1887

Abstract

SUMMARY

The nucleotide sequence of the 3′-proximal 2630 nucleotides of potato virus M (PVM) genomic RNA was determined. The sequenced region contained five long open reading frames (ORFs). The ORF nearest to the 3′-terminal poly(A) tail corresponds to a polypeptide of 10848. This ORF is preceded by one which encodes a protein of 33906 (34K) which has an amino acid sequence that is very similar in its carboxyterminal part to that of the coat proteins of some potexviruses. Three other ORFs encoding polypeptides of 24615, 11893 and 6739 are present in the region 5′ to the 34K protein gene. There is extensive similarity between these proteins and the corresponding proteins encoded by the conserved triple gene block in the RNA of potexviruses.

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1989-07-01
2024-05-13
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References

  1. Abouhaidar M. G. 1988; Nucleotide sequence of the capsid protein gene and 3′ non-coding region of papaya mosaic virus RNA. Journal of General Virology 69:219–226
    [Google Scholar]
  2. Angenent G. C., Linthorst H. J. M., Van Belkum A. F., Cornelissen B. I. C., Bol J. F. 1986; RNA 2 of tobacco rattle virus strain TCM encodes an unexpected gene. Nucleic Acids Research 14:4673–4682
    [Google Scholar]
  3. Bendena W. G., Bancroft J. B., Mackie G. A. 1987; Molecular cloning of clover yellow mosaic virus RNA. Identification of coat protein sequences in vivo and in vitro. Virology 157:276–284
    [Google Scholar]
  4. Bouzoubaa S., Ziegler V., Beck D., Guilley H., Richards K., Jonard G. 1986; Nucleotide sequence of beet necrotic yellow vein virus RNA 2. Journal of General Virology 67:1689–1700
    [Google Scholar]
  5. Bundin V. S., Vishnyakova O. A., Zakharyev V. M., Morozov S. YU., Atabekov J. G., Skryabin K. G. 1986; Comparative studies of potexvirus genomes: homology between the primary structure of coat protein genes. Dokladȳ Akademii nauk SSSR 290:728–733 in Russian
    [Google Scholar]
  6. De Groot R. J., Andeweg A. C., Horzinek M. C., Spaan W. J. M. 1988; Sequence analysis of the 3′ end of the feline coronavirus FIPV 79-1146 genome: comparison with the genome of porcine coronavirus TGEV reveals large insertions. Virology 167:370–376
    [Google Scholar]
  7. Dolja V. V., Grama D. P., Morozov S. YU., Atabekov J. G. 1987; Potato virus X-related single- and double-stranded RNA. Characterization of terminal structures. FEBS Letters 214:308–312
    [Google Scholar]
  8. Eisenberg D., Schwarz E., Komoromy M., Wall R. 1984; Analysis of membrane and surface protein sequences with the hydrophobic moment plot. Journal of Molecular Biology 179:125–142
    [Google Scholar]
  9. Englund T. E., Bruce A. G., Uhlenbeck O. C. 1980; Specific labeling of 3′ termini of RNA with T4 RNA ligase. Methods in Enzymology 65:65–74
    [Google Scholar]
  10. Forster R. L. S., Bevan M. W., Harbison S.-A., Gardner R. C. 1988; The complete nucleotide sequence of the potexvirus white clover mosaic virus. Nucleic Acids Research 16:291–303
    [Google Scholar]
  11. Gorbalenya A. E., Koonin E. V., Donchenko A. P., Blinov V. M. 1988; A novel superfamily of nucleoside triphosphate-binding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination. FEBS Letters 235:16–24
    [Google Scholar]
  12. Gordon K., Pfeiffer P., Fütterer J., Hohn T. 1988; In vitro expression of cauliflower mosaic virus genes. EMBO Journal 7:309–317
    [Google Scholar]
  13. Grunstein M., Hogness D. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of SciencesU.S.A 72:3961–3965
    [Google Scholar]
  14. Gubler H., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  15. Guilford P. J., Forster R. L. S. 1986; Detection of polyadenylated subgenomic RNAs in leaves infected with the potexvirus daphne virus X. Journal of General Virology 67:83–90
    [Google Scholar]
  16. Gustafson G. D., Armour S. L. 1986; The complete nucleotide sequence of RNA β from the type strain of barley stripe mosaic virus. Nucleic Acids Research 14:3895–3909
    [Google Scholar]
  17. Harbison S.-A., Forster R. L. S., Guilford P. J., Gardner R. C. 1988; Organization and interviral homologies of the coat protein gene of white clover mosaic virus. Virology 162:459–465
