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The 5′ terminus of barley yellow dwarf virus RNA did not become labelled with 32P after dephosphorylation was attempted with calf intestinal alkaline phosphatase and subsequent treatment with [γ-32P]ATP and T4 polynucleotide kinase. Treatment of BYDV RNA with 125I-Bolton-Hunter reagent yielded 125I-labelled BYDV RNA, as shown by acid precipitation analysis. Treatment with RNase yielded a protein of approximate M r 17000 that was destroyed by treatment with Pronase and was serologically distinct from BYDV coat protein. BYDV therefore appears to have a genome-linked protein.
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