1887

Abstract

Summary

High molecular weight human cytomegalovirus (CMV) DNA was isolated from agarose-embedded infected human diploid cells by employing field inversion gel electrophoresis. A high yield of CMV DNA molecules was obtained within 1 week of infecting the cell culture. Labelling of the viral DNA with biotin by nick translation enabled the detection of CMV-infected cells in sections of paraffin-embedded human adrenal gland by hybridization.

Keyword(s): CMV DNA , FIGE and in situ hybridization
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/content/journal/jgv/10.1099/0022-1317-69-3-699
1988-03-01
2021-10-26
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References

  1. BOOM R., GEELEN J. L. M. C, SOL C. J., RAAP A. J., MINNAAR R. P., KLAVER B. P., VAN DER NOORDAA J. 1986; Establishment of a rat cell line inducible for the expression of human cytomegalovirus immediate-early gene products by protein synthesis inhibition. Journal of Virology 58:851–859
    [Google Scholar]
  2. CARLE G. F., OLSON M. V. 1984; Separation of chromosomal DNA molecules from yeast by orthogonal-field-alteration gel electrophoresis. Nucleic Acids Research 12:5647–5664
    [Google Scholar]
  3. CARLE G. F., FRANK M., OLSON M. V. 1986; Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science 232:65–68
    [Google Scholar]
  4. FREIFELDER D. 1970; Molecular weights of coliphages and coliphage DNA. IV. Molecular weight of the DNAs from bacteriophage T4, T5 and T7 and the general problem of determination of M. Journal of Molecular Biology 54:567–577
    [Google Scholar]
  5. GEELEN J. L. M. C, WALIG C, WERTHEIM P., VAN DER NOORDAA J. 1978; Human cytomegalovirus DNA. I. Molecular weight and infectivity. Journal of Virology 26:813–816
    [Google Scholar]
  6. HUANG E. S., CHEN S. T., PAGANO J. S. 1973; Human cytomegalovirus. I. Purification and characterization of viral DNA. Journal of Virology 12:1473–1481
    [Google Scholar]
  7. LONING T., MILDE K., FOSS H. D. 1986; In situ hybridization for the detection of cytomegalovirus (CMV) infection. Application of biotinylated CMV-DNA probes on paraffin embedded specimen. Virchows Archiv 409:777–790
    [Google Scholar]
  8. LÜLECI G., SAKîZLî, M. & GÜNALP A. 1980; Selective chromosomal damage caused by human cytomegalovirus. Acta virologica 24:341–345
    [Google Scholar]
  9. MANIATIS T., FRITSCH E. F., SAMBROOK J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. RAAP A. K., GEELEN J. L. M. C, VAN DER MEER J. W. M., DE RYKE F. M., VAN DE BOOGAART P., VAN DER PLOEG M. 1987; Non-radioactive in situ hybridization for the detection of cytomegalovirus infections. Histochemistry in press
    [Google Scholar]
  11. ROIZMAN B. 1979; The structure and isomerization of herpes simplex virus genomes. Cell 16:481–494
    [Google Scholar]
  12. SPAETE R. R., MOCARSKI E. S. 1985; The a sequence of the cytomegalovirus genome functions as a cleavage/packaging signal for herpes simplex virus defective genomes. Journal of Virology 54:817–824
    [Google Scholar]
  13. STINSKI M. F., MOCARSKI E. S., THOMSEN D. R. 1979; DNA of defective cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage. Journal of Virology 31:231–239
    [Google Scholar]
  14. VAN DEN BERG F. M., VAN AMSTEL P. J., WALBOOMERS J. M. M. 1985; Construction of rat cell lines that contain potential morphologically transforming regions of the HSV-2 genome. Intervirology 24:199–210
    [Google Scholar]
  15. VAN DER PLOEG L. T. H., SCHWARTZ D. C, CANTOR C. R., BORST P. 1984; Antigenic variation in Trypanosoma brucei analyzed by electrophoretic separation of chromosome-sized DNA molecules. Cell 37:77–84
    [Google Scholar]
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