1887

Abstract

Summary

Spleen cells from BALB/c (H-2) mice vaccinated with vgB11, a recombinant vaccinia virus which expresses glycoprotein B (gB) of herpes simplex virus type 1 (HSV-1), lysed EMT6 (H-2) target cells infected with vgB11 or with HSV-1 but did not lyse uninfected EMT6 cells or infected L-929 (H-2) target cells. Unlabelled target cell competition of lysis showed that only syngeneic cells infected with vgB11 or HSV-1 inhibited lysis of radiolabelled HSV-1-infected targets. These results demonstrate that vgB11 induces H-2-restricted anti-HSV-1 cytotoxic T lymphocytes and that gB is the target antigen.

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1988-07-01
2024-04-26
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References

  1. BENNINK J. R., YEWDELL J. W., SMITH G. L., MOLLER C., MOSS B. 1984; Recombinant vaccinia virus primes and stimulates influenza haemagglutinin-specific cytotoxic T cells. Nature, London 311:578–579
    [Google Scholar]
  2. BLACKLAWS B. A., NASH A. A., DARBY G. 1987; Specificity of the immune response of mice to herpes simplex virus glycoproteins B and D constitutively expressed on L cell lines. Journal of General Virology 68:1103–1114
    [Google Scholar]
  3. CANTIN E. M., EBERLE R., BALDICK J. L., MOSS B., WILLEY D. E., NOTKINS A. L., OPENSHAW H. 1987; Expression of herpes simplex virus 1 glycoprotein B by a recombinant vaccinia virus and protection of mice against lethal herpes simplex virus 1 infection. Proceedings of the National Academy of Sciences, U.S.A 84:5908–5912
    [Google Scholar]
  4. CARTER V. C, SCHAFFER P. A., TEVETHIA S. S. 1981; The involvement of herpes simplex virus type 1 glycoproteins in cell-mediated immunity. Journal of Immunology 126:1655–1600
    [Google Scholar]
  5. CHAKRABARTI S., BRECHLING K., MOSS B. 1985; Vaccinia virus expression vector: coexpression of /J-galactosidase provides visual screening of recombinant virus plaques. Molecular and Cellular Biology 5:3403–3409
    [Google Scholar]
  6. COUPAR B. E., ANDREW M. E., BOTH G. W., BOYLE D. B. 1986; Temporal regulation of influenza hemagglutinin expression in vaccinia virus recombinants and effects on the immune response. European Journal of Immunology 16:1479–1487
    [Google Scholar]
  7. CREMER K. J., MACKETT M., WOHLENBERG C, NOTKINS A. L., MOSS B. 1985; Vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D prevents latent herpes in mice. Science 228:737–740
    [Google Scholar]
  8. GLORIOSO J., KEES U., KUMEL G., KIRSCHNER H., KRAMMER P. H. 1985; Identification of herpes simplex virus type 1 (HSV-1) glycoprotein gC as the immunodominant antigen for HSV-1-specific memory cytotoxic T lymphocytes. Journal of Immunology 135:575–582
    [Google Scholar]
  9. LAWMAN M. J. P., COURTNEY R. J., EBERLE R., SCHAFFER P. A., O’HARA M. K., ROUSE B. T. 1985; Cell-mediated immunity to herpes simplex virus: specificity of cytotoxic T cells. Infection and Immunity 30:451–461
    [Google Scholar]
  10. MARTIN S., ROUSE B. T. 1987; The mechanisms of antiviral immunity induced by a vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D: clearance of local infection. Journal of Immunology 138:3431–3437
    [Google Scholar]
  11. MARTIN S., MOSS B., BERMAN P. W., LASKEY L. A., ROUSE B. 1987; Mechanisms of antiviral immunity induced by a vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D: cytotoxic T cells. Journal of Virology 61:726–734
    [Google Scholar]
  12. NASH A. A., LEUNG K-L., WILDY P. 1985 The T-cell-mediated immune response of mice to herpes simplex virus. The Herpesviruses 487–102 Edited by Roizman B., Lopez C. New York & London: Plenum Press;
    [Google Scholar]
  13. ROSENTHAL K. L., SMILEY J. R., SOUTH S., JOHNSON D. C. 1987; Cells expressing herpes simplex virus glycoprotein gC but not gB, gD, or gE are recognized by murine virus-specific cytotoxic T lymphocytes. Journal of Virology 61:2438–2447
    [Google Scholar]
  14. SMITH G. L., MURPHY B. R., MOSS B. 1983; Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza virus hemagglutinin gene and induces resistance to influenza infection in hamsters. Proceedings of the National Academy of Sciences, U.S.A 80:7155–7159
    [Google Scholar]
  15. WIKTOR T. J., MACFARLAN R. I., REAGAN K. J., DIETZSCHOLD B., CURTIS P. J., WUNNER W. H., KIENY M-P., LATHE R., LECOCQ J-P., MACKETT M., MOSS B., KOPROWSKI H. 1984; Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene. Proceedings of the National Academy of Sciences, U.S.A 81:7194–7198
    [Google Scholar]
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