1887

Abstract

Summary

Using hybridization techniques for viral RNA and employing a specific riboprobe, we have detected virus in neural cells of monkeys infected with poliovirus type 1 (PV-1) by the intraspinal route. In monkeys paralysed after inoculation of a neurovirulent revertant of PV-1/Sabin strain, viral RNA was detected in motor neurons and their processes, and in polymorphonuclear and small neural cells. Quantitative hybridization provided evidence of viral replication in individual cells suggesting that the death of motor neurons was due to the direct effect of poliovirus replication in these cells. The histological study of neural lesions of monkeys paralysed after infection with the attenuated Sabin strain of PV-1 revealed two major differences compared to monkeys infected with a virulent strain: (i) the number of destroyed motor neurons was reduced and limited to the site of inoculation and (ii) the inflammatory reaction was localized but more intense. An account is given of the difference in histopathology induced by virulent and attenuated strains of PV-1 in the central nervous system.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-70-11-2907
1989-11-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/70/11/JV0700112907.html?itemId=/content/journal/jgv/10.1099/0022-1317-70-11-2907&mimeType=html&fmt=ahah

References

  1. Baltimore D., Girard M., Darnell J. E. 1966; Aspects of the synthesis of poliovirus RNA and the formation of viral particles. Virology 29179–189
    [Google Scholar]
  2. Beswick T. S. L., Coid C. R. 1961; The influence of technique on the sensitivity of monkeys to intraspinally inoculated poliovirus. Journal of Hygiene 59:395–399
    [Google Scholar]
  3. Bijlenga G., Heaney T. 1978; Post-exposure local treatment of mice infected with rabies with two axonal flow inhibitors, colchicine and vinblastine. Journal of General Virology 39:381–385
    [Google Scholar]
  4. Blinzinger K., Simon J., Magrath D., Boulger L. 1969; Poliovirus crystal within the endoplasmic reticulum of endothelial and mononuclear cell in the monkey spinal cord. Science 163:1336–1337
    [Google Scholar]
  5. Bodian D. 1949; Histopathologic basis of clinical findings in poliomyelitis. American Journal of Medicine 6:563–578
    [Google Scholar]
  6. Bodian D. 1955; Emerging concept of poliomyelitis infection. Science 122:105–108
    [Google Scholar]
  7. Bodian D. 1964; An electron microscopic study of the monkey spinal cord. Bulletin of the Johns Hopkins Hospital 1:13–19
    [Google Scholar]
  8. Boulger L. R. 1973; The neurovirulence test for live poliomyelitis vaccine. Journal of Biological Standardization 1:119–138
    [Google Scholar]
  9. Brahic M., Haase A. T. 1978; Detection of viral sequences of low reiteration frequency by in situ hybridization. Proceedings of the National Academy of SciencesU.S.A 756125–6129
    [Google Scholar]
  10. Brahic M., Haase A. T., Cash E. 1984; Simultaneous in situ detection of viral RNA and antigens. Proceedings of the National Academy of SciencesU.S.A 815445–5448
    [Google Scholar]
  11. Butler E. T., Chamberlin M. J. 1982; Bacteriophage SP6-specific RNA polymerase. Journal of Biological Chemistry 257:5772–5778
    [Google Scholar]
  12. Couderc T., Guinguene B., Horaud F., Aubert-combiescu A., Crainic R. 1989a; Molecular pathogenesis of type 2 poliovirus in mice. European Journal of Epidemiology
    [Google Scholar]
  13. Couderc T., Barzu T., Horaud F., Crainic R. 1989b; Poliovirus permissivity and specific receptor expression on human endothelial cells. Virology
    [Google Scholar]
  14. Cova L., Kopecka H., Aymard M., Girard M. 1988; Use of cRNA probes for the detection of enteroviruses by molecular hybridization. Journal of Medical Virology 24:11–18
    [Google Scholar]
  15. Cox L. K. H., De Leon D. V., De angerer L. M., Angerer R. C. 1984; Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probe. Developmental Biology 101:485–502
    [Google Scholar]
  16. Dal canto M. C., Barbano R. L., Jubelt B. 1986; Ultrastructural immunohistochemical localization of poliovirus during virulent infection of mice. Journal of Neuropathology and Experimental Neurology 45:613–618
    [Google Scholar]
  17. Fournier J. G., Tardieu M., Lebon P., Robain O., Ponsot G., Rozenblatt S., Bouteille M. 1985; Detection of measles virus RNA in lymphocytes from peripheral-blood and brain perivascular infiltrates of patients with subacute sclerosing panencephalitis. New England Journal of Medicine 313:910–915
    [Google Scholar]
