1887

Abstract

The complete nucleotide sequence of a hepatitis C virus derived from plasma of a human carrier in Japan was determined. The cDNA of the isolate (HC-J6) contained 9481 nucleotides and an additional T stretch of 30 to 108 nucleotides at the 3′ end, and had one large open reading frame coding for a polyprotein of 3033 amino acids. It differed by 31.8 to 32.1% in the nucleotide sequence and by 27.4 to 27.7% in the amino acid sequence from an American isolate and two Japanese isolates previously reported. Among these four isolates, the 5′ non-coding region of 329 to 341 nucleotides was well conserved (>93% identity), whereas the 3′ non-coding region of 39 to 45 nucleotides (T stretches not included) was more variable (>30% identity). An excellent degree of conservation of the 5′ non-coding region would reflect its pivotal role in replication, and primers deduced from this region could be applied for the sensitive and specific detection of viral RNA by polymerase chain reaction. Due to a high degree of similarity in the amino acid sequence of the putative core protein (>90%), antigen probes deduced from it would be suitable for the serological diagnosis of HCV infection. Low sequence similarity in the putative envelope protein (>53% identity), however, would have to be taken into account in considering the immunoprophylaxis of HCV infection.

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1991-11-01
2022-12-01
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References

  1. Alter H. J., Purcell R. H., Shih J. W., Melpolder J. C., Houghton M., Choo Q.-L., Kuo G. 1989; Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. New England Journal of Medicine 321:1494–1500
    [Google Scholar]
  2. Bazan J. F., Fletterick R. J. 1989; Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology 171:637–639
    [Google Scholar]
  3. Buonagurio D. A., Nakada S., Parvin J. D., Krystal M., Palese P., Fitch W. M. 1986; Evolution of human influenza A viruses over 50 years: rapid, uniform rate of change in NS gene. Science 232:980–982
    [Google Scholar]
  4. Chambers T. J., Hahn C. S., Galler R., Rice C. M. 1990; Flavivirus genome organization, expression, and replication. Annual Review of Microbiology 44:649–688
    [Google Scholar]
  5. Choo Q.-L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 244:359–362
    [Google Scholar]
  6. Choo Q.-L., Richman K. H., Han J. H., Berger K., Lee C., Dong C., Gallegos C., Coit D., Medina-Selby A., Barr P. J., Weiner A. J., Bradley D. W., Kuo G., Houghton M. 1991; Genetic organization and diversity of the hepatitis C virus. Proceedings of the National Academy of Sciences, U.S.A. 88:2451–2455
    [Google Scholar]
  7. Collett M. S., Larson R., Gold C., Strick D., Anderson D. K., Purchio A. F. 1988; Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165:191–199
    [Google Scholar]
  8. Dienstag J. L. 1983; Non-A, non-B hepatitis. I. Recognition, epidemiology, and clinical features. Gastroenterology 85:439–462
    [Google Scholar]
  9. Esteban J. I., Esteban R., Viladomiu L., López-Talavera J. C., Gonzalez A., Hernandez J. M., Roget M., Vargas V., Genesca J., Buti M., Guardia J., Houghton M., Choo Q.-L., Kuo G. 1989; Hepatitis C virus antibodies among risk groups in Spain. Lancet ii:294–297
    [Google Scholar]
  10. Feinstone S. M., Kapikian A. Z., Purcell R. H., Alter H. J., Holland P. V. 1975; Transfusion-associated hepatitis not due to viral hepatitis type A or B. New England Journal of Medicine 292:767–770
    [Google Scholar]
  11. Hahn B. H., Shaw G. M., Taylor M. E., Redfield R. R., Markham P. D., Salahuddin S. Z., Wong-Staal F., Gallo R. C., Parks E. S., Parks W. P. 1986; Genetic variation in HTLV-III/LAV over time in patients with AIDS or at risk for AIDS. Science 232:1548–1553
    [Google Scholar]
  12. Han J. H., Shyamala V., Richman K. H., Brauer M. J., Irvine B., Urdea M. S., Tekamp-Olson P., Kuo G., Choo Q.-L., Houghton M. 1991; Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5’ untranslated region and poly(A) tails at the 3′ end. Proceedings of the National Academy of Sciences, U.S.A. 88:1711–1715
    [Google Scholar]
  13. Heinz F. X. 1986; Epitope mapping of flavivirus glycoproteins. Advances in Virus Research 31:103–168
    [Google Scholar]
  14. Kamer G., Argos P. 1984; Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
    [Google Scholar]
