1887

Abstract

Summary

Thirteen monoclonal antibodies (MAbs) were prepared against human parainfluenza virus type 2 (PIV2). These MAbs reacted with the haemagglutinin-neuraminidase glycoprotein with an of 84K. The MAbs defined one antigenic site which could be divided into five epitopes. A correlation between haemagglutination inhibition (HI) and neutralization activity could be seen although one MAb, which recognized a distinct epitope, showed neutralization and no HI activity to PIV2. The reactivity of the MAbs was tested against Sendai virus, parainfluenza virus type 3, simian virus 5 (SV5), mumps virus, Newcastle disease virus, measles virus and canine distemper virus. Only one MAb showed any cross-reaction with a low HI titre to SV5.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-4-931
1988-04-01
2024-06-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/4/JV0690040931.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-4-931&mimeType=html&fmt=ahah

References

  1. AMIT A. G., MARIUZZA R. A., PHILLIPS S. E. V., POLJAK R. J. 1986; Three-dimensional structure of an antigen-antibody complex at 2-8 À resolution. Science 233:747–753
    [Google Scholar]
  2. ATASSI M. Z. 1978; Precise determination of the entire antigenic structure of lysozyme: molecular features of protein antigenic structures and potential of “surface-simulation” synthesis - a powerful new concept for protein binding sites. Immunochemistry 15:909–936
    [Google Scholar]
  3. CHOPPIN P. W., SCHEID A. 1980; The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Review of Infectious Diseases 2:40–61
    [Google Scholar]
  4. COELINGH K. L., WINTER C. C, MURPHY B. R., RICE J. M., KIMBALL P. C, OLMSTED R. A., COLLINS P. L. 1986; Conserved epitopes on the hemagglutinin-neuraminidase proteins of human and bovine parainfluenza type 3 viruses: nucleotide sequence analysis of variants selected with monoclonal antibodies. Journal of Virology 60:90–96
    [Google Scholar]
  5. COLMAN P. M., LAVER W. G., VARGHESE J. N., BAKER A. T., TULOCH P. A., AIR G. M., WEBSTER R. G. 1987; Three-dimensional structure of a complex of antibody with influenza virus neuraminidase. Nature, London 326:358–363
    [Google Scholar]
  6. COWLEY J. A., BARRY R. D. 1983; Characterization of human parainfluenza viruses. I. The structural proteins of parainfluenza virus 2 and their synthesis in infected cells. Journal of General Virology 64:2117–2125
    [Google Scholar]
  7. DIMMOCK N. J. 1984; Mechanisms of neutralization of animal viruses. Journal of General Virology 65:1015–1022
    [Google Scholar]
  8. GLEZEN W. P., LODA F. A., DENNY F. A. 1982 Parainfluenza viruses. Viral Infections of Humans, 2nd edn.441–445 Edited by Evans A. S. New York: Plenum Press;
    [Google Scholar]
  9. GOSWAMI K. K. A., RUSSELL W. C. 1982; A comparison of paramyxoviruses by immunoprecipitation. Journal of General Virology 60:177–183
    [Google Scholar]
  10. GOSWAMI K. K. A., RUSSELL W. C. 1983; Monoclonal antibodies against human paramyxovirus type 3 and against SV5 virus: preparation and preliminary characterization. Journal of General Virology 64:1663–1672
    [Google Scholar]
  11. HOGLE J. M., CHOW M., FILMAN D. J. 1985; Three-dimensional structure of poliovirus at 2-9 Â resolution. Science 229:1358–1365
    [Google Scholar]
  12. HUBER R., BENNET W. S. 1987; Antibody-antigen flexibility. Nature, London 326:334–335
    [Google Scholar]
  13. ITO Y., TSURUDOME M., HISHIYAMA M., YAMADA A. 1987a; Immunological interrelationships among human and non-human paramyxoviruses revealed by immunoprecipitation. Journal of General Virology 68:1289–1297
    [Google Scholar]