  18. Gillet J. P., Derer P., Tsiang H. 1986; Axonal transport of rabies virus in the central nervous system of the rat. Journal of Neuropathology and Experimental Neurology 17:44–53
    [Google Scholar]
  19. Goldstein G. W., Betz A. L. 1986; The blood brain barrier. Scientific American 9:70–79
    [Google Scholar]
  20. Haase A. T., Brahic M., Stowring L., Blum H. 1984; Detection of viral nucleic acids by in situ hybridization. Methods in Virology 7:189–226
    [Google Scholar]
  21. Hashimoto I., Hagiwara A., Komatsu T. 1984; Ultrastructural studies on the pathogenesis of poliomyelitis in monkeys infected with poliovirus. Acta neuropathologica 64:53–60
    [Google Scholar]
  22. Janzer R. C., Raff M. C. 1987; Astrocytes induce blood brain barrier properties in endothelial cells. Nature London: 325253–257
    [Google Scholar]
  23. Jubelt B., Gallez-hawaking G., Narayan O., Johnson R. T. 1980a; Pathogenesis of human poliovirus infection in mice. I. Clinical and pathological studies. Journal of Neuropathology and Experimental Neurology 39:138–148
    [Google Scholar]
  24. Jubelt B., Narayan O., Johnson R. T. 1980b; Pathogenesis of human poliovirus infection in mice. II. Age-dependency of paralysis. Journal of Neuropathology and Experimental Neurology 38:149–159
    [Google Scholar]
  25. Jubelt B., Goldfarb S. J., Paradise M. J., Close M. G. 1986; Fast axonal transport of human poliovirus (HPV). (Abstract). Neurology 36:1204
    [Google Scholar]
  26. Kanamitsu K., Kasamaki A., Ogawa M., Kasahara S., Imanura M. 1967; Immunofluorescent study on the pathogenesis of oral infection of poliovirus in monkeys. Japanese Journal of Medical Science and Biology 20:175–194
    [Google Scholar]
  27. Kean K M., Wychowski C., Kopecka H., Girard M. 1986; Highly infectious plasmids carrying poliovirus cDNA are capable of replication in transfected simian cells. Journal of Virology 59:490–493
    [Google Scholar]
  28. Kovacks E., Baratawidjaja K. K., Labzoffsky A. 1963; Visualization of poliovirus type III in paraffin sections of monkeys spinal cord by indirect immunofluorescence. Nature London: 200497–498
    [Google Scholar]
  29. Kristensson K., Lycke E. 1971; Spread of herpes simplex virus in peripheral nerves. Ada neuropathologica 17:44–53
    [Google Scholar]
  30. La monica N., Racaniello V. R. 1989; Differences in replication of attenuated and neurovirulent polioviruses in human neuroblastoma cell line SH-SY5Y. Journal of Virology 63:2357–2360
    [Google Scholar]
  31. Mendelsohn C. L., Wimmer E., Racaniello V. R. 1989; Cellular receptor for poliovirus: molecular cloning,nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell 56:855–865
    [Google Scholar]
  32. Moench T. R., Gendelman H. E., Clements J. E., Narayan O., Griffin D. E. 1985; Efficiency of in situ hybridization as a function of probe size and fixation technique. Journal of Virological Methods 11:119–130
    [Google Scholar]
  33. Murphy F. A., Bauer S. P., Harisson K., Winn W. C. 1973; Comparative pathogenesis of rabies and rabies-like viruses. Viral infection transit from inoculation site to the central nervous system. Laboratory Investigation 28:361–376
    [Google Scholar]
  34. Raff M. C. 1989; Glial cell diversification in the rat optic nerve. Science 243:1450–1455
    [Google Scholar]
  35. Sabin A. B. 1956; Pathogenesis of poliomyelitis: reappraisal in the light of new data. Science 123:1151–1157
    [Google Scholar]
  36. Shepley M. P., Sherry B., Weiner H. L. 1988; Monoclonal antibody identification of a 100-kDa membrane protein in HeLa cells and human spinal cord involved in poliovirus attachment. Proceedings of the National Academy of SciencesU.S.A 857743–7747
    [Google Scholar]
  37. Simon J., Magrath D., Boulger L. 1970; The role of the defensive mechanism in experimental poliomyelitis. Progress in Immunobiological Standardization 4:643–649
    [Google Scholar]
  38. Stroop W. G., Baringer J. R., Brahic M. 1981; Detection of Theiler’s virus RNA in mouse central nervous system by in situ hybridization. Laboratory Investigation 45:504–509
    [Google Scholar]
  39. Szabo P., Edler R., Steffensen D. M., Uhlenbeck O. C. 1977; Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster. Journal of Molecular Biology 115:539–563
    [Google Scholar]
  40. Thomas P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of SciencesU.S.A 775201–5205
    [Google Scholar]
  41. world health organization 1981; WHO expert committee on biological standardization. Technical Report Series 658:157–163
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-70-11-2907
Loading
/content/journal/jgv/10.1099/0022-1317-70-11-2907
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error