1887

Abstract

Synthetic peptides P37–49 and P63–79, derived from the pre-S region of duck hepatitis B virus (DHBV), have been synthesized. Only P37–49 was reactive with rabbit anti-DHBs/pre-S antibodies by radioimmuno-precipitation. Antiserum prepared against P37–47 reacted with a 35K polypeptide of native DHBs/pre-S by immunoblotting. It is concluded that P37–49 (MGQHPAKSMDVRR) mimics one of the epitopes of the DHBV pre-S antigen.

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/content/journal/jgv/10.1099/0022-1317-71-10-2467
1990-10-01
2023-01-28
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References

  1. Chou P. Y., Fasman G. D. 1978; Prediction of the secondary structure of proteins from their amino acid sequence. Advances in Enzymology 47:54–147
    [Google Scholar]
  2. Lambert V., Fernhotz D., Sprengel R., Fourel I., Delange G., Wildner G., Peynet C., Trepo C., Cova L., Will H. 1990; Virus neutralizing monoclonal antibody to a conserved epitope on the duck hepatitis B virus pre-S protein. Journal of Virology 64:1290–1297
    [Google Scholar]
  3. Mandart E., Kay A., Galibert F. 1984; Nucleotide sequences of a cloned duck hepatitis B virus genome: comparison with woodchuck and human hepatitis B virus sequences. Journal of Virology 49:782–789
    [Google Scholar]
  4. Merrifield R. B. 1963; Solid phase peptide synthesis. I. The synthesis of a tetrapeptide. Journal of the American Chemical Society 85:2149–2154
    [Google Scholar]
  5. Neurath A, Kent S. B. 1984; Location and chemical synthesis of a pre-S gene coded immunodominant epitope of hepatitis B virus. Science 224:392–394
    [Google Scholar]
  6. Neurath A. R., Kent S.B, Parker K. 1986a; Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell 46:429–436
    [Google Scholar]
  7. Neurath A. R., Adamowicz P., Kent S. B., Riottet N. M., Strick N., Parker K., Offensperger W., Petit M. A., Wahl S., Budkowska A., Girard M., Pillet J. 1986b; Characterization of monoclonal antibodies specific for the pre-S2 region of the hepatitis B virus envelope protein. Molecular Immunology 23:991–997
    [Google Scholar]
  8. Pugh J. C., Sninsky J. J., Summers J. W., Schaeffer E. 1987; Characterization of a pre-S polypeptide on the surfaces of infectious avian hepadnavirus particles. Journal of Virology 61:1384–1390
    [Google Scholar]
  9. Schlicht H. J., Kuhn C., Guhr B., Mattaliano R. J., Schaller H. 1987; Biochemical and immunological characterization of the duck hepatitis B virus envelope proteins. Journal of Virology 61:2280–2285
    [Google Scholar]
  10. Zhang W., Hu C. D., Lo Y. F., Wen Y.-M. 1988; Study of polypeptide patterns of duck hepatitis B surface antigen. Chinese Journal of Microbiology and Immunology 8:258–272
    [Google Scholar]
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