1887

Abstract

The post-translational events leading from the precursor to the processed forms of a glycoprotein with an of 28K to 32K (gp28/32) of Marek's disease virus (MDV) serotype 2 were examined with pulse-chase experiments and treatment with tunicamycin and monensin. Cell-free translation of infected cell mRNA followed by immunoprecipitation analysis suggested that a polypeptide with a size of 22K is the initial precursor. Experiments with endo-β--acetylglucosaminidase H and endo-β--acetylglucosaminidase F indicated that gp28/32 contains mostly -linked oligosaccharides of the complex type. These studies showed that 22K, the initial product, is then processed through intermediates to the 28K to 32K form.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-8-1807
1990-08-01
2019-11-17
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-8-1807
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error