  14. ITO Y., TSURUDOME M., HISHIYAMA M. 1987b; The polypeptides of human parainfluenza type 2 virus and their synthesis in infected cells. Archives of Virology 95:211–224
    [Google Scholar]
  15. KINGSBURY D. W., BRATT M. A., CHOPPIN P. W., HANSON R. P., HOSAKA Y., TER MEULEN V., NORRBY E., PLOWRIGHT W., ROTT R., WUNNER W. H. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  16. KÖHLER G., MILSTEIN C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:A95–A91
    [Google Scholar]
  17. LOVE A., RYDBECK R., LJUNGDAHL Â., KRISTENSSON K., NORRBY E. 1986; Selection of mutants of mumps virus with altered structure and pathogenicity by passage in vivo. Microbial Pathogenesis 1:149–158
    [Google Scholar]
  18. NORRBY E. 1962; Hemagglutination by measles virus. IV. A simple procedure for production of high potency antigen for hemagglutination inhibition (HI) tests. Proceedings of the Society for Experimental Biology and Medicine 111:814–818
    [Google Scholar]
  19. ÖRVELL C. 1976; Identification of paramyxovirus specific haemolysis-inhibiting antibodies separate from haemagglutination-inhibiting and neuraminidase-inhibiting antibodies. 2. NDV and mumps virus haemolysis-inhibiting antibodies. Acta pathologica et microbiologica scandinavica section B 84:451–457
    [Google Scholar]
  20. ÖRVELL C. 1984; The reactions of monoclonal antibodies with structural proteins of mumps virus. Journal of Immunology 132:2622–2629
    [Google Scholar]
  21. ÖRVELL C., GRANDIEN M. 1982; The effects of monoclonal antibodies on biological activities of structural proteins of Sendai virus. Journal of Immunology 129:2779–2787
    [Google Scholar]
  22. ÖRVELL C., NORRBY E. 1980; Immunological relationships between homologous polypeptides of measles and canine distemper virus. Journal of General Virology 50:231–245
    [Google Scholar]
  23. ÖRVELL C, RYDBECK R., LOVE A. 1986; Immunological relationships between mumps virus and parainfluenza viruses studied with monoclonal antibodies. Journal of General Virology 67:1929–1939
    [Google Scholar]
  24. RYDBECK R., LöVE A., ÖRVELL C, NORRBY E. 1986a; Antigenic variation of envelope and internal proteins of mumps virus strains detected with monoclonal antibodies. Journal of General Virology 67:281–287
    [Google Scholar]
  25. RYDBECK R., öRVELL C, LOVE A., NORRBY E. 1986b; Characterizaton of four parainfluenza virus type 3 proteins by use of monoclonal antibodies. Journal of General Virology 67:1531–1542
    [Google Scholar]
  26. RYDBECK R., LöVE A., öRVELL C., NORRBY E. 1987; Antigenic analysis of human and bovine parainfluenza virus type 3 strains with monoclonal antibodies. Journal of General Virology 68:2153–2160
    [Google Scholar]
  27. SHESHBERADARAN H., CHEN S. N., NORRBY E. 1983; Monoclonal antibodies against five structural components of measles virus. 1. Characterization of antigenic determinants on nine strains of measles virus. Virology 128:341–353
    [Google Scholar]
  28. VAN WYKE COELINGH K. L., WINTER C., MURPHY B. 1985; Antigenic variation in the hemagglutinin-neuraminidase protein of human parainfluenza type 3 virus. Virology 143:569–582
    [Google Scholar]
  29. WILSON B. M., NAKANE P. K. 1978 Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. Immunofluorescence and Related Staining Techniques215 Edited by Knapp W., Holubat K., Wick G. Amsterdam: Elsevier/North-Holland;
    [Google Scholar]
  30. YEWDELL J. W., GERHARD W. 1981; Antigenic characterization of viruses by monoclonal antibodies. Annual Review of Microbiology 35:185–206
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-4-931
Loading
/content/journal/jgv/10.1099/0022-1317-69-4-931
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error