1887

Abstract

SUMMARY

VP-2b, a major structural protein of infectious bursal disease virus (IBDV), and its precursor protein VP-2a were separated in a soluble form from the supernatant of ultracentrifuged viruses by using a monoclonal antibody specific for VP-3, the other major structural protein, to remove soluble VP-3 and remaining virus particles. The native VP-2a/2b inhibited the majority of virus-neutralizing (VN) activity in chicken anti-IBDV sera and chickens immunized with VP-2a/2b produced VN antibodies that passively protected susceptible chickens from infection. However, the separated VP2a/2b was not as immunogenic as intact virus particles. VP-2a/2b would appear to contain a conformational epitope which is destroyed by SDS and boiling and which may prove to be of critical importance in a subunit vaccine against type 1 IBDV,

Keyword(s): IBDV , immunogen and vaccine development
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1989-06-01
2021-10-21
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References

  1. Azad A. A., Jagadish M. N., Brown M. A., Hudson P. J. 1987; Deletion mapping and expression in Escherichia coli of the large genomic segment of a birnavirus. Virology 161:145–152
    [Google Scholar]
  2. Becht H. 1980; Infectious bursal disease virus. Current Topics in Microbiology and Immunology 90:107–121
    [Google Scholar]
  3. Becht H., Müller H., Müller H. K. 1988; Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. Journal of General Virology 69:631–640
    [Google Scholar]
  4. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature, London 298:30–33
    [Google Scholar]
  5. Burnette W. N. 1981; “Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  6. Cohen G. H., Dietzschold B., Ponce De Leon M., Long D., Golub E., Varrichio A., Pereira L., Eisenberg R. J. 1984; Localization and synthesis of an antigenic determinant of herpes simplex virus glycoprotein D that stimulates the production of neutralizing antibody. Journal of Virology 49:102–108
    [Google Scholar]
  7. Cohen G. H., Isola V. J., Kuhns J., Berman P. W., Eisenberg R. J. 1986; Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of a nondenaturing (“native” gel) system of polyacrylamide gel electrophoresis coupled with Western blotting. Journal of Virology 60:157–166
    [Google Scholar]
  8. Dobos P. 1979; Peptide map comparison of the proteins of infectious bursal disease virus. Journal of Virology 32:1046–1050
    [Google Scholar]
  9. Dyall-Smith M. L., Lazdins I., Tregear G. W., Holmes I. H. 1986; Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proceedings of the National Academy of Sciences, U.S.A 83:3465–3468
    [Google Scholar]
  10. Fahey K. J., O’donnell I. J., Azad A. A. 1985a; Characterization by Western blotting of the immunogens of infectious bursal disease virus. Journal of General Virology 66:1479–1488
    [Google Scholar]
  11. Fahey K. J., O’donnell I. J., Bagust T. J. 1985b; Antibody to the 32K structural protein of infectious bursal disease virus neutralizes viral infectivity in vitro and confers protection on young chickens. Journal of General Virology 66:2693–2702
    [Google Scholar]
  12. Hudson P. J., Mckern N. M., Power B. E., Azad A. A. 1986; Genomic structure of the large RNA segment of infectious bursal disease virus. Nucleic Acids Research 14:5001–5012
    [Google Scholar]
  13. Jiménez G., Correa I., Melgosa M. P., Bullido M. J., Enjuanes L. 1986; Critical epitopes in transmissible gastroenteritis virus neutralization. Journal of Virology 60:131–139
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 111:680–685
    [Google Scholar]
  15. Lerner R. A. 1982; Tapping of the immunological repertoire to produce antibodies of predetermined specificity. Nature, London 299:592–596
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  17. Müller H., Becht H. 1982; Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious virus and incomplete particles. Journal of Virology 44:384–392
    [Google Scholar]
  18. Samson A. C. R., Willcocks M. M., Routledge E. G., Morgan L. A., Toms G. L. 1986; A neutralizing monoclonal antibody to respiratory syncytial virus which binds to both F1 and F2 components of the fusion protein. Journal of General Virology 67:1479–1483
    [Google Scholar]
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