    [Google Scholar]
  18. Hiebert S. W., Richardson S. D., Lamb R. A. 1988; Cell surface expression and orientation in membranes of the 44-amino acid SH protein of simian virus 5. Journal of Virology 62:2347–2357
    [Google Scholar]
  19. Hodgman T. C. 1988; A new superfamily of replicative proteins. Nature, London 333:22–23
    [Google Scholar]
  20. Huisman M. J., Linthorst H. J. M., Bol J. F., Cornelissen B. J. C. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
    [Google Scholar]
  21. Hunter T. R., Hunt T., Knowland J., Zimmern D. 1976; Messenger RNA for the coat protein of tobacco mosaic virus. Nature, London 260:759–764
    [Google Scholar]
  22. Klug A., Rhodes D. 1987; ‘Zinc fingers’: a novel protein motif for nucleic acid recognition. Trends in Biochemical Sciences 12:464–469
    [Google Scholar]
  23. Lain S., Reichmann L., Mendez E., Garcia J. A. 1988; Nucleotide sequence of the 3′-terminal region of plum pox potyvirus RNA. Virus Research 10:325–342
    [Google Scholar]
  24. Lamb R. A., Zebedee S. L., Richardson C. D. 1985; Influenza virus M2 protein is an integral membrane protein expressed on the infected cell surface. Cell 40:627–633
    [Google Scholar]
  25. Mackie C. A., Johnston R., Bancroft J. B. 1988; Single- and double-stranded viral RNAs in plants infected with the potexvirus papaya mosaic virus and foxtail mosaic virus. Intervirology 29:170–177
    [Google Scholar]
  26. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  27. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  28. Morozov S. YU., Zakharyev V. M., Chernov B. K., Prasolov V. S., Kozlov YU. V., Atabekov J. G., Skryabin K. G. 1983; The primary structure and the localization of the coat protein gene in the genomic RNA of potato virus X. Dokladӯ Akademii nauk SSSR 271:211–215 in Russian
    [Google Scholar]
  29. Morozov S. YU., Lukasheva L. I., Chernov B. K., Skryabin K. G., Atabekov J. G. 1987; Nucleotide sequence of the open reading frames adjacent to the coat protein cistron in the potato virus X genome. FEBS Letters 213:438–142
    [Google Scholar]
  30. Morozov S. YU., Dolja V. V., Atabekov J. G. 1989; Probable reassortment of genomic elements among elongated RNA-containing plant viruses. Journal of Molecular Evolution in press
    [Google Scholar]
  31. Proll E., Leiser R. M., Ostermann W. D., Spaar D. 1981; Some physicochemical properties of potato virus M. Potato Research 24:1–10
    [Google Scholar]
  32. Rice C. M., Lenches E. M., Eddy S. R., Shin S. J., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229:726–733
    [Google Scholar]
  33. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of SciencesU.S.A. 74:5463–5467
    [Google Scholar]
  34. Sehnke P. C., Mason A. M., Hood S. J., Lister R. M., Johnson J. E. 1989; A ‘zinc-finger’-type binding domain in tobacco streak virus coat protein. Virology 168:48–56
    [Google Scholar]
  35. Short M. N., Turner D. S., Marsch J. F., Pappin D. J. C, Parente A., Davies J. W. 1986; The primary structure of papaya mosaic virus coat protein. Virology 152:280–283
    [Google Scholar]
  36. Skryabin K. G., Morozov S. YU., Kraev A. S., Rozanov M. N., Chernov B. K., Lukasheva L. I., Atabekov J. G. 1988a; Conserved and variable elements in RNA genomes of potexviruses. FEBS Letters 240:33–40
    [Google Scholar]
  37. Skryabin K. G., Kraev A. S., Morozov S. YU., Rozanov M. N., Chernov B. K., Lukasheva L. I., Atabekov J. G. 1988b; The nucleotide sequence of potato virus X RNA. Nucleic Acids Research 16:10929–10930
    [Google Scholar]
  38. Szybiak U., Legocki A. B. 1981; Translation in vitro of ribonucleic acids from potato virus X, potato virus M and white clover mosaic virus. Acta biochimica polonica 28:99–104
    [Google Scholar]
  39. Tavantzis S. M. 1984; Physicochemical properties of potato virus M. Virology 133:427–430
    [Google Scholar]
  40. Valle R. P. C., Morch M.-D. 1988; Stop making sense. Regulation at the level of termination in eukaryotic protein synthesis. FEBS Letters 235:1–15
    [Google Scholar]
  41. Wetter C., Milne R. G. 1981; Carlaviruses. In Plant Virus Infections: Comparative Diagnosis695–730 Kurstak E. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
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