@article{mbs:/content/journal/jgv/10.1099/0022-1317-70-6-1473, author = "Fahey, Kevin J. and Erny, Katrina and Crooks, Jennifer", title = "A Conformational Immunogen on VP-2 of Infectious Bursal Disease Virus that Induces Virus-neutralizing Antibodies that Passively Protect Chickens", journal= "Journal of General Virology", year = "1989", volume = "70", number = "6", pages = "1473-1481", doi = "https://doi.org/10.1099/0022-1317-70-6-1473", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-70-6-1473", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "immunogen", keywords = "IBDV", keywords = "vaccine development", abstract = "SUMMARY VP-2b, a major structural protein of infectious bursal disease virus (IBDV), and its precursor protein VP-2a were separated in a soluble form from the supernatant of ultracentrifuged viruses by using a monoclonal antibody specific for VP-3, the other major structural protein, to remove soluble VP-3 and remaining virus particles. The native VP-2a/2b inhibited the majority of virus-neutralizing (VN) activity in chicken anti-IBDV sera and chickens immunized with VP-2a/2b produced VN antibodies that passively protected susceptible chickens from infection. However, the separated VP2a/2b was not as immunogenic as intact virus particles. VP-2a/2b would appear to contain a conformational epitope which is destroyed by SDS and boiling and which may prove to be of critical importance in a subunit vaccine against type 1 IBDV,", }