1887

Abstract

Summary

5′ deletion mutants of the nuclear polyhedrosis virus very late p 10 gene promoter have been prepared and subjected to a transient expression assay in infected cells. The control plasmid contained the chloramphenicol acetyltransferase (CAT) reporter gene under the control of the p 10 promoter, which was included in a 230 bp sequence upstream from the p 10 translation initiation codon. The control plasmid also contained a segment of the hr enhancer downstream from the CAT gene. Promoter activity was unaffected by 5′ deletion to position –77, which lies about 11 bp upstream from the p10 cap site. However, deletion of 12 more bp completely eliminated p10 promoter activity. Thus, the 5′ border of the p10 promoter lies downstream from position –77, and the region between positions –77 and –65 contains an element that is important to promoter activity. This is the region that is conserved near the cap sites of late baculovirus genes. Our studies also show that transient expression of CAT under the control of the p10 promoter and hr enhancer is higher when transfection occurs prior to infection by virus.

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1989-05-01
2021-10-18
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