@article{mbs:/content/journal/jgv/10.1099/0022-1317-71-8-1807, author = "Nakajima, Kazuhiro and Shibayama, Takeshi and Kato, Shiro and Kurimura, Takashi and Hirai, Kanji", title = "Synthesis and Processing of a gp28/32 Membrane Glycoprotein Induced by Marek's Disease Virus Serotype 2", journal= "Journal of General Virology", year = "1990", volume = "71", number = "8", pages = "1807-1810", doi = "https://doi.org/10.1099/0022-1317-71-8-1807", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-8-1807", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The post-translational events leading from the precursor to the processed forms of a glycoprotein with an M t of 28K to 32K (gp28/32) of Marek’s disease virus (MDV) serotype 2 were examined with pulse-chase experiments and treatment with tunicamycin and monensin. Cell-free translation of infected cell mRNA followed by immunoprecipitation analysis suggested that a polypeptide with a size of 22K is the initial precursor. Experiments with endo-β-N-acetylglucosa- minidase H and endo-β-N-acetylglucosaminidase F indicated that gp28/32 contains mostly N-linked oligosaccharides of the complex type. These studies showed that 22K, the initial product, is then processed through intermediates to the 28K to 32K form.", }