1887

Abstract

Summary

We analysed serotype-specific antigens of human rotavirus (HRV) by the use of neutralizing monoclonal antibodies (N-MAbs). The reactivity patterns of 12 serotype-specific N-MAbs against 15 HRV strains in a neutralization test revealed great intraserotypic antigenic variation especially those belonging to serotypes 2, 3 and 4. On the basis of the protein specificity of the antibodies examined, it was suggested that whereas serotype 2-specific neutralization epitopes were present on both VP3 and VP7 outer capsid proteins, serotype 1-, 3- and 4-specific neutralization epitopes were located on VP7. Serotype-specific neutralization epitopes on VP7 of the serotype 1 HRV KU strain were analysed further using mutants of the KU strain resistant to different serotype 1-specific N-MAbs. The result suggested the presence of at least five operationally overlapping neutralization epitopes on VP7 of the KU strain, which collectively constituted a single large neutralization domain.

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1988-02-01
2022-01-24
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References

  1. COULSON B. S., TURSI J. M., McADAM W. J., BISHOP R. F. 1986; Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. Virology 154:302–312
    [Google Scholar]
  2. DYALL-SMITH M. L., LAZDINS I., TREGEAR G. W., HOLMES I. H. 1986; Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proceedings of the National Academy of Sciences, U.S.A 83:3465–3468
    [Google Scholar]
  3. ESTES M. K., PALMER E. L., OBUESKI J. F. 1983; Rotaviruses: a review. Current Topics in Microbiology and Immunology 105:123–184
    [Google Scholar]
  4. GREENBERG H. B., FLORES J., KALICA A. R., WYATT R. G., JONES R. 1983; Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. Journal of General Virology 64:313–320
    [Google Scholar]
  5. HOSHINO Y., SERENO M. M., MIDTHUN K., FLORES J., KAPIKIAN A. Z., CHANOCK R. M. 1985; Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proceedings of the National Academy of Sciences, U.S.A 82:8701–8704
    [Google Scholar]
  6. KALICA A. R., GREENBERG H. B., WYATT R. G., FLORES J., SERENO M. M., KAPIKIAN A. Z., CHANOCK R. M. 1981; Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112:385–390
    [Google Scholar]
  7. LAZDINS I., SONZA S., DYALL-SMITH M. L., COULSON B. S., HOLMES I. H. 1985; Demonstration of an immunodominant neutralization site by analysis of antigenic variants of SA 11 rotavirus. Journal of Virology 56:317–319
    [Google Scholar]
  8. LüBECK M. D., GERHARD W. 1981; Topological mapping of antigenic sites on the influenza A/PR/8/34 virus hemagglutinin using monoclonal antibodies. Virology 113:64–72
    [Google Scholar]
  9. McCRAE M. A., McCORQUODALE J. G. 1982; The molecular biology of rotaviruses. II. Identification of the protein-coding assignments of calf rotavirus genome RNA species. Virology 117:435–443
    [Google Scholar]
  10. MINOR P. D., SCHILD G. C, BOOTMAN J., EVANS D. M. A., FERGUSON M., REEVE P., SPITZ M., STANWAY G., CANN A. J., HAUPTMANN R., CLARKE L. D., MOUNTFORD R. C, ALMOND J. W. 1983; Location and primary structure of a major antigenic site for poliovirus neutralization. Nature, London 301:674–679
    [Google Scholar]
  11. OFFIT P. A., BLAVAT G. 1986; Identification of two rotavirus genes determining neutralization specificities. Journal of Virology 57:376–378
    [Google Scholar]
  12. SABARA M., GILCHRIST J. E., HUDSON G. R., BABIUK L. A. 1985; Preliminary characterization of an epitope involved in neutralization and cell attachment that is located on the major bovine rotavirus glycoprotein. Journal of Virology 53:58–66
    [Google Scholar]
  13. SATO K., INABA Y., SHINOZAKI T., FUJII R., MATUMOTO M. 1981; Isolation of human rotavirus in cell cultures. Archives of Virology 69:155–160
    [Google Scholar]
  14. SHAW R. D., VO P. T., OFFIT P. A., COULSON B. S., GREENBERG H. B. 1986; Antigenic mapping of the surface proteins of rhesus rotavirus. Virology 155:434–451
    [Google Scholar]
  15. SMITH M. L., LAZDINS I., HOLMES I. H. 1980; Coding assignments of double-stranded RNA segments of SA11 rotavirus established by in vitro translation. Journal of Virology 33:976–982
    [Google Scholar]
  16. SONZA S., BRESCHKIN A. M., HOLMES I. H. 1984; The major surface glycoprotein of simian rotavirus (SA11) contains distinct epitopes. Virology 134:318–327
    [Google Scholar]
  17. TANIGUCHI K., URASAWA T., URASAWA S., YASUHARA T. 1984; Production of subgroup-specific monoclonal antibodies against human rotaviruses and their application to enzyme-linked immunosorbent assay for subgroup determination. Journal of Medical Virology 14:115–125
    [Google Scholar]
  18. TANIGUCHI K., URASAWA S., URASAWA T. 1985; Preparation and characterization of neutralizing monoclonal antibodies with different reactivity patterns to human rotaviruses. Journal of General Virology 66:1045–1053
    [Google Scholar]
  19. TANIGUCHI K., MORITA Y., URASAWA T., URASAWA S. 1987a; Cross-reactive neutralization epitopes on VP3 of human rotavirus: analysis with monoclonal antibodies and antigenic variants. Journal of Virology 61:1726–1730
    [Google Scholar]
  20. TANIGUCHI K., URASAWA T., MORITA Y., URASAWA S. 1987b; Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1, 2, 3 and 4-specific monoclonal antibodies directed to VP7. Journal of Infectious Diseases 155:1159–1166
    [Google Scholar]
  21. URASAWA S., URASAWA T., TANIGUCHI K. 1982; Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains. Infection and Immunity 38:781–784
    [Google Scholar]
  22. URASAWA S., URASAWA T., TANIGUCHI K., CHIBA S. 1984; Serotype determination of human rotavirus isolates and antibody prevalence in pediatric population in Hokkaido, Japan. Archives of Virology 81:1–12
    [Google Scholar]
  23. URASAWA S., URASAWA T., TANIGUCHI K. 1986; Genetic reassortment between two human rotaviruses having different serotype and subgroup specificities. Journal of General Virology 67:1551–1559
    [Google Scholar]
  24. URASAWA T., URASAWA S., TANIGUCHI K. 1981; Sequential passages of human rotavirus in MA-104 cells. Microbiology and Immunology 25:1025–1035
    [Google Scholar]
  25. URASAWA T., URASAWA S., CHIBA Y., TANIGUCHI K., KOBAYASHI N., MUTANDA L. N., TUKEI P. M. 1987; Antigenic characterization of rotaviruses isolated in Kenya from 1982 to 1983. Journal of Clinical Microbiology 25:1891–1896
    [Google Scholar]
  26. WIKTOR T. J., KOPROWSKI H. 1980; Antigenic variants of rabies virus. Journal of Experimental Medicine 152:99–112
    [Google Scholar]
  27. WYATT R. G., JAMES H. D. JR, PITTMAN A. L., HOSHINO Y., GREENBERG H. B., KALICA A. R., FLORES J., KAPIKIAN A. Z. 1983; Direct isolation in cell culture of human rotaviruses and characterization into four serotypes. Journal of Clinical Microbiology 18:310–317
    [Google Scholar]
  28. YEWDELL J. W., GERHARD W. 1981; Antigenic characterization of viruses by monoclonal antibodies. Annual Review of Microbiology 35:185–206
    [Google Scholar]
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