene. Journal of Virology 51:754–759
    [Google Scholar]
  18. Purdy M. A., Ghiasi H., Rao C. D., Roy P. 1985; Complete sequence of bluetongue virus L2 RNA that codes for the antigen recognized by neutralizing antibodies. Journal of Virology 55:826–839
    [Google Scholar]
  19. Rao C. D., Kiuchi A., Roy P. 1983; Homologous terminal sequences of the genome double-stranded RNAs of bluetongue viruses. Journal of Virology 46:378–383
    [Google Scholar]
  20. Ritter G. D., Roy P. 1988; Genetic relationships of bluetongue virus serotypes isolated from different parts of the world. Virus Research 11:33–47
    [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  22. Thomas C. P., Booth T. F., Roy P. 1990; Synthesis of bluetongue virus-encoded phosphoprotein and formation of inclusion bodies by recombinant baculovirus in insect cells: it binds the single-stranded RNA species. Journal of General Virology 71:2073–2083
    [Google Scholar]
  23. Wilson W. C., Fukusho A., Roy P. 1990; Diagnostic complementary DNA probes for genome segments 2 and 3 of epizootic hemorrhagic disease virus serotype 1. American Journal of Veterinary Research 51:855–860
    [Google Scholar]
  24. Xu Z., Anzola J. V., Nalin C. M., Nuss D. L. 1989; The 3′-terminal sequence of a wound tumor virus transcript can influence conformational and functional properties associated with the 5′-terminus. Virology 170:511–522
    [Google Scholar]
  25. Yu Y., Fukusho A., Ritter G. D., Roy P. 1988; Complete nucleotide sequence of the group-reactive antigen VP7 gene of bluetongue virus. Nucleic Acids Research 16:1620
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-6-1237
Loading
/content/journal/jgv/10.1099/0022-1317-72-6-1237
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error