The post-translational events leading from the precursor to the processed forms of a glycoprotein with an of 28K to 32K (gp28/32) of Marek's disease virus (MDV) serotype 2 were examined with pulse-chase experiments and treatment with tunicamycin and monensin. Cell-free translation of infected cell mRNA followed by immunoprecipitation analysis suggested that a polypeptide with a size of 22K is the initial precursor. Experiments with endo-β--acetylglucosaminidase H and endo-β--acetylglucosaminidase F indicated that gp28/32 contains mostly -linked oligosaccharides of the complex type. These studies showed that 22K, the initial product, is then processed through intermediates to the 28K to 32K form.


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