  15. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Sugimura T., Shimotohno K. 1990; Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proceedings of the National Academy of Sciences, U.S.A. 87:9524–9528
    [Google Scholar]
  16. Kuo G., Choo Q.-L., Alter H. J., Gitnick G. L., Redeker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E., Tegtmeier G. E., Bonino F., Colombo M., Lee W.-S., Kuo C., Berger K., Shuster J. R., Overby L. R., Bradley D. W., Houghton M. 1989; An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244:362–364
    [Google Scholar]
  17. Laín S., Riechmann J. L., Martín M. T., Garcia J. A. 1989; Homologous potyvirus and flavivirus proteins belonging to a superfamily of helicase-like proteins. Gene 82:357–362
    [Google Scholar]
  18. Meyers G., Rumenapf T., Thiel H.-J. 1989; Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology 171:555–567
    [Google Scholar]
  19. Miller R. H., Purcell R. H. 1990; Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proceedings of the National Academy of Sciences, U.S.A. 87:2057–2061
    [Google Scholar]
  20. Moormann R. J. M., Warmerdam P. A. M., van der Meer B., Schaaper W. M. M., Wensvoort G., Hulst M. M. 1990; Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genomic region encoding envelope protein El. Virology 177:184–198
    [Google Scholar]
  21. Muraiso K., Hijikata M., Ohkoshi S., Cho M.-J., Kikuchi M., Kato N., Shimotohno K. 1990; A structural protein of hepatitis C virus expressed in E. coli facilitates accurate detection of hepatitis C virus. Biochemical and Biophysical Research Communications 172:511–516
    [Google Scholar]
  22. Ohara O., Dorit R. L., Gilbert W. 1989; One-sided polymerase chain reaction: the amplification of cDNA. Proceedings of the National Academy of Sciences, U.S.A. 86:5673–5677
    [Google Scholar]
  23. Okamoto H., Munekata E., Tsuda F., Takahashi K., Yotsumoto S., Tanaka T., Tachibana K., Akahane Y., Sugai Y., Miyakawa Y., Mayumi M. 1990a; Enzyme-linked immunosorbent assay for antibodies against the capsid protein of hepatitis C virus with a synthetic oligopeptide. Japanese Journal of Experimental Medicine 60:223–233
    [Google Scholar]
  24. Okamoto H., Okada S., Sugiyama Y., Tanaka T., Sugai Y., Akahane Y., Machida A., Mishiro S., Yoshizawa H., Miyakawa Y., Mayumi M. 1990b; Detection of hepatitis C virus RNA by a two-stage polymerase chain reaction with two pairs of primers deduced from the 5′-noncoding region. Japanese Journal of Experimental Medicine 60:215–222
    [Google Scholar]
  25. Okamoto H., Okada S., Sugiyama Y., Yotsumoto S., Tanaka T., Yoshizawa H., Tsuda F., Miyakawa Y., Mayumi M. 1990c; The 5′-terminal sequence of the hepatitis C virus genome. Japanese Journal of Experimental Medicine 60:167–177
    [Google Scholar]
  26. Prince A. M., Brotman B., Grady G. F., Kuhns W. J., Hazzi C., Levine R. W., Millian S. J. 1974; Long-incubation post-transfusion hepatitis without serological evidence of exposure to hepatitis-B virus. Lancet ii:241–246
    [Google Scholar]
  27. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  28. Steinhauer D. A., Holland J. J. 1987; Rapid evolution of RNA viruses. Annual Review of Microbiology 41:409–433
    [Google Scholar]
  29. Takamizawa A., Mori C., Fuke I., Manabe S., Murakami S., Fujita J., Onishi E., Andoh T., Yoshida I., Okayama H. 1991; Structure and organization of the hepatitis C virus genome isolated from human carriers. Journal of Virology 65:1105–1113
    [Google Scholar]
  30. Takeuchi K., Kubo Y., Boonmar S., Watanabe Y., Katayama T., Choo Q.-L., Kuo G., Houghton M., Saito I., Miyamura T. 1990; The putative nucleocapsid and envelope protein genes of hepatitis C virus determined by comparison of the nucleotide sequences of two isolates derived from an experimentally infected chimpanzee and healthy human carriers. Journal of General Virology 71:3027–3033
    [Google Scholar]
  31. VAN der Poel C. L., Reesink H. W., Schaasberg W., Leentvaar-Kuypers A., Barker E., Exel-Oehlers P. J., Lelie P. N. 1990; Infectivity of blood seropositive for hepatitis C virus antibodies. Lancet 335:558–560
    [Google Scholar]
  32. van der Poel C. L., Cuypers H. T. M., Reesink H. W., Weiner A. J., Quan S., di Nello R., van Boven J. J. P., Winkel I., Mulder-Folkerts D., Exel-Oehlers P. J., Schaasberg W., Leentvaar-Kuypers A., Polito A., Houghton M., Lelie P. N. 1991; Confirmation of hepatitis C virus infection by new four-antigen recombinant immunoblot assay. Lancet 33 7:317–319
    [Google Scholar]
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