1887

Abstract

Summary

The 5' terminus of barley yellow dwarf virus RNA did not become labelled with P after dephosphorylation was attempted with calf intestinal alkaline phosphatase and subsequent treatment with [γ-P]ATP and T4 polynucleotide kinase. Treatment of BYDV RNA with I-Bolton-Hunter reagent yielded I-labelled BYDV RNA, as shown by acid precipitation analysis. Treatment with RNase yielded a protein of approximate 17 000 that was destroyed by treatment with Pronase and was serologically distinct from BYDV coat protein. BYDV therefore appears to have a genome-linked protein.

Keyword(s): BYDV and genome-linked protein
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/content/journal/jgv/10.1099/0022-1317-70-8-2253
1989-08-01
2019-11-12
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-8-2253
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