Synchronous multiplication of rice gall dwarf virus (RGDV) and an assay of its infectivity in cell monolayers of its vector, the green rice leafhopper , are described. The number of foci of infected cells was linearly related to the concentration of virus. The method was about 10 times more sensitive than enzymelinked immunosorbent assay. All the vector cells in monolayers were infected when inocula were dilutions of 10 of sap from infected plants, 10 of extracts of viruliferous insects, 10 of infected monolayer cells or purified virus with = 10. RGDV was first detected in monolayer cells 10 h after inoculation, and multiplied 10-fold in the subsequent 40 h. Vector cell monolayers are thus an excellent experimental system for the study of RGDV.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error