1887

Abstract

Major immunogenic sites of foot-and-mouth disease virus (FMDV) have been mapped to the C-terminal third of capsid protein VP1; we studied the immunogenicity of a series of TrpE-FMDV fusion proteins containing this region of FMDV strain O1 Campos. Fusion protein TrpE-dCN, which contains a dimer of VP1 amino acid sequences consisting of amino acids 200 to 213 linked by a diproline spacer to amino acids 141 to 158 (200–213 ∼ P-P-G ∼ 141–158), induced the best response. A single inoculation of guinea-pigs with 100 µg TrpE-dCN elicited high levels of neutralizing antibodies and protected all the animals against challenge infection with homologous virus. Although the closely related FMDV strains O1 Campos and O1 Caseros induced high levels of cross-protection, TrpE-dCN-vaccinated guinea-pigs were poorly protected against challenge infection with heterologous FMDV strain O1 Caseros. Nucleotide sequence analysis revealed that amino acid differences at residues 149 and 152 were critical for the induction of cross-protection and that neutralizing epitopes not present in TrpE-dCN are likely to be responsible for conferring a high level of cross-protection between FMDV strains O1 Campos and O1 Caseros.

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1991-04-01
2024-12-04
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References

  1. Abaracon D., Gomes I. 1976; Immune response of cattle after vaccination with inactivated footandmouth disease vaccine of 5 strains of subtype O1 . Boletín del Centro Panamericano de Fiebre Aftosa 23-24:3–10
    [Google Scholar]
  2. Acharya R., Fry E., Stuart D., Fox G., Rowlands D., Brown F. 1989; The three-dimensional structure of foot-and-mouth disease virus at 2.9 A resolution. Nature, London 337:709–716
    [Google Scholar]
  3. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against foot-and-mouth disease by immunization with chemically synthesized peptide predicted from the viral nucleotide sequence. Nature, London 198:30–33
    [Google Scholar]
  4. Broekhuijsen M. P., van Rijn J. M. M., Blom A. J. M., Pouwels P. H., Enger-Valk B. E., Brown F., Francis M. J. 1987; Fusion proteins with multiple copies of the major antigenic determinant of foot-and-mouth disease virus protect both the natural host and laboratory animals. Journal of General Virology 68:3137–3143
    [Google Scholar]
  5. Dieckmann C. L., Tzagoloff A. 1985; Assembly of the mitochondrial system CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b. Journal of Biological Chemistry 260:1513–1520
    [Google Scholar]
  6. DiMarchi R., Brooke G., Gale C., Cracknell V., Doel T., Mowat N. 1986; Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232:639–641
    [Google Scholar]
  7. Doel T. R., Gale C., Brooke G., DiMarchi R. 1988; Immunization against foot-and-mouth disease with synthetic peptides representing the C-terminal region of VP1. Journal of General Virology 69:2403–2406
    [Google Scholar]
  8. Doel T. R., Gale C., Do Amaral C. M. C. F., Mulcahy G., DiMarchi R. 1990; Heterotypic protection induced by synthetic peptides corresponding to three serotypes of foot-and-mouth disease virus. Journal of Virology 64:2260–2264
    [Google Scholar]
  9. Fox G., Parry N. R., Barnett P. V., McGinn B., Rowlands D. J., Brown F. 1989; The cell attachment site on foot-and-mouth disease virus includes the amino acid sequence RGD (arginine-glycine-aspartic acid). Journal of General Virology 70:625–637
    [Google Scholar]
  10. Francis M. J., Fry C. M., Rowlands D. J., Bittle J. L., Houghten R. A., Lerner R. A., Brown F. 1987; Immune response to uncoupled peptides of foot-and-mouth disease virus. Immunology 61:1–6
    [Google Scholar]
  11. Giavedoni L. 1988 Production of FMDV antigens by genetic engineering techniques Ph. D. thesis University of Buenos Aires;
    [Google Scholar]
  12. Kitson J. D. A., McCahon D., Belsham G. J. 1990; Sequence analysis of monoclonal antibody resistant mutants of type O foot- and-mouth disease virus: evidence for the involvement of the three surface exposed capsid proteins in four antigenic sites. Virology 179:26–34
    [Google Scholar]
  13. Kleid D. G., Dowbenko D. J., Bock L. A., Hotlin M. E., Jackson M. L., Patzer E. J., Shine S. J., Weddell G. N., Yansura D. G., Morgan D. O., McKercher P. D., Moore D. M. 1985; Production of recombinant vaccines from microorganisms: vaccine for foot-and-mouth disease. In Microbiology 1985 pp 405–408 Edited by Leive L. Washington D. C.: American Society for Microbiology;
    [Google Scholar]
  14. Kurz C., Forss S., Kuepper H., Strohmaier K., Schaller H. 1981; Nucleotide sequence and corresponding amino acid sequence of the gene for the major antigen of foot-and-mouth disease virus. Nucleic Acids Research 9:1919–1931
    [Google Scholar]
  15. Laporte J., Grosclaude J., Wantyghem J., Bernard S., Rouze P. 1973; Neutralization en culture cellulaire du pouvoir infectieux du virus de la fievre aphteuse par de serums provenant de pores immunises a l’aide d’une proteine virale purifiee. Compte rendu hebdomadaire des seances de I’Academie des sciences D276:3399–3401
    [Google Scholar]
  16. Parry N. R., Barnett P. V., Ouldridge E. J., Rowlands D. J., Brown F. 1989a; Neutralizing epitopes of type O foot-and-mouth disease virus. II. Mapping three conformational sites with synthetic peptide reagents. Journal of General Virology 70:1493–1503
    [Google Scholar]
  17. Parry N. R., Ouldridge E. J., Barnett P. V., Clarke B. E., Francis M. J., Fox J. D., Rowlands D. J., Brown F. 1989b; Serological prospects for peptide vaccines against foot-and-mouth disease virus. Journal of General Virology 70:2919–2930
    [Google Scholar]
  18. Pfaff E., Mussgay M., Bohm H. O., Schulz G. F., Schaller H. 1982; Antibodies against a preselected peptide recognize and neutralize foot-and-mouth disease virus. EMBO Journal 1:869–874
    [Google Scholar]
  19. Piccone M. E., Kaplan G., Giavedoni L., Domingo E., Palma E. L. 1988; VP1 of serotype C foot-and-mouth disease viruses: long-term conservation of sequences. Journal of Virology 62:1469–1473
    [Google Scholar]
  20. Reed L. H., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  21. Skinner H. H. 1953; One-week-old white mice as test animals in foot-and-mouth disease research. In Proceedings of the XVth International Veterinary Congress, Stockholm, Part II pp 208–210
    [Google Scholar]
  22. Spindler K. R., Rosser D. S. E., Berk A. J. 1984; Analysis of adenovirus transforming proteins from early regions 1A and IB with antisera to inducible fusion antigens produced in Escherichia coli . Journal of Virology 49:132–141
    [Google Scholar]
  23. Strohmaier K., Franze R., Adam K.-H. 1982; Location and characterization of the antigenic portion of the FMDV immunizing protein. Journal of General Virology 59:295–306
    [Google Scholar]
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